|
|
 Previous Article | Table of Contents | Next Article 
Transmigration of CD34+ Cells Across Specialized and
Nonspecialized Endothelium Requires Prior Activation by Growth
Factors and Is Mediated by PECAM-1 (CD31)
Kwee L. Yong,
Mike Watts,
N. Shaun Thomas,
Angela Sullivan,
Stuart Ings, and
David C. Linch
From the Department of Haematology, Royal Free Hospital School of
Medicine, London, UK and the Department of Haematology, University
College Medical School, London, UK.
The transmigration of hematopoietic progenitor cells (HPCs) across
vascular endothelium is a critical step in the homing of transplanted
stem cells, but the molecular basis for this is unknown. We used
mobilized peripheral blood CD34+ selected cells and
cultured bone marrow microvascular (BMECs) and human umbilical vein
endothelial cells (HUVECs) to investigate the adhesion and
transendothelial migration of HPCs. Colony-forming cells (CFCs) in
freshly isolated CD34+ cells showed high levels of
adhesion to both forms of endothelium (28% ± 4% and 38% ± 6% of
granulocyte-macrophage colony-forming cells [GM-CFCs] adhering to
HUVECs and BMECs, respectively), but were unable to migrate to any
significant extent across either (1.0% ± 0.3% and 1.1% ± 0.6%
of GM-CFCs migrating across HUVECs and BMECs, respectively). Greater
than 95% of peripheral blood CD34+ cells are in
G0/G1 of the cell cycle, but after 48 to 72 hours of stimulation with growth factors (interleukin-3 [IL-3] 12 ng/mL, stem cell factor 10 ng/mL, and IL-6 10 ng/mL), 28% ± 5% of
cells were in S+G2/M. Growth factor stimulation had no
effect on the adhesion of mobilized CFCs but resulted in enhanced
migration of these cells (9.8% ± 1.6% and 12.6% ± 3.1% of
GM-CFCs migrating across HUVECs and BMECs, respectively; P < .01, n = 6). Assessment of cell proliferation by the
3H-thymidine suicide method showed that, whereas 11.7% ± 3.3% of proliferating CFCs transmigrated across endothelium, only
1.3% ± 0.3% of nonproliferating CFCs did so (P < .05, n
= 5). Transmigration of growth factor-activated CFCs was inhibited by
anti-CD18 monoclonal antibody (MoAb; 50% ± 18% inhibition) and by
anti-platelet endothelial cell adhesion molecule-1 (PECAM-1) MoAb
(70.8% ± 7.1% inhibition; P < .05, n = 3). IL-1
stimulation of HUVECs had no significant effect on CD34+
cell transmigration, but caused marked enhancement of neutrophil migration. Stem cell homing may depend, in part, on the ability of
local cytokines to upregulate the transmigratory ability of these
cells. The transmigration of HPCs shares at least some molecular pathways with that of mature cells (CD18 and PECAM-1), but
is differently affected by endothelial activation.
Blood, Vol. 91 No. 4 (February 15), 1998:
pp. 1196-1205
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
N. Gallay, L. Anani, A. Lopez, P. Colombat, C. Binet, J. Domenech, B. B. Weksler, F. Malavasi, and O. Herault
The Role of Platelet/Endothelial Cell Adhesion Molecule 1 (CD31) and CD38 Antigens in Marrow Microenvironmental Retention of Acute Myelogenous Leukemia Cells
Cancer Res.,
September 15, 2007;
67(18):
8624 - 8632.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-M. Imbert, G. Belaaloui, F. Bardin, C. Tonnelle, M. Lopez, and C. Chabannon
CD99 expressed on human mobilized peripheral blood CD34+ cells is involved in transendothelial migration
Blood,
October 15, 2006;
108(8):
2578 - 2586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. F. M. Segers, I. Van Riet, L. J. Andries, K. Lemmens, M. J. Demolder, A. J. M. L. De Becker, M. M. Kockx, and G. W. De Keulenaer
Mesenchymal stem cell adhesion to cardiac microvascular endothelium: activators and mechanisms
Am J Physiol Heart Circ Physiol,
April 1, 2006;
290(4):
H1370 - H1377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Pritsker, T. T. Doniger, L. C. Kramer, S. E. Westcot, and I. R. Lemischka
Diversification of stem cell molecular repertoire by alternative splicing
PNAS,
October 4, 2005;
102(40):
14290 - 14295.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-G. Kopp, S. T. Avecilla, A. T. Hooper, and S. Rafii
The Bone Marrow Vascular Niche: Home of HSC Differentiation and Mobilization
Physiology,
October 1, 2005;
20(5):
349 - 356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Ahmed, S. J. Ings, A. R. Pizzey, M. P. Blundell, A. J. Thrasher, H. T. Ye, A. Fahey, D. C. Linch, and K. L. Yong
Impaired bone marrow homing of cytokine-activated CD34+ cells in the NOD/SCID model
Blood,
March 15, 2004;
103(6):
2079 - 2087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Scott, G. V. Priestley, and T. Papayannopoulou
Deletion of {alpha}4 Integrins from Adult Hematopoietic Cells Reveals Roles in Homeostasis, Regeneration, and Homing
Mol. Cell. Biol.,
December 15, 2003;
23(24):
9349 - 9360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Melani, C. Chiodoni, G. Forni, and M. P. Colombo
Myeloid cell expansion elicited by the progression of spontaneous mammary carcinomas in c-erbB-2 transgenic BALB/c mice suppresses immune reactivity
Blood,
September 15, 2003;
102(6):
2138 - 2145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Newman and D. K. Newman
Signal Transduction Pathways Mediated by PECAM-1: New Roles for an Old Molecule in Platelet and Vascular Cell Biology
Arterioscler. Thromb. Vasc. Biol.,
June 1, 2003;
23(6):
953 - 964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Denning-Kendall, S. Singha, B. Bradley, and J. Hows
Cytokine Expansion Culture of Cord Blood CD34+ Cells Induces Marked and Sustained Changes in Adhesion Receptor and CXCR4 Expressions
Stem Cells,
January 1, 2003;
21(1):
61 - 70.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Ferrero, D. Belloni, P. Contini, C. Foglieni, M. E. Ferrero, M. Fabbri, A. Poggi, and M. R. Zocchi
Transendothelial migration leads to protection from starvation-induced apoptosis in CD34+CD14+ circulating precursors: evidence for PECAM-1 involvement through Akt/PKB activation
Blood,
January 1, 2003;
101(1):
186 - 193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. E. Burger, S. Coetzee, W. L. McKeehan, M. Kan, P. Cook, Y. Fan, T. Suda, R. P. Hebbel, N. Novitzky, W. A. Muller, et al.
Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells
Blood,
November 15, 2002;
100(10):
3527 - 3535.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Netelenbos, S. Zuijderduijn, J. van den Born, F. L. Kessler, S. Zweegman, P. C. Huijgens, and A. M. Drager
Proteoglycans guide SDF-1-induced migration of hematopoietic progenitor cells
J. Leukoc. Biol.,
August 1, 2002;
72(2):
353 - 362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Giet, D. R. Van Bockstaele, I. Di Stefano, S. Huygen, R. Greimers, Y. Beguin, and A. Gothot
Increased binding and defective migration across fibronectin of cycling hematopoietic progenitor cells
Blood,
March 15, 2002;
99(6):
2023 - 2031.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Wiewrodt, A. P. Thomas, L. Cipelletti, M. Christofidou-Solomidou, D. A. Weitz, S. I. Feinstein, D. Schaffer, S. M. Albelda, M. Koval, and V. R. Muzykantov
Size-dependent intracellular immunotargeting of therapeutic cargoes into endothelial cells
Blood,
February 1, 2002;
99(3):
912 - 922.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-P. Levesque, Y. Takamatsu, S. K. Nilsson, D. N. Haylock, and P. J. Simmons
Vascular cell adhesion molecule-1 (CD106) is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor
Blood,
September 1, 2001;
98(5):
1289 - 1297.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Voermans, P.M.L. Rood, P.L. Hordijk, W.R. Gerritsen, and C.E. van der Schoot
Adhesion Molecules Involved in Transendothelial Migration of Human Hematopoietic Progenitor Cells
Stem Cells,
November 1, 2000;
18(6):
435 - 443.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. Riedl, J. Stockl, O. Majdic, C. Scheinecker, K. Rappersberger, W. Knapp, and H. Strobl
Functional Involvement of E-Cadherin in TGF-{beta}1-Induced Cell Cluster Formation of In Vitro Developing Human Langerhans-Type Dendritic Cells
J. Immunol.,
August 1, 2000;
165(3):
1381 - 1386.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Mahooti, D. Graesser, S. Patil, P. Newman, G. Duncan, T. Mak, and J. A. Madri
PECAM-1 (CD31) Expression Modulates Bleeding Time in Vivo
Am. J. Pathol.,
July 1, 2000;
157(1):
75 - 81.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Peled, O. Kollet, T. Ponomaryov, I. Petit, S. Franitza, V. Grabovsky, M. M. Slav, A. Nagler, O. Lider, R. Alon, et al.
The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34+ cells: role in transendothelial/stromal migration and engraftment of NOD/SCID mice
Blood,
June 1, 2000;
95(11):
3289 - 3296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Naiyer, D.-Y. Jo, J. Ahn, R. Mohle, M. Peichev, G. Lam, R. L. Silverstein, M. A.S. Moore, and S. Rafii
Stromal Derived Factor-1-Induced Chemokinesis of Cord Blood CD34+ Cells (Long-Term Culture-Initiating Cells) Through Endothelial Cells Is Mediated by E-Selectin
Blood,
December 15, 1999;
94(12):
4011 - 4019.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Hordijk, E Anthony, F. Mul, R Rientsma, L. Oomen, and D Roos
Vascular-endothelial-cadherin modulates endothelial monolayer permeability
J. Cell Sci.,
January 6, 1999;
112(12):
1915 - 1923.
[Abstract]
[PDF]
|
|
|
|
|
|
|
K. Imai, M. Kobayashi, J. Wang, Y. Ohiro, J.-i. Hamada, Y. Cho, M. Imamura, M. Musashi, T. Kondo, M. Hosokawa, et al.
Selective Transendothelial Migration of Hematopoietic Progenitor Cells: A Role in Homing of Progenitor Cells
Blood,
January 1, 1999;
93(1):
149 - 156.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Quesenberry and P. S. Becker
Stem cell homing: Rolling, crawling, and nesting
PNAS,
December 22, 1998;
95(26):
15155 - 15157.
[Full Text]
[PDF]
|
|
|
|
|
