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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Brown, E. T. Articles by Fuller, G. M. Search for Related Content PubMed PubMed Citation Articles by Brown, E. T. Articles by Fuller, G. M. Related Collections Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 92 No. 9 (November 1), 1998: pp. 3286-3293 Detection of a Complex That Associates With the B Fibrinogen G455-A Polymorphism Erika T. Brown and Gerald M. Fuller From the Laboratory of Medical Genetics and Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL. The promoter region of the B fibrinogen gene containing the polymorphic site (G455-A) shows an increase in fibrinogen levels for individuals containing an adenine rather than a guanine. Two methods were used to explore the possible functional role of this region. Electrophoretic mobility shift assays (EMSAs) were performed using specific DNA probes containing base sequences pertinent to the allelic site. Specific DNA binding proteins were detected and their binding characteristics were determined. Secondly, we placed DNA fragments containing different 455 nucleotide substitutions of the B promoter upstream of a luciferase reporter gene and transfected them into HepG2 cells to determine their effect on transactivation. An adenine at position 455 resulted in greater luciferase activity than when a guanine was present. UV cross-linking bound protein to the DNA demonstrated a 47-kD protein binding preferentially to the site when a guanine rather than an adenine was present at 455. We hypothesize that a transactivation protein complex associates with the site, but its association is stronger when guanine is present, thereby slowing downstream B gene transcription. These data provide the first molecular evidence that accounts for the increase in fibrinogen in individuals carrying this allele. © 1998 by The American Society of Hematology. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: W. Koch, P. Hoppmann, J. Biele, J. C. Mueller, A. Schomig, and A. Kastrati Fibrinogen Genes and Myocardial Infarction: A Haplotype Analysis Arterioscler Thromb Vasc Biol, April 1, 2008; 28(4): 758 - 763. [Abstract] [Full Text] [PDF] S. Kathiresan, Q. Yang, M. G. Larson, A. L. Camargo, G. H. Tofler, J. N. Hirschhorn, S. B. Gabriel, and C. J. O'Donnell Common Genetic Variation in Five Thrombosis Genes and Relations to Plasma Hemostatic Protein Level and Cardiovascular Disease Risk Arterioscler Thromb Vasc Biol, June 1, 2006; 26(6): 1405 - 1412. [Abstract] [Full Text] [PDF] M. Verschuur, M. de Jong, L. Felida, M. P. M. de Maat, and H. L. Vos A Hepatocyte Nuclear Factor-3 Site in the Fibrinogen {beta} Promoter Is Important for Interleukin 6-induced Expression, and Its Activity Is Influenced by the Adjacent -148C/T Polymorphism J. Biol. Chem., April 29, 2005; 280(17): 16763 - 16771. [Abstract] [Full Text] [PDF] H. Xia Regulation of {gamma}-Fibrinogen Chain Expression by Heterogeneous Nuclear Ribonucleoprotein A1 J. Biol. Chem., April 1, 2005; 280(13): 13171 - 13178. [Abstract] [Full Text] [PDF] E. M. Scott, R. A.S. Ariens, and P. J. Grant Genetic and Environmental Determinants of Fibrin Structure and Function: Relevance to Clinical Disease Arterioscler Thromb Vasc Biol, September 1, 2004; 24(9): 1558 - 1566. [Abstract] [Full Text] [PDF] B. Voetsch and J. Loscalzo Genetic Determinants of Arterial Thrombosis Arterioscler Thromb Vasc Biol, February 1, 2004; 24(2): 216 - 229. [Abstract] [Full Text] M. Martiskainen, T. Pohjasvaara, J. Mikkelsson, R. Mantyla, T. Kunnas, P. Laippala, E. Ilveskoski, M. Kaste, P.J. Karhunen, and T. Erkinjuntti Fibrinogen Gene Promoter -455 A Allele as a Risk Factor for Lacunar Stroke Stroke, April 1, 2003; 34(4): 886 - 891. [Abstract] [Full Text] [PDF] L. Iacoviello, M. Vischetti, F. Zito, and M. Benedetta Donati Genes Encoding Fibrinogen and Cardiovascular Risk Hypertension, November 1, 2001; 38(5): 1199 - 1203. [Abstract] [Full Text] [PDF] F. M. van ’t Hooft, S. J. F. von Bahr, A. Silveira, A. Iliadou, P. Eriksson, and A. Hamsten Two Common, Functional Polymorphisms in the Promoter Region of the {beta}-Fibrinogen Gene Contribute to Regulation of Plasma Fibrinogen Concentration Arterioscler Thromb Vasc Biol, December 1, 1999; 19(12): 3063 - 3070. [Abstract] [Full Text] [PDF]

	Copyright © 1998 by American Society of Hematology         Online ISSN: 1528-0020