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Blood, Vol. 93 No. 1 (January 1), 1999:
pp. 251-259
Hairy Cell Leukemia-Specific Recognition by Multiple Autologous
HLA-DQ or DP-Restricted T-Cell Clones
Lisette van de Corput,
Hanneke C. Kluin-Nelemans,
Michel G.D. Kester,
Roel Willemze, and
J.H. Frederik Falkenburg
From the Laboratory of Experimental Hematology, Department of
Hematology, Leiden University Medical Center, Leiden, The Netherlands.
We studied in patients with hairy cell leukemia (HCL) whether
autoreactive T cells could be isolated with specific reactivity to the
HCL cells. HCL cells were activated via triggering of CD40 on the cell
membrane and used as stimulator cells to generate autologous T-cell
clones. Two types of CD4+BV2+ T-cell clones
with different CDR3 rearrangements and one type of
CD4+BV8S3+ T-cell clone were generated from
the spleen or blood. These clones specifically recognized the
autologous HCL cells, without reactivity to autologous peripheral blood
mononuclear cells (PBMC), phytohemagglutinin blasts, or Epstein-Barr
virus-transformed B cells in a primed lymphocyte test. Blocking and
panel studies using HCL cells from 11 other patients showed that
recognition of the HCL cells by the BV2+ T cells was
restricted by HLA-DQA1*03/DQB1*0301, and the BV8S3+ T
cells were restricted by DPB1*04. The T-cell clones did not recognize
DPB1*04+ or DQ3+ PBMC from healthy donors
or DP/DQ matched malignant cells from patients with other hematologic
malignancies, except for one patient with acute lymphoblastic leukemia.
These HCL-specific T-cell clones may be used for the detection of an
HCL-specific tumor antigen.
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