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Blood, Vol. 92 No. 5 (September 1), 1998:
pp. 1598-1607
Proliferation in Monocyte-Derived Dendritic Cell Cultures Is
Caused by Progenitor Cells Capable of Myeloid Differentiation
Lois L. Cavanagh,
Russell J. Saal,
Karen L. Grimmett, and
Ranjeny Thomas
From the Centre for Immunology and Cancer Research, the Department of
Medicine, University of Queensland; and the Department of Haematology,
Princess Alexandra Hospital, Queensland, Australia.
Dendritic cells (DC) can be generated by culture of adherent
peripheral blood (PB) cells in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). There is
controversy as to whether these DC arise from proliferating precursors
or simply from differentiation of monocytes. DC were generated from
myeloid-enriched PB non-T cells or sorted monocytes. DC generated from
either population functioned as potent antigen-presenting cells. Uptake
of [3H]-thymidine was observed in DC cultured from
myeloid-enriched non-T cells. Addition of lipopolysaccharide or tumor
necrosis factor- led to maturation of the DC, but did not inhibit
proliferation. Ki67+ cells were observed in cytospins of
these DC, and by double staining were
CD3 CD19CD11cCD40
and myeloperoxidase+, suggesting that they were myeloid
progenitor cells. Analysis of the starting population by flow cytometry
demonstrated small numbers of
CD34+CD33CD14 progenitor
cells, and numerous granulocyte-macrophage colony-forming units were
generated in standard assays. Thus, production of DC in vitro from
adherent PB cells also enriches for progenitor cells that are capable
of proliferation after exposure to GM-CSF. Of clinical importance, the
yield of DC derived in the presence of GM-CSF and IL-4 cannot be
expanded beyond the number of starting monocytes.
© 1998 by The American Society of Hematology.
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