|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 2 (January 15), 1999:
pp. 481-487
Human Growth Factor-Enhanced Regeneration of Transplantable Human
Hematopoietic Stem Cells in Nonobese Diabetic/Severe
Combined Immunodeficient Mice
Johanne D. Cashman and
Connie J. Eaves
From the Terry Fox Laboratory, British Columbia Cancer Agency and
Department of Medical Genetics, University of British Columbia,
Vancouver, BC, Canada.
Self-renewal is considered to be the essential defining property of
a stem cell. Retroviral marking, in vitro amplification, and serial
transplantation of human cells that can sustain long-term lymphomyelopoiesis in vivo have provided evidence that human
hematopoietic stem cell self-renewal occurs both in vitro and in vivo.
To investigate whether this process can be manipulated by cytokines, we
administered two different combinations of human growth factors to
sublethally irradiated nonobese diabetic/severe combined
immunodeficient (SCID) mice transplanted with
107 light-density human cord blood cells and then performed
secondary transplants to compare the number of transplantable human
lymphomyeloid reconstituting cells present 4 to 6 weeks
post-transplant. A 2-week course of Steel factor + interleukin (IL)-3 + granulocyte-macrophage colony-stimulating factor + erythropoietin
(3 times per week just before sacrifice) specifically and significantly
enhanced the numbers of transplantable human lymphomyeloid stem cells
detectable in the primary mice (by a factor of 10). Steel factor + Flt3-ligand + IL-6 (using either the same schedule or
administered daily until sacrifice 4 weeks post-transplant) gave a
threefold enhancement of this population. These effects were obtained
at a time when the regenerating human progenitor populations in such
primary mice are known to be maximally cycling even in the absence of growth factor administration suggesting that the underlying mechanism may reflect an ability of these growth factors to alter the probability of differentiation of stem cells stimulated to proliferate in vivo.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. P. Chute, G. G. Muramoto, A. B. Salter, S. K. Meadows, D. W. Rickman, B. Chen, H. A. Himburg, and N. J. Chao
Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice
Blood,
March 15, 2007;
109(6):
2365 - 2372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Puthenveetil, J. Scholes, D. Carbonell, N. Qureshi, P. Xia, L. Zeng, S. Li, Y. Yu, A. L Hiti, J.-K. Yee, et al.
Successful correction of the human {beta}-thalassemia major phenotype using a lentiviral vector
Blood,
December 1, 2004;
104(12):
3445 - 3453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Horn, B. M. Thomasson, B. L. Wood, R. G. Andrews, J. C. Morris, and H.-P. Kiem
Distinct hematopoietic stem/progenitor cell populations are responsible for repopulating NOD/SCID mice compared with nonhuman primates
Blood,
December 15, 2003;
102(13):
4329 - 4335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. Kalberer, U. Siegler, and A. Wodnar-Filipowicz
Human NK cell development in NOD/SCID mice receiving grafts of cord blood CD34+ cells
Blood,
July 1, 2003;
102(1):
127 - 135.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yahata, K. Ando, T. Sato, H. Miyatake, Y. Nakamura, Y. Muguruma, S. Kato, and T. Hotta
A highly sensitive strategy for SCID-repopulating cell assay by direct injection of primitive human hematopoietic cells into NOD/SCID mice bone marrow
Blood,
April 15, 2003;
101(8):
2905 - 2913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cashman, B. Dykstra, I. Clark-Lewis, A. Eaves, and C. Eaves
Changes in the Proliferative Activity of Human Hematopoietic Stem Cells in NOD/SCID Mice and Enhancement of Their Transplantability after In Vivo Treatment with Cell Cycle Inhibitors
J. Exp. Med.,
November 4, 2002;
196(9):
1141 - 1150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, M. Bhatia, H. H. H. Kanhai, R. Breese, D. K. Heilman, C. S. Johnson, J. H. F. Falkenburg, and E. F. Srour
Engraftment potential of human fetal hematopoietic cells in NOD/SCID mice is not restricted to mitotically quiescent cells
Blood,
June 17, 2002;
100(1):
120 - 127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Lotti, E. Menguzzato, C. Rossi, L. Naldini, L. Ailles, F. Mavilio, and G. Ferrari
Transcriptional Targeting of Lentiviral Vectors by Long Terminal Repeat Enhancer Replacement
J. Virol.,
March 19, 2002;
76(8):
3996 - 4007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. C. C. Kerre, G. De Smet, M. De Smedt, F. Offner, J. De Bosscher, J. Plum, and B. Vandekerckhove
Both CD34+38+ and CD34+38- Cells Home Specifically to the Bone Marrow of NOD/LtSZ scid/scid Mice but Show Different Kinetics in Expansion
J. Immunol.,
October 1, 2001;
167(7):
3692 - 3698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. D. Lewis, G. Almeida-Porada, J. Du, I. R. Lemischka, K. A. Moore, E. D. Zanjani, and C. M. Verfaillie
Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system
Blood,
June 1, 2001;
97(11):
3441 - 3449.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N.-B. Woods, C. Fahlman, H. Mikkola, I. Hamaguchi, K. Olsson, R. Zufferey, S. E. Jacobsen, D. Trono, and S. Karlsson
Lentiviral gene transfer into primary and secondary NOD/SCID repopulating cells
Blood,
December 1, 2000;
96(12):
3725 - 3733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Cashman and C. J. Eaves
High marrow seeding efficiency of human lymphomyeloid repopulating cells in irradiated NOD/SCID mice
Blood,
December 1, 2000;
96(12):
3979 - 3981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, J. H. F. Falkenburg, X. Tong, W. A. Noort, R. Breese, D. Heilman, H. Kanhai, C. M. Orschell-Traycoff, and E. F. Srour
Similar repopulating capacity of mitotically active and resting umbilical cord blood CD34+ cells in NOD/SCID mice
Blood,
September 15, 2000;
96(6):
2100 - 2107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. W. Zandstra, D. A. Lauffenburger, and C. J. Eaves
A ligand-receptor signaling threshold model of stem cell differentiation control: a biologically conserved mechanism applicable to hematopoiesis
Blood,
August 15, 2000;
96(4):
1215 - 1222.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Glimm and C.J. Eaves
Direct Evidence for Multiple Self-Renewal Divisions of Human In Vivo Repopulating Hematopoietic Cells in Short-Term Culture
Blood,
October 1, 1999;
94(7):
2161 - 2168.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
