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The Bactericidal/Permeability-Increasing Protein (BPI) Is Present
in Specific Granules of Human Eosinophils
Jero Calafat,
Hans Janssen,
Anton Tool,
Mieke A. Dentener,
Edward
F. Knol,
Helene F. Rosenberg, and
Arne Egesten
From the Division of Cell Biology, The Netherlands Cancer Institute,
Amsterdam, The Netherlands; the Central Laboratory of The Netherlands
Red Cross Blood Transfusion Service, Amsterdam, The Netherlands; the
Department of Pulmonology, Maastricht University, Maastricht, The
Netherlands; the Laboratory of Host Defenses, NIAID, National
Institutes of Health, Bethesda, MD; and the Department of Medicine,
Lund University, University Hospital MAS, Malmö, Sweden.
Eosinophils participate in the inflammatory response seen in allergy
and parasitic infestation, but a role in host defense against bacterial
infection is not settled. The bactericidal/permeability-increasing protein (BPI) has been demonstrated in neutrophils and it exerts bacteriostatic and bactericidal effects against a wide variety of
Gram-negative bacterial species. Using the Western blot technique, a
55-kD band, corresponding to BPI, was detected in lysates from both
neutrophils and eosinophils. The localization of BPI in immature and
mature eosinophils was investigated using immunoelectron microscopy. BPI was found in immature and mature specific granules of eosinophils and was detected in phagosomes as well, indicating release of the
protein from the granules into the phagosomes. Using a specific enzyme-linked immunosorbent assay, eosinophils were shown to contain 179 ng of BPI/5 × 106 eosinophils compared with 710 ng
BPI/5 × 106 neutrophils. The presence of BPI in
eosinophils suggests a role for these cells in host defense against
Gram-negative bacterial invasion or may suggest a role for BPI against
parasitic infestation.
Blood, Vol. 91 No. 12 (June 15), 1998:
pp. 4770-4775
© 1998 by The American Society of Hematology.
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