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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Blom, B. Articles by Spits, H. Search for Related Content PubMed PubMed Citation Articles by Blom, B. Articles by Spits, H. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 93 No. 9 (May 1), 1999: pp. 3033-3043 TCR Gene Rearrangements and Expression of the Pre-T Cell Receptor Complex During Human T-Cell Differentiation Bianca Blom, Martie C.M. Verschuren, Mirjam H.M. Heemskerk, Arjen Q. Bakker, Ellen J. van Gastel-Mol, Ingrid L.M. Wolvers-Tettero, Jacques J.M. van Dongen, and Hergen Spits From the Division of Immunology, The Netherlands Cancer Institute, Amsterdam; and the Department of Immunology, Erasmus University Rotterdam and University Hospital Rotterdam, Rotterdam, The Netherlands. Recent studies have identified several populations of progenitor cells in the human thymus. The hematopoietic precursor activity of these populations has been determined. The most primitive human thymocytes express high levels of CD34 and lack CD1a. These cells acquire CD1a and differentiate into CD4+CD8+ through CD3CD4+CD8 and CD3CD4+ CD8+ intermediate populations. The status of gene rearrangements in the various TCR loci, in particular of TCR and TCR, has not been analyzed in detail. In the present study we have determined the status of TCR gene rearrangements of early human postnatal thymocyte subpopulations by Southern blot analysis. Our results indicate that TCR rearrangements initiate in CD34+CD1a cells preceding those in the TCR and TCR loci that commence in CD34+CD1a+ cells. Furthermore, we have examined at which cellular stage TCR selection occurs in humans. We analyzed expression of cytoplasmic TCR and cell-surface CD3 on thymocytes that lack a mature TCR. In addition, we overexpressed a constitutive-active mutant of p56lckF505 by retrovirus-mediated gene transfer in sequential stages of T-cell development and analyzed the effect in a fetal thymic organ culture system. Evidence is presented that TCR selection in humans is initiated at the transition of the CD3CD4+CD8 into the CD4+CD8+ stage. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Baleydier, A.-V. Decouvelaere, J. Bergeron, P. Gaulard, D. Canioni, Y. Bertrand, S. Lepretre, B. Petit, H. Dombret, K. Beldjord, et al. 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Interferon-inducible protein 10, monokine induced by interferon gamma, and interferon-inducible T-cell alpha chemoattractant are produced by thymic epithelial cells and attract T-cell receptor (TCR) {alpha}{beta}+CD8+ single-positive T cells, TCR{gamma}{delta}+ T cells, and natural killer-type cells in human thymus Blood, February 1, 2001; 97(3): 601 - 607. [Abstract] [Full Text] [PDF] H. Spits, F. Couwenberg, A. Q. Bakker, K. Weijer, and C. H. Uittenbogaart Id2 and Id3 Inhibit Development of Cd34+ Stem Cells into Predendritic Cell (Pre-Dc)2 but Not into Pre-Dc1: Evidence for a Lymphoid Origin of Pre-Dc2 J. Exp. Med., December 18, 2000; 192(12): 1775 - 1784. [Abstract] [Full Text] [PDF] M. Yassai and J. Gorski Thymocyte Maturation: Selection for In-Frame TCR {alpha}-Chain Rearrangement Is Followed by Selection for Shorter TCR {beta}-Chain Complementarity-Determining Region 3 J. Immunol., October 1, 2000; 165(7): 3706 - 3712. [Abstract] [Full Text] [PDF] C. Brumpt, E. Delabesse, K. Beldjord, F. Davi, J.-M. Cayuela, C. Millien, P. Villarese, P. Quartier, A. Buzyn, F. Valensi, et al. The incidence of clonal T-cell receptor rearrangements in B-cell precursor acute lymphoblastic leukemia varies with age and genotype Blood, September 15, 2000; 96(6): 2254 - 2261. [Abstract] [Full Text] [PDF] Y. R. Carrasco, C. Trigueros, A. R. Ramiro, V. G. de Yebenes, and M. L. Toribio beta -Selection Is Associated With the Onset of CD8beta Chain Expression on CD4+CD8alpha alpha + Pre-T Cells During Human Intrathymic Development Blood, November 15, 1999; 94(10): 3491 - 3498. [Abstract] [Full Text] [PDF]

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