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Blood, Vol. 93 No. 8 (April 15), 1999:
pp. 2730-2737
The Effect of Glucocorticoids on the Expression of L-Selectin on
Polymorphonuclear Leukocyte
Motohito Nakagawa,
Gregory P. Bondy,
Dan Waisman,
Diane Minshall,
James C. Hogg, and
Stephan F. van Eeden
From the Pulmonary Research Laboratory, University of British
Columbia, St Paul's Hospital, Vancouver, British Columbia, Canada; and
the Department of Neonatology, Carmel Medical Center, Faculty of
Medicine, Technion, Harif, Israel.
When active bone marrow release is induced by inflammatory stimuli,
it is associated with an increase in L-selectin expression on
circulating polymorphonuclear leukocyte (PMN). This contrasts sharply
with glucocorticoid-induced granulocytosis that is associated with
decreased L-selectin expression on PMN. The present study was designed
to determine if the reduced L-selectin expression observed after
glucocorticoid treatment is the result of suppression of L-selectin
synthesis in the bone marrow. New Zealand white rabbits treated with
dexamethasone (2.0 mg/kg, a single dose intravenously) were shown to
have decreased L-selectin expression on circulating PMN 12 to
24 hours after treatment (P < .01) with a return to baseline
levels by 48 hours. When dexamethasone was administered 48 hours after
the bone marrow PMN were pulse labeled with the thymidine analogue,
5'-bromo-2'-deoxyuridine (BrdU), L-selectin expression on
BrdU-labeled PMN released from the bone marrow was decreased (P < .01). Dexamethasone decreased L-selectin expression on
segmented PMN in the bone marrow (P < .05) but not on PMN
already in the circulation. We conclude that glucocorticoids decrease L-selectin expression on circulating PMN by downregulating L-selectin expression in the maturation pool of bone marrow and speculate that
this is an important glucocorticoid effect that influences the
recruitment of PMN into inflammatory sites.
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