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Blood, Vol. 92 No. 9 (November 1), 1998:
pp. 3346-3354
Alloantigen-Stimulated Anti-HIV Activity
Ligia A. Pinto,
Sandra Sharpe,
David I. Cohen, and
Gene M. Shearer
From the Experimental Immunology Branch and Laboratory of Tumor Cell
Biology, National Cancer Institute, National Institutes of Health,
Bethesda, MD.
A number of studies have suggested that an immune response to human
leukocyte antigen (HLA) alloantigens may contribute to protection
against HIV infection. In the present study, we examined the effect of
alloantigen-stimulated cell lines obtained from peripheral blood
mononuclear cells (PBMC) of HIV-uninfected (HIV )
individuals and the soluble factors produced by these cell lines on
HIV-1 replication. Multiple in vitro restimulation with irradiated allogeneic PBMC from HIV donors resulted in the
expansion of CD8+ T-cell lines that inhibited HIV-1
replication when cocultured with either autologous or heterologous in
vitro-infected phytohemagglutinin (PHA) blasts. Supernatants from the
alloantigen-stimulated cell lines also inhibited HIV replication in
both PHA blasts and a chronically infected cell line. The
alloantigen-stimulated cell lines and the factors they produced
inhibited both T-cell-tropic (T) and macrophage-tropic (M) isolates of
HIV-1. Blocking experiments using anti-chemokine antibodies suggested
that this inhibition of HIV replication was not due to the
 -chemokines present in cocultures of cell lines with
HIV-infected blasts. These results indicate that
alloantigen-stimulation of PBMC from HIV
individuals activates CD8+ T cells that produce
soluble factor(s) that inhibit HIV replication of a wide spectrum of
HIV-1 isolates through a chemokine-independent mechanism.
This is a US government work. There are no restrictions on its use.
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