|
|
 Previous Article | Table of Contents | Next Article 
Isolation and Characterization of the cDNA for Mouse Neutrophil
Collagenase: Demonstration of Shared Negative Regulatory Pathways for
Neutrophil Secondary Granule Protein Gene Expression
Nathan D. Lawson,
Arati Khanna-Gupta, and
Nancy Berliner
From the Department of Biology and Section of Hematology, Department
of Internal Medicine, Yale University School of Medicine, New Haven,
CT.
A characteristic of normal neutrophil maturation is the induction of
secondary granule protein (SGP) mRNA expression. Several leukemic human
cell lines mimic normal morphologic neutrophil differentiation but fail
to express SGPs, such as lactoferrin (LF) and neutrophil gelatinase
(NG). In contrast, two murine cell lines (32D C13 and MPRO) are able to
differentiate into neutrophils and induce expression of LF and NG.
Therefore, to study the normal regulation and function of these genes,
the corresponding murine homologs must be isolated. Using cDNA
representational difference analysis (RDA) to compare a committed
myeloid progenitor cell line (EPRO) with the multipotent stem cell line
from which it was derived (EML), we isolated a fragment bearing
homology to human neutrophil collagenase (hNC). Here, we describe the
cloning and characterization of a full-length (~2 kb) clone that
exhibits nearly 65% nucleotide and 73% amino acid identity to hNC.
Ribonuclease protection analysis (RPA) of the tissues and cell lines
shows that mouse NC (mNC) is expressed only in cell lines exhibiting neutrophilic characteristics, further confirming its identity as the
mouse homolog of hNC. Furthermore, we have demonstrated a shared
negative regulatory pathway for this and other SGP genes. We have
previously shown that CCAAT displacement protein (CDP/cut) binds to a
specific region of the LF promoter, and overexpression of CDP blocks
G-CSF-induced upregulation of LF gene expression in 32D C13
cells. We show here that in these cells, upregulation of both
NC and NG is also blocked. CDP is thus the first identified transcription factor that is a candidate for mediating the shared regulation of neutrophil SGP protein genes.
Blood, Vol. 91 No. 7 (April 1), 1998:
pp. 2517-2524
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
H. Wang, S. Lindsey, I. Konieczna, L. Bei, E. Horvath, W. Huang, G. Saberwal, and E. A. Eklund
Constitutively Active SHP2 Cooperates with HoxA10 Overexpression to Induce Acute Myeloid Leukemia
J. Biol. Chem.,
January 23, 2009;
284(4):
2549 - 2567.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Eliason, K. K. Hannawa, G. Ailawadi, I. Sinha, J. W. Ford, M. P. Deogracias, K. J. Roelofs, D. T. Woodrum, T. L. Ennis, P. K. Henke, et al.
Neutrophil Depletion Inhibits Experimental Abdominal Aortic Aneurysm Formation
Circulation,
July 12, 2005;
112(2):
232 - 240.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Gaines, J. Chi, and N. Berliner
Heterogeneity of functional responses in differentiated myeloid cell lines reveals EPRO cells as a valid model of murine neutrophil functional activation
J. Leukoc. Biol.,
May 1, 2005;
77(5):
669 - 679.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Maun, P. Gaines, A. Khanna-Gupta, T. Zibello, L. Enriquez, L. Goldberg, and N. Berliner
G-CSF signaling can differentiate promyelocytes expressing a defective retinoic acid receptor: evidence for divergent pathways regulating neutrophil differentiation
Blood,
March 1, 2004;
103(5):
1693 - 1701.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Truscott, L. Raynal, P. Premdas, B. Goulet, L. Leduy, G. Berube, and A. Nepveu
CDP/Cux Stimulates Transcription from the DNA Polymerase {alpha} Gene Promoter
Mol. Cell. Biol.,
April 15, 2003;
23(8):
3013 - 3028.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Goulet, P. Watson, M. Poirier, L. Leduy, G. Berube, S. Meterissian, P. Jolicoeur, and A. Nepveu
Characterization of a Tissue-specific CDP/Cux Isoform, p75, Activated in Breast Tumor Cells
Cancer Res.,
November 15, 2002;
62(22):
6625 - 6633.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Sinclair, J. A. Lee, A. Goldstein, D. Xing, S. Liu, R. Ju, P. W. Tucker, E. J. Neufeld, and R. H. Scheuermann
Lymphoid apoptosis and myeloid hyperplasia in CCAAT displacement protein mutant mice
Blood,
December 15, 2001;
98(13):
3658 - 3667.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Khanna-Gupta, T. Zibello, H. Sun, J. Lekstrom-Himes, and N. Berliner
C/EBPvarepsilon mediates myeloid differentiation and is regulated by the CCAAT displacement protein (CDP/cut)
PNAS,
July 3, 2001;
98(14):
8000 - 8005.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Khanna-Gupta, T. Zibello, C. Simkevich, A. G. Rosmarin, and N. Berliner
Sp1 and C/EBP are necessary to activate the lactoferrin gene promoter during myeloid differentiation
Blood,
June 15, 2000;
95(12):
3734 - 3741.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. R. Calvo, D. B. Sykes, M. Pasillas, and M. P. Kamps
Hoxa9 Immortalizes a Granulocyte-Macrophage Colony-Stimulating Factor-Dependent Promyelocyte Capable of Biphenotypic Differentiation to Neutrophils or Macrophages, Independent of Enforced Meis Expression
Mol. Cell. Biol.,
May 1, 2000;
20(9):
3274 - 3285.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. Verbeek, J. Lekstrom-Himes, D. J. Park, P. M.-C. Dang, P. T. Vuong, S. Kawano, B. M. Babior, K. Xanthopoulos, and H. P. Koeffler
Myeloid Transcription Factor C/EBPvarepsilon Is Involved in the Positive Regulation of Lactoferrin Gene Expression in Neutrophils
Blood,
November 1, 1999;
94(9):
3141 - 3150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Catt, S. Hawkins, A. Roman, W. Luo, and D. G. Skalnik
Overexpression of CCAAT Displacement Protein Represses the Promiscuously Active Proximal gp91phox Promoter
Blood,
November 1, 1999;
94(9):
3151 - 3160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. M. Jacobsen and D. G. Skalnik
YY1 Binds Five cis-Elements and Trans-activates the Myeloid Cell-restricted gp91phox Promoter
J. Biol. Chem.,
October 15, 1999;
274(42):
29984 - 29993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E.K. Moses, K.A. Freed, J.R. Higgins, and S.P. Brennecke
Alternative forms of a novel aspartyl protease gene are differentially expressed in human gestational tissues
Mol. Hum. Reprod.,
October 1, 1999;
5(10):
983 - 989.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Anderson, K. A. Smith, H. Perkin, G. Hermanson, C.-G. Anderson, D. J. Jolly, R. A. Maki, and B. E. Torbett
PU.1 and the Granulocyte- and Macrophage Colony-Stimulating Factor Receptors Play Distinct Roles in Late-Stage Myeloid Cell Differentiation
Blood,
October 1, 1999;
94(7):
2310 - 2318.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Balbin, A. Fueyo, V. Knauper, A. M. Pendas, J. M. Lopez, M. G. Jimenez, G. Murphy, and C. Lopez-Otin
Collagenase 2 (MMP-8) Expression in Murine Tissue-remodeling Processes. ANALYSIS OF ITS POTENTIAL ROLE IN POSTPARTUM INVOLUTION OF THE UTERUS
J. Biol. Chem.,
September 11, 1998;
273(37):
23959 - 23968.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. D. Lawson and N. Berliner
Representational difference analysis of a committed myeloid progenitor cell line reveals evidence for bilineage potential
PNAS,
August 18, 1998;
95(17):
10129 - 10133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Hawkins, T. Kohwi-Shigematsu, and D. G. Skalnik
The Matrix Attachment Region-binding Protein SATB1 Interacts with Multiple Elements within the gp91phox Promoter and Is Down-regulated during Myeloid Differentiation
J. Biol. Chem.,
November 21, 2001;
276(48):
44472 - 44480.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
