|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 11 (June 1), 1999:
pp. 3750-3756
The Myeloid-Lymphoid Initiating Cell (ML-IC) Assay Assesses the
Fate of Multipotent Human Progenitors In Vitro
M. Punzel,
S.D. Wissink,
J.S. Miller,
K.A. Moore,
I.R. Lemischka, and
C.M. Verfaillie
From the Department of Medicine, Division of Hematology and Stem Cell
Biology Program, University of Minnesota, Minneapolis, MN; and the
Department of Molecular Biology, Princeton University, Princeton,
NJ.
Hematopoietic stem cells (HSC) are cells with self-renewing
multilineage differentiation potential. Although engraftment in xenogeneic recipients can be used to measure human HSC, these assays do
not allow assessment of individual progenitors. We developed an in
vitro assay that allows the identification of a single human bone
marrow progenitor closely related to HSC, which we termed "Myeloid-Lymphoid Initiating Cell," or ML-IC, because it is
capable of generating multiple secondary progenitors that can
reinitiate long-term myeloid and lymphoid hematopoiesis in vitro. The
assay is done in contact with murine AFT024 fetal liver stromal cells and with Flt3-Ligand, stem cell factor, and interleukin-7. In this
assay, 0.2% to 1.7% of Lin
 /34+/DRdim cells could
generate 1 to 3 long-term culture initiating cells (LTC-IC) as well as
1 to 4 NK-IC after 4 to 6 weeks. In addition, this assay measures
contribution of net-progenitor conservation and net-progenitor
proliferation over time, providing insight in the fate of individual
LTC-IC and NK-IC. This assay will prove useful to enumerate the number
of very primitive human progenitors with multilineage differentiation
potential, as well as to evaluate future ex vivo culture conditions.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Beckmann, S. Scheitza, P. Wernet, J. C. Fischer, and B. Giebel
Asymmetric cell division within the human hematopoietic stem and progenitor cell compartment: identification of asymmetrically segregating proteins
Blood,
June 15, 2007;
109(12):
5494 - 5501.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Giebel, T. Zhang, J. Beckmann, J. Spanholtz, P. Wernet, A. D. Ho, and M. Punzel
Primitive human hematopoietic cells give rise to differentially specified daughter cells upon their initial cell division
Blood,
March 1, 2006;
107(5):
2146 - 2152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. W. Storms, P. D. Green, K. M. Safford, D. Niedzwiecki, C. R. Cogle, O. M. Colvin, N. J. Chao, H. E. Rice, and C. A. Smith
Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34
Blood,
July 1, 2005;
106(1):
95 - 102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wagner, A. Ansorge, U. Wirkner, V. Eckstein, C. Schwager, J. Blake, K. Miesala, J. Selig, R. Saffrich, W. Ansorge, et al.
Molecular evidence for stem cell function of the slow-dividing fraction among human hematopoietic progenitor cells by genome-wide analysis
Blood,
August 1, 2004;
104(3):
675 - 686.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Ho and M. Punzel
Hematopoietic stem cells: can old cells learn new tricks?
J. Leukoc. Biol.,
May 1, 2003;
73(5):
547 - 555.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cashman, B. Dykstra, I. Clark-Lewis, A. Eaves, and C. Eaves
Changes in the Proliferative Activity of Human Hematopoietic Stem Cells in NOD/SCID Mice and Enhancement of Their Transplantability after In Vivo Treatment with Cell Cycle Inhibitors
J. Exp. Med.,
November 4, 2002;
196(9):
1141 - 1150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Hackney, P. Charbord, B. P. Brunk, C. J. Stoeckert, I. R. Lemischka, and K. A. Moore
A molecular profile of a hematopoietic stem cell niche
PNAS,
October 1, 2002;
99(20):
13061 - 13066.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. O. Muench and A. Barcena
Broad Distribution of Colony-Forming Cells with Erythroid, Myeloid, Dendritic Cell, and NK Cell Potential Among CD34++ Fetal Liver Cells
J. Immunol.,
November 1, 2001;
167(9):
4902 - 4909.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Reyes, T. Lund, T. Lenvik, D. Aguiar, L. Koodie, and C. M. Verfaillie
Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells
Blood,
November 1, 2001;
98(9):
2615 - 2625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Miller and V. McCullar
Human natural killer cells with polyclonal lectin and immunoglobulinlike receptors develop from single hematopoietic stem cells with preferential expression of NKG2A and KIR2DL2/L3/S2
Blood,
August 1, 2001;
98(3):
705 - 713.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. D. Lewis, G. Almeida-Porada, J. Du, I. R. Lemischka, K. A. Moore, E. D. Zanjani, and C. M. Verfaillie
Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system
Blood,
June 1, 2001;
97(11):
3441 - 3449.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
