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Blood, Vol. 92 No. 3 (August 1), 1998:
pp. 842-848
Different Adhesive Characteristics and VLA-4 Expression of
CD34+ Progenitors in G0/G1 Versus
S+G2/M Phases of the Cell Cycle
Miki Yamaguchi,
Kenji Ikebuchi,
Fumiya Hirayama,
Norihiro Sato,
Yuko Mogi,
Jun-ichi Ohkawara,
Yoshihiro Yoshikawa,
Ken-ichi Sawada,
Takao Koike, and
Sadayoshi Sekiguchi
From Hokkaido Red Cross Blood Center, Sapporo; and the Department of
Internal Medicine II, Hokkaido University School of Medicine, Sapporo,
Japan.
We identified the cell cycle status of CD34+ cells of
steady-state bone marrow (BM) and peripheral blood (PB) obtained from healthy volunteers, and those of apherasis PB samples collected from
healthy donors who had been administered granulocyte colony-stimulating factor (G-CSF). More than 10% of CD34+ cells in BM were
in S+G2/M phase. In contrast, regardless of whether G-CSF
treatment was performed, less than 2% of CD34+ cells in
PB were cycling. BM CD34+ cells showed greater VLA-4
expression and adherence to stromal cells than PB CD34+
cells. In addition, when cycling and dormant BM CD34+
cells were analyzed separately, the cells in S+G2/M phase
expressed more VLA-4 and adhered to the stromal cell monolayer more
efficiently than the cells in G0/G1 phase.
Furthermore, this adhesion of CD34+ cells to the stromal
cell layer was almost completely inhibited by anti-VLA-4
antibody. Taken together, these results suggest that
CD34+ progenitors in G0/G1 phase
of the cell cycle differ from those in S+G2/M phase in
adhesiveness mediated by VLA-4 in the hematopoietic microenvironment.
© 1998 by The American Society of Hematology.
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