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High Efficiency Adenovirus-Mediated Gene Transfer to Human Dendritic
Cells
Allan B. Dietz and
Stanimir Vuk-Pavlovi
From the Stem Cell Laboratory, Mayo Cancer Center, Mayo Clinic and
Mayo Foundation, Rochester, MN.
The interest in the use of human dendritic cells in cancer
immunotherapy calls for efficient ex vivo methods of dendritic cell
education. To extend the range of methods available, we generated phenotypically characteristic dendritic cells from peripheral blood
monocytes incubated with granulocyte-macrophage colony-stimulating factor and interleukin-4 and infected them with an adenovirus containing a humanized version of green fluorescent protein as a marker
of gene expression. The levels of expressed protein were high, but they
were further increased in combination with cationic liposomes. In
comparison to transfection efficiency of the homologous expression
plasmid, adenovirus-mediated gene transfer was substantially more
efficient. With the aid of liposome-mediated infection, gene transfer
into CD83+ dendritic cells was highly effective,
resulting in more than 90% of the cells expressing the transgene.
Blood, Vol. 91 No. 2 (January 15), 1998:
pp. 392-398
© 1998 by The American Society of Hematology.
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