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Establishing a KSHV+ Cell Line (BCP-1) From Peripheral
Blood and Characterizing Its Growth in Nod/SCID Mice
Chris Boshoff,
Shou-Jiang Gao,
Lyn E. Healy,
Steve Matthews,
Alero
J. Thomas,
Loinel Coignet,
Roger A. Warnke,
James A. Strauchen,
Estella Matutes,
Onsi W. Kamel,
Patrick S. Moore,
Robin A. Weiss, and
Yuan Chang
From the Chester Beatty Laboratories, Institute of Cancer Research,
London, UK; the Department of Pathology and School of Public Health,
Columbia University, New York, NY; the Clinical Sciences Department,
London School of Hygiene and Tropical Medicine, University of London,
London, UK; the Department of Pathology, Stanford University School of
Medicine, Stanford, CA; and the Department of Pathology, Mount Sinai
School of Medicine, New York, NY.
Kaposi's sarcoma-associated herpesvirus (KSHV or HHV8) sequences
are present in primary effusion lymphomas (PEL). KSHV+
cell lines have been established from such lymphomas. Here we report
the first description of the establishment of a KSHV+,
EBV cell line (BCP-1) from the peripheral blood of a
patient with PEL. Using this cell line and a KSHV+,
EBV+ PEL cell line (HBL-6) previously established from
ascitic fluid, we investigated whether in nonobese diabetic/severe
combined immunodeficiency disease (Nod/SCID) mice tumors representing
PEL can be established. When injected intravenously (IV) into Nod/SCID
mice, BCP-1 and HBL-6 infiltrated organs, with only occasional
macroscopic tumor formation. Intraperitoneal injections (ip) led to the
development of ascites and diffuse infiltration of organs, without
obviously solid lymphoma formation, resembling the diffuse nature of
human PEL. To investigate a possible mechanism for the peculiar
phenotype of PEL, we examine the presence of adhesion molecules and
homing markers on PEL cells before and after growing in mice. Both
BCP-1 and HBL-6 cells lack expression of important cytoadhesion
molecules including CD11a and CD18 (LFA1  and  chains), CD29,
CD31, CD44, CD54 (ICAM-1), and CD62L and E (L and E selectins).
Blood, Vol. 91 No. 5 (March 1), 1998:
pp. 1671-1679
© 1998 by The American Society of Hematology.
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