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Characterization of t(2;5) Reciprocal Transcripts and Genomic
Breakpoints in CD30+ Cutaneous Lymphoproliferations
M. Beylot-Barry,
A. Groppi,
B. Vergier,
K. Pulford, and
J.P. Merlio for the French Study Group of Cutaneous Lymphoma
From CHU of Bordeaux and University of Bordeaux II, Bordeaux, France;
LRF Immunodiagnostics Unit, John Radcliffe Hospital; and the French
Study Group of Cutaneous Lymphoma, Créteil, France.
NPM-ALK chimeric transcripts, encoded by the t(2;5), lead to an
aberrant expression of ALK by CD30+ systemic lymphomas.
To determine if t(2;5) is involved in cutaneous lymphoproliferative
disorders, we studied 37 CD30+ cutaneous
lymphoproliferations, 27 mycosis fungoides (MF), and 16 benign
inflammatory disorders (BID). NPM-ALK transcripts were detected by
nested reverse transcription-polymerase chain reaction (RT-PCR) in 1 of 11 lymphomatoid papulosis (LyP), 7 of
15 CD30+ primary cutaneous T-cell lymphoma (CTCL), 3 of
11 CD30+ secondary cutaneous lymphoma, 6 of 27 MF, and 1 of 16 BID. However, the expression of NPM-ALK transcripts was not
associated with ALK1 immunoreactivity in MF, LyP, or BID cases. Only 1 CD30+ primary CTCL and 3 CD30+ secondary
cutaneous lymphoma were ALK1 immunoreactive. The ALK1+
cases were also characterized by amplification of tumor-specific genomic breakpoints on derivative chromosome 5. These cases, except for
1 secondary cutaneous lymphoma, were also characterized by reciprocal
breakpoints on derivative chromosome 2, leading to the expression of
reciprocal ALK-NPM transcripts. Amplification of chromosomal
breakpoints on both derivative chromosomes could represent an
alternative to conventional cytogenetics for the diagnosis of t(2;5)
and seems to be more reliable than the detection of cryptic NPM-ALK
transcripts by nested RT-PCR.
Blood, Vol. 91 No. 12 (June 15), 1998:
pp. 4668-4676
© 1998 by The American Society of Hematology.
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