|
|
 Previous Article | Table of Contents | Next Article 
Lymphohematopoietic Engraftment in Minimally Myeloablated Hosts
F.M. Stewart,
S. Zhong,
J. Wuu,
C.-c. Hsieh,
S.K. Nilsson, and
P.J. Quesenberry
From the Cancer Center and Division of Hematology-Oncology,
University of Massachusetts Medical Center, Worcester, MA.
The concept that myeloablation to open space was a prerequisite for
marrow stem cell engraftment has been challenged by studies showing
high rates of engraftment in nonmyeloablated mice (Stewart et al,
Blood 81:2566, 1993; Quesenberry et al, Blood Cells
20:97, 1994; Brecher et al, Blood Cells 5:237, 1979; Saxe et
al, Exp Hematol 12:277, 1984; and Wu et al, Exp Hematol
21:251, 1993). However, relatively large numbers of marrow
cells were necessary to achieve high long-term donor percentages. We
have demonstrated, using a BALB/c male/female marrow transplant model
and detecting male DNA in host tissues by Southern blot or fluorescent
in situ hybridization, that exposure to doses of irradiation that cause minimal myeloablation (50 to 100 cGy) leads to very high levels of
donor chimerism, such that relatively small numbers of marrow cells (10 to 40 million) can give donor chimerism in the 40% to 100% range.
Studies of radiation sensitivity of long-term engrafting cells have
shown that 100 cGy, although not myelotoxic, is stem cell toxic, and
indicate that the final host:donor ratios are determined by competition
between host and donor stem cells. These data indicate that low levels
of irradiation should be an effective approach to nontoxic marrow
transplantation in gene therapy or in attempts to create allochimerism
to treat such diseases as cancer, sickle cell anemia, or thalassemia.
Blood, Vol. 91 No. 10 (May 15), 1998:
pp. 3681-3687
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. Bhattacharya, A. Czechowicz, A.G. L. Ooi, D. J. Rossi, D. Bryder, and I. L. Weissman
Niche recycling through division-independent egress of hematopoietic stem cells
J. Exp. Med.,
November 11, 2009;
(2009)
jem.20090778v3.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Z. Wang, G. Li, W. Tse, and K. D. Bunting
Conditional deletion of STAT5 in adult mouse hematopoietic stem cells causes loss of quiescence and permits efficient nonablative stem cell replacement
Blood,
May 14, 2009;
113(20):
4856 - 4865.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kumar and S. Ponnazhagan
Bone homing of mesenchymal stem cells by ectopic {alpha}4 integrin expression
FASEB J,
December 1, 2007;
21(14):
3917 - 3927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Chen, A. Larochelle, S. Fricker, G. Bridger, C. E. Dunbar, and J. L. Abkowitz
Mobilization as a preparative regimen for hematopoietic stem cell transplantation
Blood,
May 1, 2006;
107(9):
3764 - 3771.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Neff, B. C. Beard, and H.-P. Kiem
Survival of the fittest: in vivo selection and stem cell gene therapy
Blood,
March 1, 2006;
107(5):
1751 - 1760.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. O. Laukkanen, K. Kuramoto, B. Calmels, M. Takatoku, C. von Kalle, R. E. Donahue, and C. E. Dunbar
Low-dose total body irradiation causes clonal fluctuation of primate hematopoietic stem and progenitor cells
Blood,
February 1, 2005;
105(3):
1010 - 1015.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Richard, M. Weinreich, K.-H. Chang, J. Ieremia, M. M. Stevenson, and C. A. Blau
Modulating erythrocyte chimerism in a mouse model of pyruvate kinase deficiency
Blood,
June 15, 2004;
103(12):
4432 - 4439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Ito, Y. Takeuchi, J. Shaffer, and M. Sykes
Local irradiation enhances congenic donor pluripotent hematopoietic stem cell engraftment similarly in irradiated and nonirradiated sites
Blood,
March 1, 2004;
103(5):
1949 - 1954.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Abkowitz, A. E. Robinson, S. Kale, M. W. Long, and J. Chen
Mobilization of hematopoietic stem cells during homeostasis and after cytokine exposure
Blood,
August 15, 2003;
102(4):
1249 - 1253.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Montefusco, K. Ballen, and P. Quesenberry
Donor lymphocyte infusions as primary therapy for neoplasms
Blood,
January 1, 2003;
101(1):
373 - 373.
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. H. Peranteau, S. Hayashi, M. Hsieh, A. F. Shaaban, and A. W. Flake
High-level allogeneic chimerism achieved by prenatal tolerance induction and postnatal nonmyeloablative bone marrow transplantation
Blood,
August 28, 2002;
100(6):
2225 - 2234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. K. Ballen, P. S. Becker, R. V. B. Emmons, T. J. Fitzgerald, C. C. Hsieh, Q. Liu, C. Heyes, Y. Clark, W. Levy, J. F. Lambert, et al.
Low-dose total body irradiation followed by allogeneic lymphocyte infusion may induce remission in patients with refractory hematologic malignancy
Blood,
June 28, 2002;
100(2):
442 - 450.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. J. K. Noach, A. Ausema, J. H. Dillingh, B. Dontje, E. Weersing, I. Akkerman, E. Vellenga, and G. de Haan
Growth factor treatment prior to low-dose total body irradiation increases donor cell engraftment after bone marrow transplantation in mice
Blood,
June 17, 2002;
100(1):
312 - 317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. M. Stewart, S. Zhong, J.-F. Lambert, G. A. Colvin, M. Abedi, M. S. Dooner, C. I. McAuliffe, H. Wang, C. Hsieh, and P. J. Quesenberry
Host marrow stem cell potential and engraftability at varying times after low-dose whole-body irradiation
Blood,
August 15, 2001;
98(4):
1246 - 1251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bornhauser, C. Thiede, U. Platzbecker, A. Jenke, A. Helwig, R. Plettig, J. Freiberg-Richter, C. Rollig, G. Geissler, K. Lutterbeck, et al.
Dose-reduced Conditioning and Allogeneic Hematopoietic Stem Cell Transplantation from Unrelated Donors in 42 Patients
Clin. Cancer Res.,
August 1, 2001;
7(8):
2254 - 2262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Quesenberry, S. Zhong, H. Wang, and M. Stewart
Allogeneic chimerism with low-dose irradiation, antigen presensitization, and costimulator blockade in H-2 mismatched mice
Blood,
January 15, 2001;
97(2):
557 - 564.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. J. McCarthy and M. R. Bishop
Nonmyeloablative Allogeneic Stem Cell Transplantation: Early Promise and Limitations
Oncologist,
December 1, 2000;
5(6):
487 - 496.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. W. Flake and E. D. Zanjani
In Utero Hematopoietic Stem Cell Transplantation: Ontogenic Opportunities and Biologic Barriers
Blood,
October 1, 1999;
94(7):
2179 - 2191.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Quesenberry and P. S. Becker
Stem cell homing: Rolling, crawling, and nesting
PNAS,
December 22, 1998;
95(26):
15155 - 15157.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Parkman
The Future of Placental-Blood Transplantation
N. Engl. J. Med.,
November 26, 1998;
339(22):
1628 - 1629.
[Full Text]
|
|
|
|
|
