|
|
 Previous Article | Table of Contents | Next Article 
Growth of Human T-Cell Lineage Acute Leukemia in Severe Combined Immunodeficiency (SCID) Mice and Non-obese Diabetic SCID Mice
Jeremy P.C. Steele,
Robyn D. Clutterbuck,
Ray L. Powles,
Paul L.R. Mitchell,
Clive Horton,
Ricardo Morilla,
Daniel Catovsky, and
John L. Millar
From the Leukaemia Unit and Academic Department of Haematology and Cytogenetics, The Institute of Cancer Research and Royal Marsden National Health Service Trust, Sutton, Surrey, UK.
Primary leukemic cells from patients with acute lymphoblastic leukemia (ALL) can be injected intravenously into mice with severe combined immunodeficiency (SCID) to create a model of human leukemia. Leukemic cells disseminate to murine tissues in a clinicopathologic pattern similar to that seen in humans. Thus far, reports of engraftment of lymphoid leukemia in SCID mice have mainly been from patients with B-cell lineage ALL, for which engraftment occurs more frequently with cells from high-risk patients. There are few data on the engraftment of T-cell lineage ALL in SCID mice. Leukemic cells from 19 patients (16 adult and three pediatric) with T-cell lineage ALL were injected into SCID mice, with overt engraftment of 12 cases (63%). Engraftment of leukemia in SCID mice was associated with earlier death due to leukemia of the patient donors (P < .01, log-rank test). The recently developed non-obese diabetic (NOD)/SCID mouse may expand the uses of the SCID model. Cells from the seven patients with T-cell lineage ALL that failed to cause leukemia in SCID mice were injected into NOD/SCID mice. Overt leukemia engraftment was observed in all seven cases. Thus, growth of human T-cell lineage ALL cells in SCID mice was associated with a high-risk patient group. However, this association was not observed when NOD/SCID mice were used, suggesting that this model would no longer predict patients likely to die early of leukemia, but may provide a more realistic system for studying the biology and treatment of the disease.
Blood, Vol. 90 No. 5 (September 1), 1997:
pp. 2015-2019
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. V. Cox, H. M. Martin, P. R. Kearns, P. Virgo, R. S. Evely, and A. Blair
Characterization of a progenitor cell population in childhood T-cell acute lymphoblastic leukemia
Blood,
January 15, 2007;
109(2):
674 - 682.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. L. M. Liem, R. A. Papa, C. G. Milross, M. A. Schmid, M. Tajbakhsh, S. Choi, C. D. Ramirez, A. M. Rice, M. Haber, M. D. Norris, et al.
Characterization of childhood acute lymphoblastic leukemia xenograft models for the preclinical evaluation of new therapies
Blood,
May 15, 2004;
103(10):
3905 - 3914.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. B. Lock, N. Liem, M. L. Farnsworth, C. G. Milross, C. Xue, M. Tajbakhsh, M. Haber, M. D. Norris, G. M. Marshall, and A. M. Rice
The nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse model of childhood acute lymphoblastic leukemia reveals intrinsic differences in biologic characteristics at diagnosis and relapse
Blood,
May 13, 2002;
99(11):
4100 - 4108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bazin, E. Aubin, L. Boyer, I. St-Amour, C. Roberge, and R. Lemieux
Functional in vivo characterization of human monoclonal anti-D in NOD-scid mice
Blood,
February 15, 2002;
99(4):
1267 - 1272.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. P. Dialynas, M.-J. Lee, D. P. Gold, L.-e. Shao, A. L. Yu, M. J. Borowitz, and J. Yu
Preconditioning with fetal cord blood facilitates engraftment of primary childhood T-cell acute lymphoblastic leukemia in immunodeficient mice
Blood,
May 15, 2001;
97(10):
3218 - 3225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Perez, H. M. Rinder, C. Wang, J. B. Tracey, N. Maun, and D. S. Krause
Xenotransplantation of immunodeficient mice with mobilized human blood CD34+ cells provides an in vivo model for human megakaryocytopoiesis and platelet production
Blood,
March 15, 2001;
97(6):
1635 - 1643.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Fusetti, G. Pruneri, A. Gobbi, C. Rabascio, N. Carboni, F. Peccatori, G. Martinelli, and F. Bertolini
Human Myeloid and Lymphoid Malignancies in the Non-Obese Diabetic/Severe Combined Immunodeficiency Mouse Model: Frequency of Apoptotic Cells in Solid Tumors and Efficiency and Speed of Engraftment Correlate with Vascular Endothelial Growth Factor Production
Cancer Res.,
May 1, 2000;
60(9):
2527 - 2534.
[Abstract]
[Full Text]
|
|
|
|
|
