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Blood, Vol. 93 No. 7 (April 1), 1999:
pp. 2302-2307
Identification of Cord Blood Dendritic Cells as an Immature
CD11c Population
Rüdiger V. Sorg,
Gesine Kögler, and
Peter Wernet
From the Bone Marrow Donor Center with Transplantation Immunology and
EuroCord Bank Germany, Heinrich Heine University Medical Center,
Düsseldorf, Germany.
Dendritic cells (DC) are the main stimulators of primary T-cell
responses and, thus, probably play a role in the immune reactions after
stem cell transplantation. Very little is known about DC in cord blood
(CB) and about their potential involvement in the low incidence and
severity of acute graft-versus-host disease after CB transplantation.
Here, CBDC were identified as a HLA-DR+ cell population,
lacking the CD3, CD11b, CD14, CD16, CD19, CD34, CD56, and glycophorin A
lineage markers (lin). This lin /HLA-DR+
population represented 0.3% ± 0.1% (mean ± SD; range, 0.1% to 0.6%; n = 15) of CB mononuclear cells, and CB contained 5.4 ± 3.2 × 103 CBDC/mL (1.8 to
13.0 × 103; n = 15). CBDC expressed CD4, CD11a,
CD18, CD45RA, CD50, CD54, and CD123, but showed no expression of CD1a,
CD11c, CD33, CD40, CD45R0, CD80, CD83, and CD86 and only limited
expression of CD58, CD102, and CD116. Despite this immature phenotype,
immunomagnetically lin-enriched CBDC were potent
stimulators of allogeneic CB T cells. As few as 266 ± 107 (193 to
530; n = 10) lin/HLA-DR+ CBDC
stimulated a significant response. However, CBDC failed to take up
protein or peptide antigens. Thus, in CB there is a prevalence of a DC
subpopulation, resembling the CD11c DC identified in
tonsils, the so-called plasmacytoid T cells, which may exert a function
distinct from the CD11c+ DC subpopulation.
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