|
|
 Previous Article | Table of Contents | Next Article 
Cytokine Production and Function in c-mpl-Deficient Mice: No
Physiologic Role for Interleukin-3 in Residual Megakaryocyte and
Platelet Production
Timothy Gainsford,
Andrew W. Roberts,
Shinya Kimura,
Donald Metcalf,
Glenn Dranoff,
Richard C. Mulligan,
C. Glenn Begley,
Lorraine Robb, and
Warren S. Alexander
From The Walter and Eliza Hall Institute for Medical Research, PO
Royal Melbourne Hospital, Victoria, Australia; the Dana Farber Cancer
Institute, Boston; and the Howard Hughes Medical Institute, Harvard
University School of Medicine, Boston, MA.
Mice lacking thrombopoietin (TPO), or its receptor c-Mpl, display
defective megakaryocyte and platelet development and deficiencies in
progenitor cells of multiple hematopoietic lineages. The contribution of alternative cytokines to thrombopoiesis in the absence of TPO signalling was examined in mpl / mice.
Analysis of serum and organ-conditioned media showed no evidence of a
compensatory overproduction of megakaryocytopoietic cytokines. However,
consistent with a potential role in vivo, when injected into
mpl/ mice, interleukin-6 (IL-6) and
leukemia inhibitory factor (LIF) retained the capacity to elevate
megakaryocytes and their progenitors in hematopoietic tissues and
increase circulating platelet numbers. However, double mutant mice bred
to carry genetic defects both in c-Mpl and IL-3 or the alpha chain of
the IL-3 receptor, displayed no greater deficiencies in megakaryocytes
or platelets than mpl-deficient animals, suggesting absence of
a physiologic role for IL-3 in the residual megakaryocytopoiesis and
platelet production in these mice.
Blood, Vol. 91 No. 8 (April 15), 1998:
pp. 2745-2752
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Antonchuk, C. D. Hyland, D. J. Hilton, and W. S. Alexander
Synergistic effects on erythropoiesis, thrombopoiesis, and stem cell competitiveness in mice deficient in thrombopoietin and steel factor receptors
Blood,
September 1, 2004;
104(5):
1306 - 1313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Sitnicka, C. Brakebusch, I.-L. Martensson, M. Svensson, W. W. Agace, M. Sigvardsson, N. Buza-Vidas, D. Bryder, C. M.Cilio, H. Ahlenius, et al.
Complementary Signaling through flt3 and Interleukin-7 Receptor {alpha} Is Indispensable for Fetal and Adult B Cell Genesis
J. Exp. Med.,
November 17, 2003;
198(10):
1495 - 1506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Kuter and C. G. Begley
Recombinant human thrombopoietin: basic biology and evaluation of clinical studies
Blood,
November 15, 2002;
100(10):
3457 - 3469.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. C. Haznedaroglu, H. Goker, M. Turgut, Y. Buyukasik, and M. Benekli
Thrombopoietin as a Drug: Biologic Expectations, Clinical Realities, and Future Directions
Clinical and Applied Thrombosis/Hemostasis,
July 1, 2002;
8(3):
193 - 212.
[Abstract]
[PDF]
|
|
|
|
|
|
|
K. Kaushansky, N. Fox, N. L. Lin, and W. C. Liles
Lineage-specific growth factors can compensate for stem and progenitor cell deficiencies at the postprogenitor cell level: an analysis of doubly TPO- and G-CSF receptor-deficient mice
Blood,
May 15, 2002;
99(10):
3573 - 3578.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-H. Kie, W.-I. Yang, M.-K. Lee, T.-J. Kwon, Y.-H. Min, H.-O. Kim, H.-S. Ahn, S.-A. Im, H.-L. Kim, H.-Y. Park, et al.
Decrease in Apoptosis and Increase in Polyploidization of Megakaryocytes by Stem Cell Factor During Ex Vivo Expansion of Human Cord Blood CD34+ Cells Using Thrombopoietin
Stem Cells,
January 1, 2002;
20(1):
73 - 79.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Angchaisuksiri, S. R. Grigus, P. L. Carlson, G. W. Krystal, and E. N. Dessypris
Secretion of a unique peptide from interleukin-2-stimulated natural killer cells that induces endomitosis in immature human megakaryocytes
Blood,
January 1, 2002;
99(1):
130 - 136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Levin, L. Cocault, C. Demerens, C. Challier, M. Pauchard, J. Caen, and M. Souyri
Thrombocytopenic c-mpl{-}/{-} mice can produce a normal level of platelets after administration of 5-fluorouracil: the effect of age on the response
Blood,
August 15, 2001;
98(4):
1019 - 1027.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gillessen, N. Mach, C. Small, M. Mihm, and G. Dranoff
Overlapping roles for granulocyte-macrophage colony-stimulating factor and interleukin-3 in eosinophil homeostasis and contact hypersensitivity
Blood,
February 15, 2001;
97(4):
922 - 928.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Auernhammer and S. Melmed
Leukemia-Inhibitory Factor--Neuroimmune Modulator of Endocrine Function
Endocr. Rev.,
June 1, 2000;
21(3):
313 - 345.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Gainsford, H. Nandurkar, D. Metcalf, L. Robb, C. G. Begley, and W. S. Alexander
The residual megakaryocyte and platelet production in c-Mpl-deficient mice is not dependent on the actions of interleukin-6, interleukin-11, or leukemia inhibitory factor
Blood,
January 15, 2000;
95(2):
528 - 534.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. van der Loo and J. F. Martin
A Role for Changes in Platelet Production in the Cause of Acute Coronary Syndromes
Arterioscler. Thromb. Vasc. Biol.,
March 1, 1999;
19(3):
672 - 679.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
