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The Relationship of Blood Lymphocytes to the Recirculating Lymphocyte
Pool
William N. Andrade,
Miles G. Johnston, and
John B. Hay
From the Departments of Pathology and Immunology, University of
Toronto, Toronto; and Trauma Research Program, Sunnybrook Health
Science Centre, North York, Ontario, Canada.
Lymphocyte recirculation facilitates the detection and elimination
of pathogens and the dissemination of immunologic memory. It is
generally assumed that all small lymphocytes in the blood are actively
recirculating, yet there is little quantitative data directly comparing
the migration of this population with actively recirculating,
lymph-derived lymphocytes. In this study blood lymphocytes were labeled
with fluorescein isothiocyanate (FITC), and lymph lymphocytes were
labeled with CM-DiI, reinfused intravenously, and monitored in blood
and lymph. After equilibration the concentration of blood lymphocytes
was several times higher in blood than in lymph, whereas lymph
lymphocytes displayed the opposite behavior. This suggested that blood
lymphocytes did not recirculate as efficiently as lymph lymphocytes, so
we examined the following blood lymphocyte subsets in greater detail: B
cells, CD4+, CD8+, and   T cells.
Within 4 hours postinjection the percentage of FITC+
CD8+ and CD4+ lymphocytes fell in the blood
and remained significantly lower than the injected sample. In contrast,
the concentration of FITC+ T cells did not change,
and the percentage of FITC+ B cells increased. These data
suggest that subpopulations of B and perhaps T lymphocytes in
the blood do not recirculate efficiently through lymph nodes.
Blood, Vol. 91 No. 5 (March 1), 1998:
pp. 1653-1661
© 1998 by The American Society of Hematology.
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