|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 6 (March 15), 1999:
pp. 2121-2127
Structural and Functional Consequences of Antigenic Modulation of Red
Blood Cells With Methoxypoly(Ethylene Glycol)
Kari L. Murad,
Kathleen L. Mahany,
Carlo Brugnara,
Frans A. Kuypers,
John W. Eaton, and
Mark D. Scott
From Albany Medical College, Albany, NY; Children's Hospital,
Boston, MA; Children's Hospital Oakland Research Institute, Oakland,
CA; and Baylor College of Medicine, Houston, TX.
We previously showed that the covalent modification of the red blood
cell (RBC) surface with methoxypoly(ethylene glycol) [mPEG; MW ~5
kD] could significantly attenuate the immunologic recognition of
surface antigens. However, to make these antigenically silent RBC a
clinically viable option, the mPEG-modified RBC must maintain normal
cellular structure and functions. To this end, mPEG-derivatization was
found to have no significant detrimental effects on RBC structure or
function at concentrations that effectively blocked antigenic
recognition of a variety of RBC antigens. Importantly, RBC lysis,
morphology, and hemoglobin oxidation state were unaffected by
mPEG-modification. Furthermore, as shown by functional studies of Band
3, a major site of modification, PEG-binding does not affect protein
function, as evidenced by normal SO4 flux.
Similarly, Na+ and K+ homeostasis were
unaffected. The functional aspects of the mPEG-modified RBC were also
maintained, as evidenced by normal oxygen binding and cellular
deformability. Perhaps most importantly, mPEG-derivatized mouse RBC
showed normal in vivo survival (~50 days) with no sensitization after
repeated transfusions. These data further support the hypothesis that
the covalent attachment of nonimmunogenic materials (eg, mPEG) to
intact RBC may have significant application in transfusion medicine,
especially for the chronically transfused and/or allosensitized patient.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. S. Narang and R. I. Mahato
Biological and biomaterial approaches for improved islet transplantation.
Pharmacol. Rev.,
June 1, 2006;
58(2):
194 - 243.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Faure, T. Hauet, Z. Han, J. M. Goujon, I. Petit, G. Mauco, M. Eugene, M. Carretier, and V. Papadopoulos
Polyethylene Glycol Reduces Early and Long-Term Cold Ischemia-Reperfusion and Renal Medulla Injury
J. Pharmacol. Exp. Ther.,
September 1, 2002;
302(3):
861 - 870.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Garratty, M. J. Telen, and L. D. Petz
Red Cell Antigens as Functional Molecules and Obstacles to Transfusion
Hematology,
January 1, 2002;
2002(1):
445 - 462.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Hauet, J. M. Goujon, and A. Vandewalle
To what extent can limiting cold ischaemia/reperfusion injury prevent delayed graft function?
Nephrol. Dial. Transplant.,
October 1, 2001;
16(10):
1982 - 1985.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. P. Blackall, J. K. Armstrong, H. J. Meiselman, and T. C. Fisher
Polyethylene glycol-coated red blood cells fail to bind glycophorin A-specific antibodies and are impervious to invasion by the Plasmodium falciparum malaria parasite
Blood,
January 15, 2001;
97(2):
551 - 556.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Murad, E. J. Gosselin, J. W. Eaton, and M. D. Scott
Stealth Cells: Prevention of Major Histocompatibility Complex Class II-Mediated T-Cell Activation by Cell Surface Modification
Blood,
September 15, 1999;
94(6):
2135 - 2141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
