|
|
 Previous Article | Table of Contents | Next Article 
NF- B Transcription Factors Are Involved in Normal Erythropoiesis
Min-Ying Zhang,
Shao-Cong Sun,
Laurie Bell, and
Barbara A. Miller
From the Departments of Pediatrics and Microbiology and Immunology,
The Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey, PA.
NF- B/Rel designates a widely distributed family of transcription
factors involved in immune and acute phase responses. Here, the
expression and function of NF-B factors in erythroid proliferation and differentiation were explored. In an erythroleukemia cell line,
TF-1, high levels of p105/p50, p100/p52, p65, and IB were detected 24 hours after growth factor deprivation. In response to
granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulation, significant induction of p52 expression was observed. GM-CSF also induced nuclear translocation of both p52 and p65. No induction of
NF-B factors was observed with erythropoietin stimulation of TF-1
cells. Overexpression of p52 and p65 in TF-1 cells by transient
transfection resulted in significant induction of a B-TATA-luciferase reporter plasmid, showing that these factors are
functional in vivo in erythroid cells. To determine whether NF-B
factors may play a role in normal erythropoiesis, levels of these
factors were determined in burst-forming unit-erythroid (BFU-E)-derived cells at different stages of differentiation. The
NF-B factors p105/p50, p100/p52, and p65 were highly expressed in
early BFU-E-derived precursors, which are rapidly proliferating, and
declined during maturation. Furthermore, nuclear levels of NF-B
factors p50, p52, and p65 were higher in less mature precursors (day 10 BFU-E-derived cells) compared with more differentiated (day 14)
erythroblasts. In nuclear extracts from day 10 BFU-E-derived cells,
p50, p52, and p65 were able to form complexes, which bound to B
sites in the promoters of both the c-myb and c-myc
genes, suggesting that c-myb and c-myc may be among the B-containing genes regulated by NF-B factors in normal erythroid cells. Taken together, these data show that NF-B factors are modulated by GM-CSF
and suggest they function to regulate specific B containing genes
involved in erythropoiesis.
Blood, Vol. 91 No. 11 (June 1), 1998:
pp. 4136-4144
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. Bugarski, A. Krstic, S. Mojsilovic, M. Vlaski, M. Petakov, G. Jovcic, N. Stojanovic, and P. Milenkovic
Signaling Pathways Implicated in Hematopoietic Progenitor Cell Proliferation and Differentiation
Experimental Biology and Medicine,
January 1, 2007;
232(1):
156 - 163.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nakata, I. Matsumura, H. Tanaka, S. Ezoe, Y. Satoh, J. Ishikawa, T. Era, and Y. Kanakura
NF-{kappa}B Family Proteins Participate in Multiple Steps of Hematopoiesis through Elimination of Reactive Oxygen Species
J. Biol. Chem.,
December 31, 2004;
279(53):
55578 - 55586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Shojaei, L. Gallacher, and M. Bhatia
Differential gene expression of human stem progenitor cells derived from early stages of in utero human hematopoiesis
Blood,
April 1, 2004;
103(7):
2530 - 2540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ebner, A. Bandion, B. R. Binder, R. de Martin, and J. A. Schmid
GMCSF activates NF-{kappa}B via direct interaction of the GMCSF receptor with I{kappa}B kinase {beta}
Blood,
July 1, 2003;
102(1):
192 - 199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-J. Liu, S.-C. Hou, and C.-K. J. Shen
Erythroid Gene Suppression by NF-{kappa}B
J. Biol. Chem.,
May 23, 2003;
278(21):
19534 - 19540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Giron-Michel, M. Fogli, A. Gaggero, S. Ferrini, A. Caignard, D. Brouty-Boye, S. Baouz, M.-C. Le Bousse-Kerdiles, B. Peault, M. van Dijk, et al.
Detection of a Functional Hybrid Receptor {gamma}c/GM-CSFR{beta} in Human Hematopoietic CD34+ Cells
J. Exp. Med.,
March 17, 2003;
197(6):
763 - 775.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kirito, T. Watanabe, K.-i. Sawada, H. Endo, K. Ozawa, and N. Komatsu
Thrombopoietin Regulates Bcl-xL Gene Expression through Stat5 and Phosphatidylinositol 3-Kinase Activation Pathways
J. Biol. Chem.,
March 1, 2002;
277(10):
8329 - 8337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Shingo, S. T. Sorokan, T. Shimazaki, and S. Weiss
Erythropoietin Regulates the In Vitro and In Vivo Production of Neuronal Progenitors by Mammalian Forebrain Neural Stem Cells
J. Neurosci.,
December 15, 2001;
21(24):
9733 - 9743.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kawashima, K. Murata, S. Akira, Y. Tonozuka, Y. Minoshima, S. Feng, H. Kumagai, H. Tsuruga, Y. Ikeda, S. Asano, et al.
STAT5 Induces Macrophage Differentiation of M1 Leukemia Cells Through Activation of IL-6 Production Mediated by NF-{kappa}B p65
J. Immunol.,
October 1, 2001;
167(7):
3652 - 3660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Miller, D. L. Barber, L. L. Bell, B. K. Beattie, M.-Y. Zhang, B. G. Neel, M. Yoakim, L. I. Rothblum, and J. Y. Cheung
Identification of the Erythropoietin Receptor Domain Required for Calcium Channel Activation
J. Biol. Chem.,
July 16, 1999;
274(29):
20465 - 20472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. von Lindern, W. Zauner, G. Mellitzer, P. Steinlein, G. Fritsch, K. Huber, B. Lowenberg, and H. Beug
The Glucocorticoid Receptor Cooperates With the Erythropoietin Receptor and c-Kit to Enhance and Sustain Proliferation of Erythroid Progenitors In Vitro
Blood,
July 15, 1999;
94(2):
550 - 559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Pyatt, W. S. Stillman, Y. Yang, S. Gross, J. h. Zheng, and R. D. Irons
An Essential Role for NF-{kappa}B in Human CD34+ Bone Marrow Cell Survival
Blood,
May 15, 1999;
93(10):
3302 - 3308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-Y. Zhang, E. W. Harhaj, L. Bell, S.-C. Sun, and B. A. Miller
Bcl-3 Expression and Nuclear Translocation Are Induced by Granulocyte-Macrophage Colony-Stimulating Factor and Erythropoietin in Proliferating Human Erythroid Precursors
Blood,
August 15, 1998;
92(4):
1225 - 1234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
