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Blood, Vol. 92 No. 11 (December 1), 1998:
pp. 4446-4452
P-Selectin and Platelet Clearance
Gaëtan Berger,
Daqing W. Hartwell, and
Denisa D. Wagner
From The Center for Blood Research, Harvard Medical School, Boston,
MA.
P-selectin is an adhesion receptor for leukocytes expressed by
activated platelets and endothelial cells. To assess a possible role of
P-selectin in platelet clearance, we adapted an in vivo biotinylation
technique in mice. Wild-type and P-selectin-deficient mice were
infused with N-hydroxysuccinimido biotin. The survival of biotinylated
platelets was followed by flow cytometry after labeling with
fluorescent streptavidin. Both wild-type and P-selectin-deficient platelets presented identical life spans of about 4.7 days, suggesting that P-selectin does not play a role in platelet turnover. When biotinylated platelets were isolated, activated with thrombin, and
reinjected into mice, the rate of platelet clearance was unchanged. In
contrast, storage of platelets at 4°C caused a significant reduction in their life span in vivo but again no significant differences were observed between the two genotypes. The infused thrombin-activated platelets rapidly lost their surface P-selectin in
circulation, and this loss was accompanied by the simultaneous appearance of a 100-kD P-selectin fragment in the plasma. This observation suggests that the platelet membrane P-selectin was shed by
cleavage. In conclusion, this study shows that P-selectin, despite its
binding to leukocytes, does not mediate platelet clearance. However,
the generation of a soluble form of P-selectin on platelet activation
may have biological implications in modulating leukocyte recruitment or
thrombus growth.
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