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Thrombopoietin and Its Receptor, c-mpl, Are Constitutively
Expressed by Mouse Liver Endothelial Cells: Evidence of
Thrombopoietin as a Growth Factor for Liver Endothelial Cells
José E. Cardier and
Jack Dempsey
From the Hipple Cancer Research Center, Dayton, OH.
Present data suggest that the primary site of thrombopoietin (TPO)
mRNA is the liver. Previously, we reported that specific murine liver
endothelial cells (LEC-1) located in the hepatic sinusoids support in
vitro megakaryocytopoiesis from murine hematopoietic stem cells
suggesting that these cells may be a source of TPO. We report here that
TPO and its receptor, c-mpl, are coexpressed on cloned LEC-1.
Enzyme-linked immunosorbent assay (ELISA), biological assay, and flow
cytometry studies confirmed the expression of both TPO and its
receptor, respectively, at the protein level. TPO activity was enhanced
in supernatants from LEC-1 treated with tumor necrosis factor (TNF)-
and  -interferon (INF). Our results show that TPO through its
receptor stimulated the growth of LEC-1 in vitro. These observations
establish LEC-1 as a novel source of TPO in the liver. To our
knowledge, this is the first report that liver endothelial cells
express both TPO and its receptor, c-mpl, and our findings
indicate that this cytokine constitutes a growth factor for liver
endothelial cells in vitro.
Blood, Vol. 91 No. 3 (February 1), 1998:
pp. 923-929
© 1998 by The American Society of Hematology.
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