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Further Analysis of Interleukin-2 Receptor Subunit Expression on the
Different Human Peripheral Blood Mononuclear Cell Subsets
Denis David,
Lynda Bani,
Jean-Louis Moreau,
Christophe Demaison,
Karine Sun,
Ombretta Salvucci,
Takayuki Nakarai,
Marianne de
Montalembert,
Salem Chouaïb,
Marcel Joussemet,
Jerome Ritz, and
Jacques Thèze
From the Unité d'Immunogénétique Cellulaire,
Département d'Immunologie, Institut Pasteur, Paris,
France; Laboratoire d'Immunologie, Institut Gustave Roussy, Villejuif,
France; Division of Hematologic Malignancies, Dana-Farber Cancer
Institute, Boston, MA; Site Transfusionnel de l'Hôpital
Necker-Enfants Malades, Paris, France; and Centre de Transfusion
Sanguine «Jean Julliard», Clamart, France.
We have investigated the expression of the three components of the
interleukin-2 receptor (IL-2R , IL-2R , and IL-2R ) on the
surface of the various peripheral blood mononuclear cell (PBMC) subsets
by flow cytometry analysis. The PBMC were immediately isolated (ficoll)
from blood collected on heparin as anticoagulant. The three IL-2R
components are absent or only marginally detectable on CD4 T
lymphocytes. No expression of the IL-2R chains is found for the B
lymphocytes. In most donors, the three chains are not detectable on CD8
T lymphocytes, but for a few of them, IL-2R or IL-2R are clearly
expressed. CD56 high (IL-2R+) and CD56 low
(IL-2R ) natural killer (NK) cells express IL-2R,
but not IL-2R. IL-2R is expressed by monocytes of all donors
although with variable intensity. When blood is collected on other
anticoagulants or when cells are isolated 1 day after collection,
IL-2R, IL-2R, and IL-2R are largely expressed on the surface
of most PBMC. This observation provides a possible explanation for
divergent data previously reported on IL-2R expression. Finally, we
show that IL-2R, which is not detectable on the cell surface of
lymphocytes, is nevertheless expressed and stored as an intracellular
component. This result is in agreement with the constitutive expression
of the IL-2R gene and suggests a specific regulatory mechanism for IL-2R membrane translocation.
Blood, Vol. 91 No. 1 (January 1), 1998:
pp. 165-172
© 1998 by The American Society of Hematology.
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