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Blood, Vol. 93 No. 9 (May 1), 1999:
pp. 2907-2917
Subcellular and Cell-Cycle Expression Profiles of CDK-Inhibitors in
Normal Differentiating Myeloid Cells
B. Yaroslavskiy,
S. Watkins,
A.D. Donnenberg,
T.J. Patton, and
R.A. Steinman
From the Departments of Medicine and Cell Biology, University of
Pittsburgh School of Medicine, Pittsburgh, PA.
A central question in hematopoiesis is how cell-cycling behavior
changes during the emergence of the differentiated state. To further
understand what genetic regulators might couple proliferation status to
differentiation, we studied the expression of the cell-cycle inhibitors
p21 and p27 during the in vitro differentiation of normal
CD34+ blast cells along the myeloid lineage. We find p27
but not p21 to be expressed in freshly harvested resting
CD34+ cells. Thereafter, p21 levels peak concurrent with
cellular proliferation and then decline in expression as cells undergo
terminal differentiation. In contrast, p27 levels are fairly constant
but the subcellular localization of p27 changes from nuclear expression
to predominantly cytoplasmic expression and finally to perinuclear
localization at progressive stages of differentiation. This report
discusses the implications of these findings.
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