|
|
 Previous Article | Table of Contents | Next Article 
In Vitro Negative Selection of Viral Superantigen-Reactive Thymocytes by Thymic Dendritic Cells
Isabel Ferrero,
Fabienne Anjuère,
H. Robson MacDonald, and
Carlos Ardavín
From the Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain, and the Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Epalinges, Switzerland.
Intrathymic expression of endogenous mouse mammary tumor virus (MMTV)-encoded superantigens (SAg) induces the clonal deletion of T cells bearing SAg-reactive T-cell receptor (TCR) V elements. However, the identity of the thymic antigen-presenting cells (APC) involved in the induction of SAg tolerance remains to be defined. We have analyzed the potential of dendritic cells (DC) to mediate the clonal deletion of Mtv-7-reactive TCR  P14 transgenic thymocytes in an in vitro assay. Our results show that both thymic and splenic DC induced the deletion of TCR transgenic double positive (DP) thymocytes. DC appear to be more efficient than splenic B cells as negatively selecting APC in this experimental system. Interestingly, thymic and splenic DC display a differential ability to induce CD4+SP thymocyte proliferation. These observations suggest that thymic DC may have an important role in the induction of SAg tolerance in vivo.
Blood, Vol. 90 No. 5 (September 1), 1997:
pp. 1943-1951
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P. Martin, S. R. Ruiz, G. M. del Hoyo, F. Anjuere, H. H. Vargas, M. Lopez-Bravo, and C. Ardavin
Dramatic increase in lymph node dendritic cell number during infection by the mouse mammary tumor virus occurs by a CD62L-dependent blood-borne DC recruitment
Blood,
February 15, 2002;
99(4):
1282 - 1288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Kleindienst, I. Chretien, T. Winkler, and T. Brocker
Functional comparison of thymic B cells and dendritic cells in vivo
Blood,
April 15, 2000;
95(8):
2610 - 2616.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Anjuere, P. Martin, I. Ferrero, M. L. Fraga, G. M. del Hoyo, N. Wright, and C. Ardavin
Definition of Dendritic Cell Subpopulations Present in the Spleen, Peyer's Patches, Lymph Nodes, and Skin of the Mouse
Blood,
January 15, 1999;
93(2):
590 - 598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Ferrero, F. Anjuere, I. Azcoitia, T. Renno, H. R. MacDonald, and C. Ardavin
Viral Superantigen-Induced Negative Selection of TCR Transgenic CD4+ CD8+ Thymocytes Depends on Activation, but not Proliferation
Blood,
June 1, 1998;
91(11):
4248 - 4254.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
