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Blood, Vol. 92 No. 3 (August 1), 1998:
pp. 756-764
RAPID COMMUNICATION
The  -Chemokine Receptor CXCR4 Is Expressed on the
Megakaryocytic Lineage From Progenitor to Platelets and Modulates
Migration and Adhesion
Jian-Feng Wang,
Zhong-Ying Liu, and
Jerome E. Groopman
From the Divisions of Experimental Medicine and Hematology/Oncology,
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston,
MA.
CXCR4 is the receptor for the  -chemokine stromal cell-derived
factor 1 (SDF-1) and has been shown to be expressed on a diversity of
leukocytes. In this report, the expression of the CXCR4 receptor in
cells of megakaryocytic lineage and the role of SDF-1 in
megakaryocytopoiesis were investigated. Using flow cytometry in
combination with reverse transcriptase-polymerase chain reaction
(RT-PCR), we observed that bone marrow CD34+,
CD61+ cells, blood platelets, and megakaryocytic leukemia
cell lines all expressed the CXCR4 receptor. To examine the expression
of the CXCR4 receptor on megakaryocyte progenitors (colony-forming units-megakaryocyte [CFU-Meg]), CXCR4-positive and
-negative CD34+ populations were separated from bone
marrow and cultured in a plasma clot culture system. A subpopulation of
the CFU-Meg was found in the CXCR4-positive fraction. The functional
significance of CXCR4 expression on cells of the megakaryocytic lineage
was examined by studying the effects of SDF-1 on migration and
proliferation of megakaryocyte progenitor cells in vitro. We found that
SDF-1 potently induced megakaryocyte progenitor migration and
significantly enhanced adhesion of mature marrow megakaryocytes
to endothelium. No marked effects of SDF-1 alone or in combination
with thrombopoietin and stem cell factor/kit ligand on megakaryocyte
production in vitro were noted. These results demonstrate for the first
time that the CXCR4 -chemokine receptor is expressed on cells of the megakaryocytic lineage from progenitors to platelets and that its
ligand SDF-1 may modulate several aspects of megakaryocytopoiesis.
© 1998 by The American Society of Hematology.
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