|
|
 Previous Article | Table of Contents | Next Article 
Apoptotic Signal of Fas Is Not Mediated by Ceramide
Shu-Ching Hsu,
Chia-Cheng Wu,
Tien-Yau Luh,
Chen-Kung Chou,
Shau-Hwa Han, and
Ming-Zong Lai
From the Graduate Institute of Microbiology, National Taiwan
University School of Medicine, Taiwan, China; the Institute of
Molecular Biology, Academia Sinica, Taiwan, China; the Graduate
Institute of Microbiology and Immunology, National Yang-Ming
University, Taiwan, China; the Department of Chemistry, National Taiwan
University, Taiwan, China; and the Department of Medical Research,
Veteran General Hospital, Taipei, Taiwan, China.
Ceramide has been suggested as the secondary messenger mediating the
apoptotic signal for Fas engagement. By using different inhibitors, we
demonstrated here that ceramide is unlikely a mediator of Fas-initiated
apoptosis. First, cAMP prevented cell death induced by ceramide but not
by Fas. Second, ceramide-triggered, but not Fas-triggered, apoptosis
was antagonized by the free radical scavenger C60. Third,
the metal chelator pyrrolidinedithiocarbamate suppressed ceramide-initiated DNA fragmentation but had no effect on the Fas-induced cell death. Fourth, the SAPK/ERK kinase dominant negative mutant, which attenuated ceramide-induced cell death, did not prevent
Fas-induced apoptosis. Finally, activation of NF- B inhibited ceramide-induced but not Fas-initiated apoptosis. The fact that many
antagonists of ceramide-induced apoptosis could not suppress Fas-mediated cell death clearly indicates that ceramide is not the
mediator for Fas-initiated apoptotic signal.
Blood, Vol. 91 No. 8 (April 15), 1998:
pp. 2658-2663
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J.-J. Yin, F. Lao, J. Meng, P. P. Fu, Y. Zhao, G. Xing, X. Gao, B. Sun, P. C. Wang, C. Chen, et al.
Inhibition of Tumor Growth by Endohedral Metallofullerenol Nanoparticles Optimized as Reactive Oxygen Species Scavenger
Mol. Pharmacol.,
October 1, 2008;
74(4):
1132 - 1140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. S. Soderstrom, S. D. Nyberg, and J. E. Eriksson
CD95 capping is ROCK-dependent and dispensable for apoptosis
J. Cell Sci.,
May 15, 2005;
118(10):
2211 - 2223.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Tsao, T.-Y. Luh, C.-K. Chou, T.-Y. Chang, J.-J. Wu, C.-C. Liu, and H.-Y. Lei
In vitro action of carboxyfullerene
J. Antimicrob. Chemother.,
April 1, 2002;
49(4):
641 - 649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Tsao, T.-Y. Luh, C.-K. Chou, J.-J. Wu, Y.-S. Lin, and H.-Y. Lei
Inhibition of Group A Streptococcus Infection by Carboxyfullerene
Antimicrob. Agents Chemother.,
June 1, 2001;
45(6):
1788 - 1793.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Sun, R. Taniguchi, D. Tanoue, T. Yamaji, H. Takematsu, K. Mori, T. Fujita, T. Kawasaki, and Y. Kozutsumi
Sli2 (Ypk1), a Homologue of Mammalian Protein Kinase SGK, Is a Downstream Kinase in the Sphingolipid-Mediated Signaling Pathway of Yeast
Mol. Cell. Biol.,
June 15, 2000;
20(12):
4411 - 4419.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
U. Ramenghi, S. Bonissoni, G. Migliaretti, S. DeFranco, F. Bottarel, C. Gambaruto, D. DiFranco, R. Priori, F. Conti, I. Dianzani, et al.
Deficiency of the Fas apoptosis pathway without Fas gene mutations is a familial trait predisposing to development of autoimmune diseases and cancer
Blood,
May 15, 2000;
95(10):
3176 - 3182.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Ashush, L. A. Rozenszajn, M. Blass, M. Barda-Saad, D. Azimov, J. Radnay, D. Zipori, and U. Rosenschein
Apoptosis Induction of Human Myeloid Leukemic Cells by Ultrasound Exposure
Cancer Res.,
February 1, 2000;
60(4):
1014 - 1020.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Mori, Y. Terui, M. Ikeda, H. Tomizuka, M. Uwai, T. Kasahara, N. Kubota, T. Itoh, Y. Mishima, M. Douzono-Tanaka, et al.
beta 2-Microglobulin Identified as an Apoptosis-Inducing Factor and Its Characterization
Blood,
October 15, 1999;
94(8):
2744 - 2753.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-C. Hsu, M. A. Gavrilin, M.-H. Tsai, J. Han, and M.-Z. Lai
p38 Mitogen-activated Protein Kinase Is Involved in Fas Ligand Expression
J. Biol. Chem.,
September 3, 1999;
274(36):
25769 - 25776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Tsao, P. P. Kanakamma, T.-Y. Luh, C.-K. Chou, and H.-Y. Lei
Inhibition of Escherichia coli-Induced Meningitis by Carboxyfullerence
Antimicrob. Agents Chemother.,
September 1, 1999;
43(9):
2273 - 2277.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Hong, M.-D. Lai, Y.-S. Lin, and M.-Z. Lai
Antagonism of p53-dependent Apoptosis by Mitogen Signals
Cancer Res.,
June 1, 1999;
59(12):
2847 - 2852.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-H. Yeh, S.-C. Hsu, S.-H. Han, and M.-Z. Lai
Mitogen-activated Protein Kinase Kinase Antagonized Fas-associated Death Domain Protein-mediated Apoptosis by Induced FLICE-inhibitory Protein Expression
J. Exp. Med.,
November 16, 1998;
188(10):
1795 - 1802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Cuvillier, L. Edsall, and S. Spiegel
Involvement of Sphingosine in Mitochondria-dependent Fas-induced Apoptosis of Type II Jurkat T Cells
J. Biol. Chem.,
May 19, 2000;
275(21):
15691 - 15700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-C. Chen, T.-L. Hsu, T.-Y. Luh, and S.-L. Hsieh
Overexpression of Bcl-2 Enhances LIGHT- and Interferon-gamma -mediated Apoptosis in Hep3BT2 Cells
J. Biol. Chem.,
December 1, 2000;
275(49):
38794 - 38801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
