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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by van Baarle, D. Articles by van Oers, M. H.J. Search for Related Content PubMed PubMed Citation Articles by van Baarle, D. Articles by van Oers, M. H.J. Related Collections Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 93 No. 11 (June 1), 1999: pp. 3949-3955 Direct Epstein-Barr Virus (EBV) Typing on Peripheral Blood Mononuclear Cells: No Association Between EBV Type 2 Infection or Superinfection and the Development of Acquired Immunodeficiency Syndrome-Related Non-Hodgkin's Lymphoma Debbie van Baarle, Egbert Hovenkamp, Marie José Kersten, Michèl R. Klein, Frank Miedema, and Marinus H.J. van Oers From the Department of Hematology, Academical Medical Center, Amsterdam; the Department of Clinical Viro-Immunology, CLB, Sanquin Blood Supply Foundation, Laboratory for Experimental and Clinical Immunology, Academical Medical Center, University of Amsterdam, Amsterdam; the Department of Immunology and Medical Oncology, Netherlands Cancer Institute, Antoni van Leeuwenhoek Ziekenhuis, Amsterdam; and the Department of Human Retrovirology, Academical Medical Center, University of Amsterdam, Amsterdam, The Netherlands. In the literature, a correlation has been suggested between the occurrence of acquired immunodeficiency syndrome (AIDS)-related non-Hodgkin's lymphomas (NHL) and Epstein-Barr virus (EBV) type 2 infection. To further investigate a possible role for EBV type 2 infection in the development of AIDS-NHL, we developed a sensitive and type-specific nested polymerase chain reaction (PCR) assay and analyzed EBV types directly on peripheral blood mononuclear cells (PBMC) in three subgroups of human immunodeficiency virus (HIV)-1 infected individuals: 30 AIDS-NHL patients, 42 individuals progressing to AIDS without lymphoma (PROG), either developing opportunistic infections (AIDS-OI) or Kaposi's sarcoma (AIDS-KS), and 18 long-term asymptomatic individuals (LTA). Furthermore, EBV type analysis was performed on PBMC samples obtained from AIDS-NHL patients in the course of HIV-1 infection. The results showed that: (1) direct analysis of PBMC is superior to analysis of B-lymphoblastoid cell lines (B-LCL) grown from the same PBMC samples; (2) in HIV-1 infected individuals, there is a high prevalence of EBV type 2 infection (50% in LTA, 62% in progressors, and 53% in AIDS-NHL) and superinfection with both type 1 and 2 (24% in LTA, 40% in progressors, and 47% in AIDS-NHL); (3) EBV type 2 (super)infection is not associated with an increased risk for development of AIDS-NHL; (4) type 2 infection can be found early in HIV-1 infection, and neither type 2 infection nor superinfection correlates with a failing immune system. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. M. Brooks, D. S. G. Croom-Carter, A. M. Leese, R. J. Tierney, G. Habeshaw, and A. B. Rickinson Cytotoxic T-Lymphocyte Responses to a Polymorphic Epstein-Barr Virus Epitope Identify Healthy Carriers with Coresident Viral Strains J. Virol., February 15, 2000; 74(4): 1801 - 1809. [Abstract] [Full Text]

	Copyright © 1999 by American Society of Hematology         Online ISSN: 1528-0020