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Interleukin-15 Is an Autocrine/Paracrine Viability Factor for
Cutaneous T-Cell Lymphoma Cells
Udo Döbbeling,
Reinhard Dummer,
Elisabeth Laine,
Natascha Potoczna,
Jian-Zhong Qin, and
Günter Burg
From the Department of Dermatology, University Hospital Zurich,
Switzerland.
In this study we investigated the role of interleukin-15 (IL-15) in
the immunobiology of cutaneous T-cell lymphoma (CTCL) cells. Using cell
culture techniques, reverse transcriptase-polymerase chain reaction
(RT-PCR), and immunhistochemistry we found that IL-15, like IL-7, is a
growth factor for the Sézary cell line SeAx and that both
cytokines prolonged the survival of malignant T cells directly isolated
from Sézary syndrome (SS) patients. Both IL-15 and IL-7 were more
potent than IL-2. IL-4 and IL-9, whose receptors share the same gamma
chain with the receptors of IL-2, IL-7, and IL-15, did not sustain the
growth of CTCL cells, indicating that signaling through the common
gamma chain ( c) is not sufficient for continuous growth. IL-13 and
tumor necrosis factor- (TNF-) had no effect. IL-7 and IL-15 also
supported the growth of SeAx cells in the presence of the apoptosis
inducing agents dexamethasone and retinoic acid. The analysis of
patient Sézary cells and three CTCL cell lines by RT-PCR showed
that all these cells expressed IL-15 mRNA, but only a few (25%)
produced IL-7 mRNA. Immunohistological analyses of skin biopsy samples of SS and Mycosis fungoides patients showed immunoreactivity for IL-15
in basal cell layer keratinocytes and in the infiltrating lymphocytes.
We conclude that IL-15 is a growth or viability factor for CTCL-derived
cell lines or shortly cultivated Sézary cells. The findings that
IL-15 mRNA can be detected in Sézary syndrome peripheral blood
mononuclear cells and that the IL-15 protein is detected in skin
sections from CTCL patients suggest that IL-15 plays an important role
in the biology of CTCL.
Blood, Vol. 92 No. 1 (July 1), 1998:
pp. 252-258
© 1998 by The American Society of Hematology.
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