|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 93 No. 7 (April 1), 1999:
pp. 2302-2307
Identification of Cord Blood Dendritic Cells as an Immature
CD11c Population
Rüdiger V. Sorg,
Gesine Kögler, and
Peter Wernet
From the Bone Marrow Donor Center with Transplantation Immunology and
EuroCord Bank Germany, Heinrich Heine University Medical Center,
Düsseldorf, Germany.
Dendritic cells (DC) are the main stimulators of primary T-cell
responses and, thus, probably play a role in the immune reactions after
stem cell transplantation. Very little is known about DC in cord blood
(CB) and about their potential involvement in the low incidence and
severity of acute graft-versus-host disease after CB transplantation.
Here, CBDC were identified as a HLA-DR+ cell population,
lacking the CD3, CD11b, CD14, CD16, CD19, CD34, CD56, and glycophorin A
lineage markers (lin). This lin /HLA-DR+
population represented 0.3% ± 0.1% (mean ± SD; range, 0.1% to 0.6%; n = 15) of CB mononuclear cells, and CB contained 5.4 ± 3.2 × 103 CBDC/mL (1.8 to
13.0 × 103; n = 15). CBDC expressed CD4, CD11a,
CD18, CD45RA, CD50, CD54, and CD123, but showed no expression of CD1a,
CD11c, CD33, CD40, CD45R0, CD80, CD83, and CD86 and only limited
expression of CD58, CD102, and CD116. Despite this immature phenotype,
immunomagnetically lin-enriched CBDC were potent
stimulators of allogeneic CB T cells. As few as 266 ± 107 (193 to
530; n = 10) lin/HLA-DR+ CBDC
stimulated a significant response. However, CBDC failed to take up
protein or peptide antigens. Thus, in CB there is a prevalence of a DC
subpopulation, resembling the CD11c DC identified in
tonsils, the so-called plasmacytoid T cells, which may exert a function
distinct from the CD11c+ DC subpopulation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Kobari, M. C. Giarratana, J. C. Gluckman, L. Douay, and M. Rosenzwajg
Ex Vivo Expansion Does Not Alter the Capacity of Umbilical Cord Blood CD34+ Cells to Generate Functional T Lymphocytes and Dendritic Cells
Stem Cells,
September 1, 2006;
24(9):
2150 - 2157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. Upham, A. Rate, J. Rowe, M. Kusel, P. D. Sly, and P. G. Holt
Dendritic Cell Immaturity during Infancy Restricts the Capacity To Express Vaccine-Specific T-Cell Memory
Infect. Immun.,
February 1, 2006;
74(2):
1106 - 1112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Mason, A. J. Stagg, S. C. Knight, and R. F. Lamont
The Measurement of Dendritic Cells in Umbilical Cord Blood: A Novel Technique Using Small Volumes of Whole Blood
Reproductive Sciences,
May 1, 2005;
12(4):
246 - 252.
[Abstract]
[PDF]
|
|
|
|
|
|
|
K. McKenna, A.-S. Beignon, and N. Bhardwaj
Plasmacytoid Dendritic Cells: Linking Innate and Adaptive Immunity
J. Virol.,
January 1, 2005;
79(1):
17 - 27.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Thornton, J. W. Upham, M. E. Wikstrom, B. J. Holt, G. P. White, M. J. Sharp, P. D. Sly, and P. G. Holt
Functional Maturation of CD4+CD25+CTLA4+CD45RA+ T Regulatory Cells in Human Neonatal T Cell Responses to Environmental Antigens/Allergens
J. Immunol.,
September 1, 2004;
173(5):
3084 - 3092.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Young, M. A. Simon, M. A. Baird, G. W. Tannock, R. Bibiloni, K. Spencely, J. M. Lane, P. Fitzharris, J. Crane, I. Town, et al.
Bifidobacterial Species Differentially Affect Expression of Cell Surface Markers and Cytokines of Dendritic Cells Harvested from Cord Blood
Clin. Vaccine Immunol.,
July 1, 2004;
11(4):
686 - 690.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Salio, M. J. Palmowski, A. Atzberger, I. F. Hermans, and V. Cerundolo
CpG-matured Murine Plasmacytoid Dendritic Cells Are Capable of In Vivo Priming of Functional CD8 T Cell Responses to Endogenous but Not Exogenous Antigens
J. Exp. Med.,
February 17, 2004;
199(4):
567 - 579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Szabolcs, K.-D. Park, M. Reese, L. Marti, G. Broadwater, and J. Kurtzberg
Absolute Values of Dendritic Cell Subsets in Bone Marrow, Cord Blood, and Peripheral Blood Enumerated by a Novel Method
Stem Cells,
May 1, 2003;
21(3):
296 - 303.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Schotte, M.-C. Rissoan, N. Bendriss-Vermare, J.-M. Bridon, T. Duhen, K. Weijer, F. Briere, and H. Spits
The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development
Blood,
February 1, 2003;
101(3):
1015 - 1023.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. H. Cottler-Fox, T. Lapidot, I. Petit, O. Kollet, J. F. DiPersio, D. Link, and S. Devine
Stem Cell Mobilization
Hematology,
January 1, 2003;
2003(1):
419 - 437.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. Upham, P. T. Lee, B. J. Holt, T. Heaton, S. L. Prescott, M. J. Sharp, P. D. Sly, and P. G. Holt
Development of Interleukin-12-Producing Capacity throughout Childhood
Infect. Immun.,
December 1, 2002;
70(12):
6583 - 6588.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. Syme, J. C. L. Spurrell, E. K. Amankwah, F. H. Y. Green, and C. H. Mody
Primary Dendritic Cells Phagocytose Cryptococcus neoformans via Mannose Receptors and Fc{gamma} Receptor II for Presentation to T Lymphocytes
Infect. Immun.,
November 1, 2002;
70(11):
5972 - 5981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-C. Rissoan, T. Duhen, J.-M. Bridon, N. Bendriss-Vermare, C. Peronne, B. d. S. Vis, F. Briere, and E. E. M. Bates
Subtractive hybridization reveals the expression of immunoglobulinlike transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells
Blood,
October 16, 2002;
100(9):
3295 - 3303.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Comeau, A.-R. Van der Vuurst de Vries, C. R. Maliszewski, and L. Galibert
CD123bright Plasmacytoid Predendritic Cells: Progenitors Undergoing Cell Fate Conversion?
J. Immunol.,
July 1, 2002;
169(1):
75 - 83.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Tzachanis, A. Berezovskaya, L. M. Nadler, and V. A. Boussiotis
Blockade of B7/CD28 in mixed lymphocyte reaction cultures results in the generation of alternatively activated macrophages, which suppress T-cell responses
Blood,
February 15, 2002;
99(4):
1465 - 1473.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. F. Lipscomb and B. J. Masten
Dendritic Cells: Immune Regulators in Health and Disease
Physiol Rev,
January 1, 2002;
82(1):
97 - 130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Dzionek, Y. Sohma, J. Nagafune, M. Cella, M. Colonna, F. Facchetti, G. Gunther, I. Johnston, A. Lanzavecchia, T. Nagasaka, et al.
BDCA-2, a Novel Plasmacytoid Dendritic Cell-specific Type II C-type Lectin, Mediates Antigen Capture and Is a Potent Inhibitor of Interferon {alpha}/{beta} Induction
J. Exp. Med.,
December 17, 2001;
194(12):
1823 - 1834.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. L. MILLER, G. L. CHEW, C. A. BELL, S. A. BIEDERMANN, M. AGGARWAL, P. L. KINNEY, W. Y. TSAI, R. M. WHYATT, F. P. PERERA, and J. G. FORD
Prenatal Exposure, Maternal Sensitization, and Sensitization In Utero To Indoor Allergens in an Inner-City Cohort
Am. J. Respir. Crit. Care Med.,
September 15, 2001;
164(6):
995 - 1001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Chaperot, N. Bendriss, O. Manches, R. Gressin, M. Maynadie, F. Trimoreau, H. Orfeuvre, B. Corront, J. Feuillard, J.-J. Sotto, et al.
Identification of a leukemic counterpart of the plasmacytoid dendritic cells
Blood,
May 15, 2001;
97(10):
3210 - 3217.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Vandenabeele, H. Hochrein, N. Mavaddat, K. Winkel, and K. Shortman
Human thymus contains 2 distinct dendritic cell populations
Blood,
March 15, 2001;
97(6):
1733 - 1741.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Spits, F. Couwenberg, A. Q. Bakker, K. Weijer, and C. H. Uittenbogaart
Id2 and Id3 Inhibit Development of CD34+ Stem Cells into Predendritic Cell (Pre-DC)2 but Not into Pre-DC1: Evidence for a Lymphoid Origin of Pre-DC2
J. Exp. Med.,
December 18, 2000;
192(12):
1775 - 1784.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Arpinati, C. L. Green, S. Heimfeld, J. E. Heuser, and C. Anasetti
Granulocyte-colony stimulating factor mobilizes T helper 2-inducing dendritic cells
Blood,
April 15, 2000;
95(8):
2484 - 2490.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
