|
|
 Previous Article | Table of Contents | Next Article 
In Vitro Incorporation of GPI-Anchored Proteins Into Human
Erythrocytes and Their Fate in the Membrane
Gianluca Civenni,
Samuel T. Test,
Urs Brodbeck, and
Peter Bütikofer
From the Institute of Biochemistry and Molecular Biology, the
University of Bern, Bern, Switzerland; and Children's Hospital Oakland
Research Institute, Oakland, CA.
In many different cells, glycosylphosphatidylinositol (GPI)-anchored
molecules are clustered in membrane microdomains that resist extraction
by detergents at 4°C. In this report, we identified the presence of
such domains in human erythrocytes and examined the ability of
exogenously-added GPI-anchored molecules to colocalize with the
endogenous GPI-anchored proteins in these detergent-insoluble complexes. We found that the addition to human erythrocytes of three
purified GPI-anchored proteins having different GPI lipid moieties
resulted in their efficient and correct incorporation into the
membrane. The extent of membrane insertion was dependent on the
intactness of the GPI lipid moiety. However, unlike the endogenous
GPI-anchored proteins, the in vitro incorporated GPI molecules were not
resistant to membrane extraction by Triton X-100 at 4°C.
In addition, in contrast to the endogenous GPI-anchored proteins, they
were not preferentially released from erythrocytes during vesiculation
induced by calcium loading of the cells. These results suggest that in
vitro incorporated GPI-linked molecules are excluded from pre-existing
GPI-enriched membrane areas in human erythrocytes and that these
microdomains may represent the sites of membrane vesicle formation.
Blood, Vol. 91 No. 5 (March 1), 1998:
pp. 1784-1792
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. M. Pasini, M. Kirkegaard, D. Salerno, P. Mortensen, M. Mann, and A. W. Thomas
Deep Coverage Mouse Red Blood Cell Proteome: A First Comparison with the Human Red Blood Cell
Mol. Cell. Proteomics,
July 1, 2008;
7(7):
1317 - 1330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Pasini, M. Kirkegaard, P. Mortensen, H. U. Lutz, A. W. Thomas, and M. Mann
In-depth analysis of the membrane and cytosolic proteome of red blood cells
Blood,
August 1, 2006;
108(3):
791 - 801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T.-y. Wang, R. Leventis, and J. R. Silvius
Artificially Lipid-anchored Proteins Can Elicit Clustering-induced Intracellular Signaling Events in Jurkat T-Lymphocytes Independent of Lipid Raft Association
J. Biol. Chem.,
June 17, 2005;
280(24):
22839 - 22846.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Sloand, L. Mainwaring, K. Keyvanfar, J. Chen, J. Maciejewski, H. G. Klein, and N. S. Young
Transfer of glycosylphosphatidylinositol-anchored proteins to deficient cells after erythrocyte transfusion in paroxysmal nocturnal hemoglobinuria
Blood,
December 1, 2004;
104(12):
3782 - 3788.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. C. Murphy, B. U. Samuel, T. Harrison, K. D. Speicher, D. W. Speicher, M. E. Reid, R. Prohaska, P. S. Low, M. J. Tanner, N. Mohandas, et al.
Erythrocyte detergent-resistant membrane proteins: their characterization and selective uptake during malarial infection
Blood,
March 1, 2004;
103(5):
1920 - 1928.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Kifor, I. Kifor, F. D. Moore Jr., R. R. Butters Jr., and E. M. Brown
m-Calpain Colocalizes with the Calcium-sensing Receptor (CaR) in Caveolae in Parathyroid Cells and Participates in Degradation of the CaR
J. Biol. Chem.,
August 15, 2003;
278(33):
31167 - 31176.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Salzer and R. Prohaska
Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts
Blood,
February 15, 2001;
97(4):
1141 - 1143.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Butikofer;, E. Sloand, J. Maciejewski, D. Dunn, J. Moss, and N. Young
Correction of the PNH defect by GPI protein transfer: still an open question
Blood,
March 1, 2000;
95(5):
1876 - 1877.
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Y. S. Chuang, M. Sassaroli, and J. C. Unkeless
Convergence of Fc{gamma} Receptor IIA and Fc{gamma} Receptor IIIB Signaling Pathways in Human Neutrophils
J. Immunol.,
January 1, 2000;
164(1):
350 - 360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. U. Samuel, N. Mohandas, T. Harrison, H. McManus, W. Rosse, M. Reid, and K. Haldar
The Role of Cholesterol and Glycosylphosphatidylinositol-anchored Proteins of Erythrocyte Rafts in Regulating Raft Protein Content and Malarial Infection
J. Biol. Chem.,
July 27, 2001;
276(31):
29319 - 29329.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
