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Blood, Vol. 93 No. 6 (March 15), 1999:
pp. 1916-1921
Hematopoietic Stem Cell Tracking In Vivo: A Comparison of
Short-Term and Long-Term Repopulating Cells
Sophie M. Lanzkron,
Michael I. Collector, and
Saul J. Sharkis
From the Johns Hopkins Oncology Center, Baltimore, MD.
We have previously demonstrated that we could separate long-term
repopulating stem cells from cells that provided radioprotection (short-term repopulating cells) on the basis of size and suggested that
this might be due to the quiescent nature of long-term repopulating cells. To further define the activity of these populations, we used a
dye (PKH26), which incorporates into the membrane of cells and is
equally distributed to daughter cells when they divide. We developed an
assay, which allowed us to retrieve PKH26+ long-term and
short-term repopulating cells in the hematopoietic tissues of the
recipients posttransplant. We were able to recover the labeled cells
and determine their cell cycle activity, as well as their ability to
reconstitute secondary lethally irradiated hosts in limiting dilution.
The results of our assay suggest that long-term repopulating cells are
quiescent in the bone marrow (BM) 48 hours after transplant. We were
able to detect only a few labeled cells in the peripheral blood
posttransplant and even though cells homed to both the spleen and BM,
more long-term repopulating cells homed to the marrow and only these
cells, which homed to the marrow, were capable of reconstituting
lethally irradiated secondary hosts long-term.
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