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Interleukin-7 Influences the Development of Thymic Dendritic Cells
Alberto Varas,
Angeles Vicente,
Rosa Sacedón, and
Agustín G. Zapata
From the Department of Cell Biology, Faculty of Biology, Complutense
University of Madrid, Madrid, Spain.
Interleukin-7 (IL-7) has been shown to be a critical factor in B and
T lymphopoiesis, and to influence the differentiation of myeloid cell
lineages. In the present study we extend these results demonstrating
that IL-7 also plays an important role in the development of thymic
dendritic cells (DC). The addition of IL-7 to rat fetal thymus organ
cultures (FTOC) resulted in a drastic increase in the number of
CD3 CD4CD8 cells, which
mostly expressed typical DC markers, including major histocompatibility
complex class II, OX-62, CD11b, CD68, and CD54. These cells exhibited
morphological and ultrastructural features of DC, and were potent
stimulators of the allogeneic mixed leukocyte reaction. Although
increased numbers of DC were continuously generated throughout the
culture period in the presence of IL-7, they were not actively
dividing, indicating that DC in IL-7-treated cultures did not arise by
expansion of pre-existing cells. Reduced DC numbers obtained after the
addition of neutralizing anti-IL-7 antibodies to mouse FTOC confirmed
the relevance of endogenously produced IL-7 on thymic DC development.
Furthermore, the addition of IL-7 to FTOC derived from severe combined
immunodeficient mice also generated large numbers of DC in the absence
of thymocyte maturation.
Blood, Vol. 92 No. 1 (July 1), 1998:
pp. 93-100
© 1998 by The American Society of Hematology.
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