|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 10 (May 15), 1999:
pp. 3309-3316
Interferon  Induces Upregulation and Activation of Caspases 1, 3, and 8 to Produce Apoptosis in Human Erythroid Progenitor Cells
Chunhua Dai and
Sanford B. Krantz
From the Hematology/Oncology Division, Department of Medicine,
Department of Veterans Affairs Medical Center, Nashville, TN; and the
Vanderbilt Cancer Center, Vanderbilt University School of Medicine,
Nashville, TN.
Interferon  (IFN) induces apoptosis in purified human
erythroid colony-forming cells (ECFC) and inhibits cell growth. Fas (APO-1; CD95) and Fas ligand (FasL) mediate apoptosis induced by
IFN, because Fas is significantly upregulated by IFN, whereas Fas
ligand is constitutively present in the ECFC and neutralization of FasL
greatly reduces the apoptosis. Because conversion of caspases from
their dormant proenzyme forms to active enzymes has a critical role in
transducing a cascade leading to apoptosis, we performed further
studies of the expression and activation of caspases in normal human
and IFN-treated day-6 ECFC to better understand the mechanism of
IFN action in producing this cell death. RNase protection assays
showed that the caspase-1, -2, -6, -8, and -9 mRNAs were upregulated by
IFN, whereas the caspase-5 and -7 mRNAs were not increased. Western
blots showed that FLICE/caspase-8 was upregulated and activated by 24 hours of incubation with IFN. FADD was not similarly altered by
incubation with IFN. Western blots of ICE/caspase-1, which might be
required for amplification of the initial FLICE activation signal,
showed that pro-ICE expression significantly increased after treatment
with IFN for 24 hours and cleavage of pro-ICE also increased.
CPP32/apopain/caspase-3, responsible for the proteolytic cleavage of
poly (ADP) ribose polymerase (PARP), was also studied and treatment of
ECFC with IFN resulted in an increased concentration of caspase-3 by
24 hours and a clear induction of enzyme activation by 48 hours, which
was identified by the appearance of its p17-kD peptide fragment. The
cleavage of PARP was demonstrated by an obvious increase of the 89-kD
PARP cleavage product, which was observed at almost the same time as
caspase-3 activation in the IFN-treated cells, whereas untreated
ECFC showed little change. Peptide inhibitors of the caspase proteins,
DEVD-fmk, DEVD-cho, YVAD-cho, and IETD-fmk, were incubated with the
ECFC to obtain further evidence for the involvement of caspases in
IFN-induced apoptosis. The activation of FLICE/caspase-8 and
CPP32/caspase-3 and cleavage of PARP clearly were inhibited, but the
reduction of cell growth due to apoptosis, induced by IFN, was only
partially blocked by the presence of the inhibitors. These results
indicate that IFN acts on ECFC not only to upregulate Fas, but also
to selectively upregulate caspases-1, -3, and -8, which are activated
and produce apoptosis, whereas the concentrations of FasL and FADD are
not demonstrably changed.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. E. Randall and S. Goodbourn
Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures
J. Gen. Virol.,
January 1, 2008;
89(1):
1 - 47.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. N. Baden, J. Murray, R. A. Capaldi, and K. Guillemin
Early Developmental Pathology Due to Cytochrome c Oxidase Deficiency Is Revealed by a New Zebrafish Model
J. Biol. Chem.,
November 30, 2007;
282(48):
34839 - 34849.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Lissat, T. Vraetz, M. Tsokos, R. Klein, M. Braun, N. Koutelia, P. Fisch, M. E. Romero, L. Long, P. Noellke, et al.
Interferon-{gamma} Sensitizes Resistant Ewing's Sarcoma Cells to Tumor Necrosis Factor Apoptosis-Inducing Ligand-Induced Apoptosis by Up-Regulation of Caspase-8 Without Altering Chemosensitivity
Am. J. Pathol.,
June 1, 2007;
170(6):
1917 - 1930.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ryu, J. Holzschuh, S. Erhardt, A.-K. Ettl, and W. Driever
Depletion of minichromosome maintenance protein 5 in the zebrafish retina causes cell-cycle defect and apoptosis
PNAS,
December 20, 2005;
102(51):
18467 - 18472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-P. Herbeuval, J.-C. Grivel, A. Boasso, A. W. Hardy, C. Chougnet, M. J. Dolan, H. Yagita, J. D. Lifson, and G. M. Shearer
CD4+ T-cell death induced by infectious and noninfectious HIV-1: role of type 1 interferon-dependent, TRAIL/DR5-mediated apoptosis
Blood,
November 15, 2005;
106(10):
3524 - 3531.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Felli, F. Pedini, A. Zeuner, E. Petrucci, U. Testa, C. Conticello, M. Biffoni, A. Di Cataldo, J. A. Winkles, C. Peschle, et al.
Multiple Members of the TNF Superfamily Contribute to IFN-{gamma}-Mediated Inhibition of Erythropoiesis
J. Immunol.,
August 1, 2005;
175(3):
1464 - 1472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Jiang, D. Gross, S. Nogusa, P. Elbaum, and D. M. Murasko
Depletion of T Cells by Type I Interferon: Differences between Young and Aged Mice
J. Immunol.,
August 1, 2005;
175(3):
1820 - 1826.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Florido, J. E. Pearl, A. Solache, M. Borges, L. Haynes, A. M. Cooper, and R. Appelberg
Gamma Interferon-Induced T-Cell Loss in Virulent Mycobacterium avium Infection
Infect. Immun.,
June 1, 2005;
73(6):
3577 - 3586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Mitra-Kaushik, J. Harding, J. Hess, R. Schreiber, and L. Ratner
Enhanced tumorigenesis in HTLV-1 Tax-transgenic mice deficient in interferon-gamma
Blood,
November 15, 2004;
104(10):
3305 - 3311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Sabbagh, S. M. Kaech, M. Bourbonniere, M. Woo, L. Y. Cohen, E. K. Haddad, N. Labrecque, R. Ahmed, and R.-P. Sekaly
The Selective Increase in Caspase-3 Expression in Effector but Not Memory T Cells Allows Susceptibility to Apoptosis
J. Immunol.,
November 1, 2004;
173(9):
5425 - 5433.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. W. Carlile, D. H. Smith, and M. Wiedmann
Caspase-3 has a nonapoptotic function in erythroid maturation
Blood,
June 1, 2004;
103(11):
4310 - 4316.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sun, T. A. Rothermel, L. Shuman, J. A. Aligo, S. Xu, Y. Lin, R. A. Lamb, and B. He
Conserved Cysteine-Rich Domain of Paramyxovirus Simian Virus 5 V Protein Plays an Important Role in Blocking Apoptosis
J. Virol.,
May 15, 2004;
78(10):
5068 - 5078.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Burchert, D. Cai, L. C. Hofbauer, M. K. R. Samuelsson, E. P. Slater, J. Duyster, M. Ritter, A. Hochhaus, R. Muller, M. Eilers, et al.
Interferon consensus sequence binding protein (ICSBP; IRF-8) antagonizes BCR/ABL and down-regulates bcl-2
Blood,
May 1, 2004;
103(9):
3480 - 3489.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kalai, M. Lamkanfi, G. Denecker, M. Boogmans, S. Lippens, A. Meeus, W. Declercq, and P. Vandenabeele
Regulation of the expression and processing of caspase-12
J. Cell Biol.,
August 4, 2003;
162(3):
457 - 467.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Garcia-Domingo, D. Ramirez, G. Gonzalez de Buitrago, and C. Martinez-A
Death Inducer-Obliterator 1 Triggers Apoptosis after Nuclear Translocation and Caspase Upregulation
Mol. Cell. Biol.,
May 1, 2003;
23(9):
3216 - 3225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Lin, A. C. Bright, T. A. Rothermel, and B. He
Induction of Apoptosis by Paramyxovirus Simian Virus 5 Lacking a Small Hydrophobic Gene
J. Virol.,
March 15, 2003;
77(6):
3371 - 3383.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Yang, M. S. Merchant, M. E. Romero, M. Tsokos, L. H. Wexler, U. Kontny, C. L. Mackall, and C. J. Thiele
Induction of Caspase 8 by Interferon {gamma} Renders Some Neuroblastoma (NB) Cells Sensitive to Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL) but Reveals That a Lack of Membrane TR1/TR2 Also Contributes to TRAIL Resistance in NB
Cancer Res.,
March 1, 2003;
63(5):
1122 - 1129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I.-J. Chung, C. Dai, and S. B. Krantz
Stem cell factor increases the expression of FLIP that inhibits IFNgamma -induced apoptosis in human erythroid progenitor cells
Blood,
February 15, 2003;
101(4):
1324 - 1328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Cooper, C. P. Breen, B. Vyas, J. Ochola, D. M. Kemeny, and I. C. Macdougall
Poor response to recombinant erythropoietin is associated with loss of T-lymphocyte CD28 expression and altered interleukin-10 production
Nephrol. Dial. Transplant.,
January 1, 2003;
18(1):
133 - 140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. D. Joshi, D. V. Kalvakolanu, J. D. Hasday, R. J. Hebel, and A. S. Cross
IL-18 Levels and the Outcome of Innate Immune Response to Lipopolysaccharide: Importance of a Positive Feedback Loop with Caspase-1 in IL-18 Expression
J. Immunol.,
September 1, 2002;
169(5):
2536 - 2544.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Cottet, P. Dupraz, F. Hamburger, W. Dolci, M. Jaquet, and B. Thorens
cFLIP Protein Prevents Tumor Necrosis Factor-{alpha}-Mediated Induction of Caspase-8-Dependent Apoptosis in Insulin-Secreting {beta}Tc-Tet Cells
Diabetes,
June 1, 2002;
51(6):
1805 - 1814.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. E. Kling, H. K. Lorenzo, A. M. Trbovich, T. B. Kinane, P. K. Donahoe, and J. J. Schnitzer
Pre- and Postnatal Lung Development, Maturation, and Plasticity: MEK-1/2 inhibition reduces branching morphogenesis and causes mesenchymal cell apoptosis in fetal rat lungs
Am J Physiol Lung Cell Mol Physiol,
March 1, 2002;
282(3):
L370 - L378.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Silvestris, P. Cafforio, M. Tucci, and F. Dammacco
Negative regulation of erythroblast maturation by Fas-L+/TRAIL+ highly malignant plasma cells: a major pathogenetic mechanism of anemia in multiple myeloma
Blood,
February 15, 2002;
99(4):
1305 - 1313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Takeda, M. J. Smyth, E. Cretney, Y. Hayakawa, N. Kayagaki, H. Yagita, and K. Okumura
Critical Role for Tumor Necrosis Factor-related Apoptosis-inducing Ligand in Immune Surveillance Against Tumor Development
J. Exp. Med.,
January 14, 2002;
195(2):
161 - 169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Kumar-Sinha, S. Varambally, A. Sreekumar, and A. M. Chinnaiyan
Molecular Cross-talk between the TRAIL and Interferon Signaling Pathways
J. Biol. Chem.,
January 4, 2002;
277(1):
575 - 585.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. A. Martin and A. Panja
Cytokine regulation of human intestinal primary epithelial cell susceptibility to Fas-mediated apoptosis
Am J Physiol Gastrointest Liver Physiol,
January 1, 2002;
282(1):
G92 - G104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Morita-Fujimura, M. Fujimura, T. Yoshimoto, and P. H. Chan
Superoxide During Reperfusion Contributes to Caspase-8 Expression and Apoptosis After Transient Focal Stroke
Stroke,
October 1, 2001;
32(10):
2356 - 2361.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yoshikawa, Y. Nakajima, and K. Tasaka
IFN-{gamma} Induces the Apoptosis of WEHI 279 and Normal Pre-B Cell Lines by Expressing Direct Inhibitor of Apoptosis Protein Binding Protein with Low pI
J. Immunol.,
September 1, 2001;
167(5):
2487 - 2495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Matsushima, M. Nakashima, K. Oshima, Y. Abe, J. Nishimura, H. Nawata, T. Watanabe, and K. Muta
Receptor binding cancer antigen expressed on SiSo cells, a novel regulator of apoptosis of erythroid progenitor cells
Blood,
July 15, 2001;
98(2):
313 - 321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Moreau, M. Leite-de-Moraes, S. Ezine, J. P. Di Santo, M. Dy, and E. Schneider
Natural killer cell-dependent apoptosis of peripheral murine hematopoietic progenitor cells in response to Fas cross-linking: involvement of tumor necrosis factor-{alpha}
Blood,
May 15, 2001;
97(10):
3069 - 3074.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. He, G. Y. Lin, J. E. Durbin, R. K. Durbin, and R. A. Lamb
The SH Integral Membrane Protein of the Paramyxovirus Simian Virus 5 Is Required To Block Apoptosis in MDBK Cells
J. Virol.,
May 1, 2001;
75(9):
4068 - 4079.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Silvestris, M. Tucci, P. Cafforio, and F. Dammacco
Fas-L up-regulation by highly malignant myeloma plasma cells: role in the pathogenesis of anemia and disease progression
Blood,
March 1, 2001;
97(5):
1155 - 1164.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. A. Street, E. Cretney, and M. J. Smyth
Perforin and interferon-{gamma} activities independently control tumor initiation, growth, and metastasis
Blood,
January 1, 2001;
97(1):
192 - 197.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Rathbun, T. A. Christianson, G. R. Faulkner, G. Jones, W. Keeble, M. O'Dwyer, and G. C. Bagby
Interferon-gamma -induced apoptotic responses of Fanconi anemia group C hematopoietic progenitor cells involve caspase 8-dependent activation of caspase 3 family members
Blood,
December 15, 2000;
96(13):
4204 - 4211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ruiz-Ruiz, C. Muñoz-Pinedo, and A. López-Rivas
Interferon-{{gamma}} Treatment Elevates Caspase-8 Expression and Sensitizes Human Breast Tumor Cells to a Death Receptor-induced Mitochondria-operated Apoptotic Program
Cancer Res.,
October 1, 2000;
60(20):
5673 - 5680.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
I. Choi, K. Muta, A. Wickrema, S. B. Krantz, J. Nishimura, and H. Nawata
Interferon gamma delays apoptosis of mature erythroid progenitor cells in the absence of erythropoietin
Blood,
June 15, 2000;
95(12):
3742 - 3749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Allione, P. Bernabei, M. Bosticardo, S. Ariotti, G. Forni, and F. Novelli
Nitric Oxide Suppresses Human T Lymphocyte Proliferation Through IFN-{gamma}-Dependent and IFN-{gamma}-Independent Induction of Apoptosis
J. Immunol.,
October 15, 1999;
163(8):
4182 - 4191.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Schneider, G. Moreau, A. Arnould, F. Vasseur, N. Khodabaccus, M. Dy, and S. Ezine
Increased Fetal and Extramedullary Hematopoiesis in Fas-Deficient C57BL/6-lpr/lpr Mice
Blood,
October 15, 1999;
94(8):
2613 - 2621.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Varela, C. Munoz-Pinedo, C. Ruiz-Ruiz, G. Robledo, M. Pedroso, and A. Lopez-Rivas
Interferon-gamma Sensitizes Human Myeloid Leukemia Cells to Death Receptor-mediated Apoptosis by a Pleiotropic Mechanism
J. Biol. Chem.,
May 18, 2001;
276(21):
17779 - 17787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
