|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 12 (June 15), 1999:
pp. 4196-4207
The Mouse GATA-2 Gene is Expressed in the Para-Aortic
Splanchnopleura and Aorta-Gonads and Mesonephros Region
Naoko Minegishi,
Jun Ohta,
Hironori Yamagiwa,
Norio Suzuki,
Shimako Kawauchi,
Yinghui Zhou,
Satoru Takahashi,
Norio Hayashi,
James Douglas Engel, and
Masayuki Yamamoto
From the Center for Tsukuba Advanced Research Alliance and Institute
of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan;
Department of Biochemistry, Tohoku University School of Medicine, 2-1 Seiryocho, Aoba-ku, Sendai, Japan; and Department of Biochemistry,
Molecular Biology and Cell Biology, Northwestern University, Evanston
IL.
We previously reported that the mouse GATA-2 gene is
regulated by two alternative promoters (Minegishi et al, J
Biol Chem, 273:3625, 1998). Although the more proximal IG (general)
promoter is active in almost all GATA-2-expressing cells, the distal
IS (specific) promoter activity was selectively detected in
hematopoietic tissues but not in other mesodermal tissues. We report
here in vivo analysis of the GATA-2 locus and its regulatory
characteristics in hematopoietic tissues of transgenic mice. Transgenes
containing 6 or 7 kbp of sequence flanking the 5' end of the IS
first exon direct expression of  -galactosidase or green fluorescent
protein (GFP) reporter genes specifically to the para-aortic
splanchnopleura, aorta-gonads, and mesonephros (AGM) region, and in the
neural tissues. In situ hybridization analysis showed that reporter
gene expression specifically recapitulates the endogenous expression profile of GATA-2 in these tissues. The flk-1, CD34, c-kit, and CD45
antigens were identified in the GFP-positive cells from the AGM region
and fetal liver, indicating that GATA-2 is expressed in immature
hematopoietic cells. Deletion of 3.5 kbp from the 5' end of the
6.0 kbp IS promoter construct, including one of the DNase I
hypersensitive sites, completely abolished hematopoietic expression.
These experiments describe an early developmental GATA-2 hematopoietic
enhancer located between 6.0 and 2.5 kbp 5' to the IS exon.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Peeters, K. Ottersbach, K. Bollerot, C. Orelio, M. de Bruijn, M. Wijgerde, and E. Dzierzak
Ventral embryonic tissues and Hedgehog proteins induce early AGM hematopoietic stem cell development
Development,
August 1, 2009;
136(15):
2613 - 2621.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Nozawa, N. Suzuki, M. Kobayashi-Osaki, X. Pan, J. D. Engel, and M. Yamamoto
GATA2-dependent and region-specific regulation of Gata2 transcription in the mouse midbrain
Genes Cells,
May 1, 2009;
14(5):
569 - 582.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Ditadi, P. de Coppi, O. Picone, L. Gautreau, R. Smati, E. Six, D. Bonhomme, S. Ezine, R. Frydman, M. Cavazzana-Calvo, et al.
Human and murine amniotic fluid c-Kit+Lin- cells display hematopoietic activity
Blood,
April 23, 2009;
113(17):
3953 - 3960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ray, D. Dutta, M. A. K. Rumi, L. N. Kent, M. J. Soares, and S. Paul
Context-dependent Function of Regulatory Elements and a Switch in Chromatin Occupancy between GATA3 and GATA2 Regulate Gata2 Transcription during Trophoblast Differentiation
J. Biol. Chem.,
February 20, 2009;
284(8):
4978 - 4988.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. T. Nottingham, A. Jarratt, M. Burgess, C. L. Speck, J.-F. Cheng, S. Prabhakar, E. M. Rubin, P.-S. Li, J. Sloane-Stanley, J. Kong-a-San, et al.
Runx1-mediated hematopoietic stem-cell emergence is controlled by a Gata/Ets/SCL-regulated enhancer
Blood,
December 15, 2007;
110(13):
4188 - 4197.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Wozniak, M. E. Boyer, J. A. Grass, Y. Lee, and E. H. Bresnick
Context-dependent GATA Factor Function: COMBINATORIAL REQUIREMENTS FOR TRANSCRIPTIONAL CONTROL IN HEMATOPOIETIC AND ENDOTHELIAL CELLS
J. Biol. Chem.,
May 11, 2007;
282(19):
14665 - 14674.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Martowicz, J. A. Grass, and E. H. Bresnick
GATA-1-mediated Transcriptional Repression Yields Persistent Transcription Factor IIB-Chromatin Complexes
J. Biol. Chem.,
December 8, 2006;
281(49):
37345 - 37352.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Grass, H. Jing, S.-I. Kim, M. L. Martowicz, S. Pal, G. A. Blobel, and E. H. Bresnick
Distinct Functions of Dispersed GATA Factor Complexes at an Endogenous Gene Locus.
Mol. Cell. Biol.,
October 1, 2006;
26(19):
7056 - 7067.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Moriguchi, M. Hamada, N. Morito, T. Terunuma, K. Hasegawa, C. Zhang, T. Yokomizo, R. Esaki, E. Kuroda, K. Yoh, et al.
MafB Is Essential for Renal Development and F4/80 Expression in Macrophages
Mol. Cell. Biol.,
August 1, 2006;
26(15):
5715 - 5727.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kobayashi-Osaki, O. Ohneda, N. Suzuki, N. Minegishi, T. Yokomizo, S. Takahashi, K.-C. Lim, J. D. Engel, and M. Yamamoto
GATA Motifs Regulate Early Hematopoietic Lineage-Specific Expression of the Gata2 Gene
Mol. Cell. Biol.,
August 15, 2005;
25(16):
7005 - 7020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. P. Rodrigues, V. Janzen, R. Forkert, D. M. Dombkowski, A. S. Boyd, S. H. Orkin, T. Enver, P. Vyas, and D. T. Scadden
Haploinsufficiency of GATA-2 perturbs adult hematopoietic stem-cell homeostasis
Blood,
July 15, 2005;
106(2):
477 - 484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Minegishi, N. Suzuki, Y. Kawatani, R. Shimizu, and M. Yamamoto
Rapid turnover of GATA-2 via ubiquitin-proteasome protein degradation pathway
Genes Cells,
July 1, 2005;
10(7):
693 - 704.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Robert-Moreno, L. Espinosa, J. L. de la Pompa, and A. Bigas
RBPj{kappa}-dependent Notch function regulates Gata2 and is essential for the formation of intra-embryonic hematopoietic cells
Development,
March 1, 2005;
132(5):
1117 - 1126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Martowicz, J. A. Grass, M. E. Boyer, H. Guend, and E. H. Bresnick
Dynamic GATA Factor Interplay at a Multicomponent Regulatory Region of the GATA-2 Locus
J. Biol. Chem.,
January 21, 2005;
280(3):
1724 - 1732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Khandekar, N. Suzuki, J. Lewton, M. Yamamoto, and J. D. Engel
Multiple, Distant Gata2 Enhancers Specify Temporally and Tissue-Specific Patterning in the Developing Urogenital System
Mol. Cell. Biol.,
December 1, 2004;
24(23):
10263 - 10276.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-W. Ling, K. Ottersbach, J. P. van Hamburg, A. Oziemlak, F.-Y. Tsai, S. H. Orkin, R. Ploemacher, R. W. Hendriks, and E. Dzierzak
GATA-2 Plays Two Functionally Distinct Roles during the Ontogeny of Hematopoietic Stem Cells
J. Exp. Med.,
October 4, 2004;
200(7):
871 - 882.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Cairns, E. Moroni, E. Levantini, A. Giorgetti, F. G. Klinger, S. Ronzoni, L. Tatangelo, C. Tiveron, M. De Felici, S. Dolci, et al.
Kit regulatory elements required for expression in developing hematopoietic and germ cell lineages
Blood,
December 1, 2003;
102(12):
3954 - 3962.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Minegishi, N. Suzuki, T. Yokomizo, X. Pan, T. Fujimoto, S. Takahashi, T. Hara, A. Miyajima, S.-i. Nishikawa, and M. Yamamoto
Expression and domain-specific function of GATA-2 during differentiation of the hematopoietic precursor cells in midgestation mouse embryos
Blood,
August 1, 2003;
102(3):
896 - 905.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Grass, M. E. Boyer, S. Pal, J. Wu, M. J. Weiss, and E. H. Bresnick
GATA-1-dependent transcriptional repression of GATA-2 via disruption of positive autoregulation and domain-wide chromatin remodeling
PNAS,
July 22, 2003;
100(15):
8811 - 8816.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Oettgen
Transcriptional Regulation of Vascular Development
Circ. Res.,
August 31, 2001;
89(5):
380 - 388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Marshall, C. Kinnon, and A. J. Thrasher
Polarized expression of bone morphogenetic protein-4 in the human aorta-gonad-mesonephros region
Blood,
August 15, 2000;
96(4):
1591 - 1593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nishimura, S. Takahashi, T. Kuroha, N. Suwabe, T. Nagasawa, C. Trainor, and M. Yamamoto
A GATA Box in the GATA-1 Gene Hematopoietic Enhancer Is a Critical Element in the Network of GATA Factors and Sites That Regulate This Gene
Mol. Cell. Biol.,
January 15, 2000;
20(2):
713 - 723.
[Abstract]
[Full Text]
|
|
|
|
|
