|
|
 Previous Article | Table of Contents | Next Article 
CD34 Expression Patterns During Early Mouse Development Are Related to Modes of Blood Vessel Formation and Reveal Additional Sites of Hematopoiesis
Heather B. Wood,
Gillian May,
Lyn Healy,
Tariq Enver, and
Gillian M. Morriss-Kay
From the Department of Human Anatomy, University of Oxford, Oxford; and The Leukaemia Research Fund Centre at The Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
CD34 is a cell surface glycoprotein that is selectively expressed within the human hematopoietic system on stem and progenitor cells, and in early blood vessels. To elucidate its functions during early blood vessel formation and hematopoiesis, we analyzed the expression patterns, in day 8 to day 10 mouse embryos, of CD34 RNA by in situ hybridization and protein by immunohistochemistry using the monclonal antibody RAM 34. Levels of expression in embryonic blood vessels were correlated with the mode of vessel formation, being high in pre-endothelial cells and in vessels forming by vasculogenesis (particularly the dorsal aortae) or angiogenesis, but low in vessels forming by coalescence (the cardinal veins). CD34+ erythroid cells, presumably of yolk sac origin, were present in the liver of day 10 embryos; at the same stage, putative definitive hematopoietic cells, strongly CD34+, were present in the para-aortic mesenchyme. Possible sites of hemangioblastic differentiation were detected in the form of CD34+ endothelium-attached hematopoietic cells in the dorsal aorta and in two previously unreported locations, the proximal umbilical and vitelline arteries. These observations suggest functions for CD34 in relation to specific modes of blood vessel formation, and a hemangioblastic role in both embryonic and extraembryonic sites.
Blood, Vol. 90 No. 6 (September 15), 1997:
pp. 2300-2311
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. E. Testa, A. Chrastina, P. Oh, Y. Li, H. Witkiewicz, M. Czarny, T. Buss, and J. E. Schnitzer
Immunotargeting and cloning of two CD34 variants exhibiting restricted expression in adult rat endothelia in vivo
Am J Physiol Lung Cell Mol Physiol,
August 1, 2009;
297(2):
L251 - L262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Acevedo, I. Londono, M. Oubaha, L. Ghitescu, and M. Bendayan
Glomerular CD34 Expression in Short- and Long-term Diabetes
J. Histochem. Cytochem.,
June 1, 2008;
56(6):
605 - 614.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ema, T. Yokomizo, A. Wakamatsu, T. Terunuma, M. Yamamoto, and S. Takahashi
Primitive erythropoiesis from mesodermal precursors expressing VE-cadherin, PECAM-1, Tie2, endoglin, and CD34 in the mouse embryo
Blood,
December 15, 2006;
108(13):
4018 - 4024.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Gribi, L. Hook, J. Ure, and A. Medvinsky
The differentiation program of embryonic definitive hematopoietic stem cells is largely {alpha}4 integrin independent
Blood,
July 15, 2006;
108(2):
501 - 509.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. V. Cardoso and J. Lu
Regulation of early lung morphogenesis: questions, facts and controversies
Development,
May 1, 2006;
133(9):
1611 - 1624.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hirai, I. M Samokhvalov, T. Fujimoto, S. Nishikawa, J. Imanishi, and S.-I. Nishikawa
Involvement of Runx1 in the down-regulation of fetal liver kinase-1 expression during transition of endothelial cells to hematopoietic cells
Blood,
September 15, 2005;
106(6):
1948 - 1955.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y. Bertrand, S. Giroux, R. Golub, M. Klaine, A. Jalil, L. Boucontet, I. Godin, and A. Cumano
Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin
PNAS,
January 4, 2005;
102(1):
134 - 139.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Gottgens, C. Broccardo, M.-J. Sanchez, S. Deveaux, G. Murphy, J. R. Gothert, E. Kotsopoulou, S. Kinston, L. Delaney, S. Piltz, et al.
The scl +18/19 Stem Cell Enhancer Is Not Required for Hematopoiesis: Identification of a 5' Bifunctional Hematopoietic-Endothelial Enhancer Bound by Fli-1 and Elf-1
Mol. Cell. Biol.,
March 1, 2004;
24(5):
1870 - 1883.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Alvarez-Silva, P. Belo-Diabangouaya, J. Salaun, and F. Dieterlen-Lievre
Mouse placenta is a major hematopoietic organ
Development,
November 15, 2003;
130(22):
5437 - 5444.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. S. Sequeira Lopez, D. R. Chernavvsky, T. Nomasa, L. Wall, M. Yanagisawa, and R. A. Gomez
The embryo makes red blood cell progenitors in every tissue simultaneously with blood vessel morphogenesis
Am J Physiol Regulatory Integrative Comp Physiol,
April 1, 2003;
284(4):
R1126 - R1137.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Hirashima, M. Ogawa, S. Nishikawa, K. Matsumura, K. Kawasaki, M. Shibuya, and S.-I. Nishikawa
A chemically defined culture of VEGFR2+ cells derived from embryonic stem cells reveals the role of VEGFR1 in tuning the threshold for VEGF in developing endothelial cells
Blood,
March 15, 2003;
101(6):
2261 - 2267.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Byrd, S. Becker, P. Maye, R. Narasimhaiah, B. St-Jacques, X. Zhang, J. McMahon, A. McMahon, and L. Grabel
Hedgehog is required for murine yolk sac angiogenesis
Development,
March 3, 2003;
129(2):
361 - 372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. McGrath, A. D. Koniski, J. Malik, and J. Palis
Circulation is established in a stepwise pattern in the mammalian embryo
Blood,
March 1, 2003;
101(5):
1669 - 1675.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Acarregui, K. M. England, J. T. Richman, and J. L. Littig
Characterization of CD34+ cells isolated from human fetal lung
Am J Physiol Lung Cell Mol Physiol,
February 1, 2003;
284(2):
L395 - L401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hirai, M. Ogawa, N. Suzuki, M. Yamamoto, G. Breier, O. Mazda, J. Imanishi, and S.-I. Nishikawa
Hemogenic and nonhemogenic endothelium can be distinguished by the activity of fetal liver kinase (Flk)-1 promoter/enhancer during mouse embryogenesis
Blood,
February 1, 2003;
101(3):
886 - 893.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Oberlin, M. Tavian, I. Blazsek, and B. Peault
Blood-forming potential of vascular endothelium in the human embryo
Development,
September 1, 2002;
129(17):
4147 - 4157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Sood, M. S. Fletcher, and M. J. C. Hendrix
The Embryonic-Like Properties of Aggressive Human Tumor Cells
Reproductive Sciences,
January 1, 2002;
9(1):
2 - 9.
[Abstract]
[PDF]
|
|
|
|
|
|
|
O. Ohneda, K. Ohneda, F. Arai, J. Lee, T. Miyamoto, Y. Fukushima, D. Dowbenko, L. A. Lasky, and T. Suda
ALCAM (CD166): its role in hematopoietic and endothelial development
Blood,
October 1, 2001;
98(7):
2134 - 2142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. DiMartino, L. Selleri, D. Traver, M. T. Firpo, J. Rhee, R. Warnke, S. O'Gorman, I. L. Weissman, and M. L. Cleary
The Hox cofactor and proto-oncogene Pbx1 is required for maintenance of definitive hematopoiesis in the fetal liver
Blood,
August 1, 2001;
98(3):
618 - 626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ausoni and S. Sartore
Cell Lineages and Tissue Boundaries in Cardiac Arterial and Venous Poles : Developmental Patterns, Animal Models, and Implications for Congenital Vascular Diseases
Arterioscler Thromb Vasc Biol,
March 1, 2001;
21(3):
312 - 320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Marshall, C. Kinnon, and A. J. Thrasher
Polarized expression of bone morphogenetic protein-4 in the human aorta-gonad-mesonephros region
Blood,
August 15, 2000;
96(4):
1591 - 1593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Uchida, M. Nakashima, Y. Hirota, Y. Miyazaki, T. Tsukazaki, and H. Shindo
Immunohistochemical localisation of protein tyrosine kinase receptors Tie-1 and Tie-2 in synovial tissue of rheumatoid arthritis: correlation with angiogenesis and synovial proliferation
Ann Rheum Dis,
August 1, 2000;
59(8):
607 - 614.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. J. Drake and P. A. Fleming
Vasculogenesis in the day 6.5 to 9.5 mouse embryo
Blood,
March 1, 2000;
95(5):
1671 - 1679.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A Manaia, V Lemarchandel, M Klaine, I Max-Audit, P Romeo, F Dieterlen-Lievre, and I Godin
Lmo2 and GATA-3 associated expression in intraembryonic hemogenic sites
Development,
January 2, 2000;
127(3):
643 - 653.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. G. Elefanty, C. G. Begley, L. Hartley, B. Papaevangeliou, and L. Robb
SCL Expression in the Mouse Embryo Detected With a Targeted lacZ Reporter Gene Demonstrates Its Localization to Hematopoietic, Vascular, and Neural Tissues
Blood,
December 1, 1999;
94(11):
3754 - 3763.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Crisa, V. Cirulli, K. A. Smith, M. H. Ellisman, B. E. Torbett, and D. R. Salomon
Human Cord Blood Progenitors Sustain Thymic T-Cell Development and a Novel Form of Angiogenesis
Blood,
December 1, 1999;
94(11):
3928 - 3940.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Delassus, I. Titley, and T. Enver
Functional and Molecular Analysis of Hematopoietic Progenitors Derived From the Aorta-Gonad-Mesonephros Region of the Mouse Embryo
Blood,
September 1, 1999;
94(5):
1495 - 1503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Godin, J. A. Garcia-Porrero, F. Dieterlen-Lievre, and A. Cumano
Stem Cell Emergence and Hemopoietic Activity Are Incompatible in Mouse Intraembryonic Sites
J. Exp. Med.,
July 1, 1999;
190(1):
43 - 52.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Begley and A. R. Green
The SCL Gene: From Case Report to Critical Hematopoietic Regulator
Blood,
May 1, 1999;
93(9):
2760 - 2770.
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Hamaguchi, X.-L. Huang, N. Takakura, J.-i. Tada, Y. Yamaguchi, H. Kodama, and T. Suda
In Vitro Hematopoietic and Endothelial Cell Development From Cells Expressing TEK Receptor in Murine Aorta-Gonad-Mesonephros Region
Blood,
March 1, 1999;
93(5):
1549 - 1556.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Cortes, F. Deschaseaux, N. Uchida, M.-C. Labastie, A. M. Friera, D. He, P. Charbord, and B. Peault
HCA, an Immunoglobulin-Like Adhesion Molecule Present on the Earliest Human Hematopoietic Precursor Cells, Is Also Expressed by Stromal Cells in Blood-Forming Tissues
Blood,
February 1, 1999;
93(3):
826 - 837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M Sanchez, B Gottgens, A. Sinclair, M Stanley, C. Begley, S Hunter, and A. Green
An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors
Development,
January 9, 1999;
126(17):
3891 - 3904.
[Abstract]
[PDF]
|
|
|
|
|
|
|
T North, T. Gu, T Stacy, Q Wang, L Howard, M Binder, M Marin-Padilla, and N. Speck
Cbfa2 is required for the formation of intra-aortic hematopoietic clusters
Development,
January 6, 1999;
126(11):
2563 - 2575.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L Pardanaud and F Dieterlen-Lievre
Manipulation of the angiopoietic/hemangiopoietic commitment in the avian embryo
Development,
January 2, 1999;
126(4):
617 - 627.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M.-C. Labastie, F. Cortes, P.-H. Romeo, C. Dulac, and B. Peault
Molecular Identity of Hematopoietic Precursor Cells Emerging in the Human Embryo
Blood,
November 15, 1998;
92(10):
3624 - 3635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Ohneda, C. Fennie, Z. Zheng, C. Donahue, H. La, R. Villacorta, B. Cairns, and L. A. Lasky
Hematopoietic Stem Cell Maintenance and Differentiation Are Supported by Embryonic Aorta-Gonad-Mesonephros Region-Derived Endothelium
Blood,
August 1, 1998;
92(3):
908 - 919.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. Liao, B. H. Paw, A. C. Oates, S. J. Pratt, J. H. Postlethwait, and L. I. Zon
SCL/Tal-1 transcription factor acts downstream of cloche to specify hematopoietic and vascular progenitors in zebrafish
Genes & Dev.,
March 1, 1998;
12(5):
621 - 626.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T Jaffredo, R Gautier, A Eichmann, and F Dieterlen-Lievre
Intraaortic hemopoietic cells are derived from endothelial cells during ontogeny
Development,
January 11, 1998;
125(22):
4575 - 4583.
[Abstract]
[PDF]
|
|
|
|
|
