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A New Cytokine-Dependent Monoblastic Cell Line With t(9;11)(p22;q23)
Differentiates to Macrophages With Macrophage Colony-Stimulating Factor
(M-CSF) and to Osteoclast-Like Cells With M-CSF and Interleukin-4
Takashi Ikeda,
Kazunori Sasaki,
Kazuma Ikeda,
Genji Yamaoka,
Koichi Kawanishi,
Yasunori Kawachi,
Tatsumi Uchida,
Jiro Takahara, and
Shozo Irino
From the First Department of Internal Medicine, the Department of
Transfusion Medicine, and the Department of Clinical Laboratory
Medicine, Kagawa Medical University, Kagawa, Japan; and the Department
of Internal Medicine, Takamatsu Red Cross Hospital, Kagawa, Japan.
Monocytes/macrophages exert a series of important functions in vivo.
To facilitate detailed investigation of their functional capacity and
the mechanism leading to their differentiation, several cell lines have
been established from primary material. We present here a new human
monoblastic cell line, designated UG3. UG3 cells are characterized by
the following features. (1) UG3 cells harbor the t(9;11)(p22;q23)
translocation that results in fusion of the MLL and the AF9 genes and
produce the corresponding AF9-MLL and MLL-AF9 fusion transcripts. (2)
UG3 cells rely on the presence of exogenous growth factors for
viability and proliferation, such as interleukin-3 (IL-3),
granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte
colony-stimulating factor (G-CSF), or macrophage colony-stimulating
factor (M-CSF). (3) When cultured in the presence of G-CSF, UG3 cells
differentiate along the granulocytic lineage, as evidenced by
segmentation of nuclei and positive staining for neutrophilic alkaline
phosphatase and peroxidase. (4) When cultured in the presence of GM-CSF
or M-CSF, UG3 cells differentiate into mature macrophages while
preserving surface expression of CD14 and CD68 and also start to
release cytokines into cell-culture supernatants. Under these culture
conditions, UG3 cells also take up acetylated LDL. (5) When cultured in
the presence of M-CSF and IL-4, UG3 cells differentiate into
osteoclast-like multinucleated giant cells capable of bone resorption
and display tartrate-resistant acid phosphatase (TRAP) activity. UG3
cells thus provide features to qualify them as a useful model to
further investigate the mechanism underlying these processes and also
to further elucidate the functional role of mature
monocytes/macrophages or osteoclasts.
Blood, Vol. 91 No. 12 (June 15), 1998:
pp. 4543-4553
© 1998 by The American Society of Hematology.
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