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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Metes, D. Articles by Morel, P. A. Search for Related Content PubMed PubMed Citation Articles by Metes, D. Articles by Morel, P. A. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Expression of Functional CD32 Molecules on Human NK Cells Is Determined by an Allelic Polymorphism of the FcRIIC Gene Diana Metes, Linda K. Ernst, William H. Chambers, Andrei Sulica, Ronald B. Herberman, and Penelope A. Morel From the Departments of Pathology, Medicine, and Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine; the University of Pittsburgh Cancer Institute, Pittsburgh, PA; and the Center of Immunology, Institute of Virology, Bucharest, Romania. Human natural killer (NK) cells were thought to express only FcRIIIA (CD16), but recent reports have indicated that NK cells also express a second type of FcR, ie, FcRII (CD32). We have isolated, cloned, and sequenced full-length cDNAs of FcRII from NK cells derived from several normal individuals that may represent four different products of the FcRIIC gene. One transcript (IIc1) is identical with the already described FcRIIc form. The other three (IIc2-IIc4) appear to represent unique, alternatively spliced products of the same gene, and include a possible soluble form. Analyses of the full-length clones have revealed an allelic polymorphism in the first extracellular exon, resulting in either a functional open reading frame isoform or a null allele. Stable transfection experiments enabled us to determine a unique binding pattern of anti-CD32 monoclonal antibodies to FcRIIc. Further analyses of NK-cell preparations revealed heterogeneity in CD32 expression, ranging from donors lacking CD32 expression to donors expressing high levels of CD32 that were capable of triggering cytotoxicity. Differences in expression were correlated with the presence or absence of null alleles. These data show that certain individuals express high levels of functional FcRIIc isoforms on their NK cells. Blood, Vol. 91 No. 7 (April 1), 1998: pp. 2369-2380 © 1998 by The American Society of Hematology. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C.-A. Dutertre, E. Bonnin-Gelize, K. 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