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CD148 Is a Membrane Protein Tyrosine Phosphatase Present in All
Hematopoietic Lineages and Is Involved in Signal Transduction on
Lymphocytes
Miguel Angel de la Fuente-García,
Josep Maria Nicolás,
John H. Freed,
Eduard Palou,
Andrew P. Thomas,
Ramón Vilella,
Jordi Vives, and
Antoni Gayá
From the Servei d'Immunologia, Servei de Medicina Interna, Hospital
Clínic, Barcelona, Spain; the National Jewish Center for
Immunology and Respiratory Medicine, Denver, CO; and the Department of
Pathology, Anatomy and Cell Biology, Thomas Jefferson University,
Philadelphia, PA.
Evidence is presented showing that a protein tyrosine phosphatase
different from CD45 is present on the membrane of human hematopoietic
cells. The molecule recognized by the monoclonal antibody 143-41, which
has been classified as CD148 in the VI International Workshop on
Leukocyte Differentiation Antigens, was immunopurified and sequenced.
The sequence obtained from N-terminus as well as from two different
CNBr-digested peptides showed a close identity with a previously
described tyrosine phosphatase named HPTP- /DEP-1. CD148 is present
on all hematopoietic lineages, being expressed with higher intensity on
granulocytes than on monocytes and lymphocytes. Interestingly, whereas
it is clearly present on peripheral blood lymphocytes, it is poorly
expressed on different lymphoid cell lines of T and B origin. When this protein tyrosine phosphatase was cocrosslinked with CD3, an inhibition of the normally observed calcium mobilization was observed. This inhibition correlates with a decrease in phospholipase C- (PLC-) phosphorylation and is similar to the one observed with CD45. In
addition, it is shown that the crosslinking of the CD148 alone is also
able to induce an increase in [Ca2+]i. This
increase is abolished in the presence of genistein and by
cocrosslinking with CD45. These data, together with the induction of
tyrosine phosphorylation on several substrates, including PLC-, after CD148 crosslinking, suggest the involvement of a tyrosine kinase-based signaling pathway in this process. In conclusion, the data
presented show that CD148 corresponds to a previously described protein
tyrosine phosphatase HPTP-/DEP-1 and that this molecule is involved
in signal transduction in lymphocytes.
Blood, Vol. 91 No. 8 (April 15), 1998:
pp. 2800-2809
© 1998 by The American Society of Hematology.
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