|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 1 (July 1), 1999:
pp. 62-73
Human CD34+ Cells Express CXCR4 and Its Ligand Stromal
Cell-Derived Factor-1. Implications for Infection by T-Cell Tropic
Human Immunodeficiency Virus
Alessandro Aiuti,
Lucia Turchetto,
Manuela Cota,
Arcadi Cipponi,
Andrea Brambilla,
Cinzia Arcelloni,
Rita Paroni,
Elisa Vicenzi,
Claudio Bordignon, and
Guido Poli
From Telethon Institute for Gene Therapy (TIGET); AIDS
Immunopathogenesis Unit, DIBIT; and Laboratory of Separative
Techniques, Scientific Institute H.S. Raffaele, Milan, Italy.
Human CD34+ hematopoietic progenitor cells obtained
from bone marrow (BM), umbilical cord blood (UCB), and mobilized
peripheral blood (MPB) were purified and investigated for the
expression of the chemokine receptor CXCR4 and its ligand, stromal
cell-derived factor-1 (SDF-1). CXCR4 was found present on the cell
surface of all CD34+ cells, although it was expressed at
lower density on MPB with respect to BM CD34+ cells.
Freshly isolated and in vitro-cultured CD34+ cells also
coexpressed SDF-1 mRNA, as determined by reverse
transcriptase-polymerase chain reaction (RT-PCR). Of interest,
CD34+/CD38+ committed progenitor cells,
unlike primitive CD34+/CD38 cells,
expressed SDF-1 mRNA. Supernatants from in vitro-cultured CD34+ cells contained substantial (3 to 8 ng/mL) amounts
of SDF-1 by enzyme-linked immunosorbent assay and induced migration of
CD34+ cells. Because CD34+ cells express
low levels of CD4, the primary receptor of the human immunodeficiency
virus (HIV), and CXCR4 is a coreceptor for T-cell tropic (X4) HIV
strains, we investigated the susceptibility of CD34+
cells to infection by this subset of viruses. Lack of productive infection was almost invariably observed as determined by a
conventional RT activity in culture supernatants and by real-time PCR
for HIV DNA in CD34+ cells exposed to both laboratory
adapted (LAI) and primary (BON) X4 T-cell tropic HIV-1 strain. Soluble
gp120 Env (sgp120) from X4 HIV-1 efficiently blocked binding of the
anti-CD4 Leu3a monoclonal antibody (MoAb) to either human
CD4+ T cells or CD34+ cells. In contrast,
sgp120 interfered with an anti-CXCR4 MoAb binding to human T
lymphocytes, but not to CD34+ cells. However, CXCR4 on
CD34+ cells was downregulated by SDF-1. These results
suggest that CXCR4 and its ligand SDF-1 expressed in
CD34+ progenitors may play an important role in
regulating the local and systemic trafficking of these cells. Moreover,
these findings suggest multiple and potentially synergistic mechanisms
at the basis of the resistance of CD34+ cells to X4 HIV
infection, including their ability to produce SDF-1, and the lack of
CXCR4 internalization following gp120 binding to CD4.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. D. Notarangelo and R. Badolato
Leukocyte trafficking in primary immunodeficiencies
J. Leukoc. Biol.,
March 1, 2009;
85(3):
335 - 343.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-C. Lee, H.-C. Hsu, W.-Y. I. Tseng, C.-Y. Chen, H.-J. Lin, Y.-L. Ho, M.-J. Su, and M.-F. Chen
Cell therapy generates a favourable chemokine gradient for stem cell recruitment into the infarcted heart in rabbits
Eur J Heart Fail,
March 1, 2009;
11(3):
238 - 245.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. Ghosh, D. Baatar, G. Collins, A. Carter, F. Indig, A. Biragyn, and D. D. Taub
Dexamethasone augments CXCR4-mediated signaling in resting human T cells via the activation of the Src kinase Lck
Blood,
January 15, 2009;
113(3):
575 - 584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-Y. Chen, M. Malik, B. E. Tomkowicz, R. G. Collman, and A. Ptasznik
BCR-ABL1 alters SDF-1{alpha}-mediated adhesive responses through the {beta}2 integrin LFA-1 in leukemia cells
Blood,
May 15, 2008;
111(10):
5182 - 5186.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Jin, Y. Tabe, S. Konoplev, Y. Xu, C. E. Leysath, H. Lu, S. Kimura, A. Ohsaka, M.-B. Rios, L. Calvert, et al.
CXCR4 up-regulation by imatinib induces chronic myelogenous leukemia (CML) cell migration to bone marrow stroma and promotes survival of quiescent CML cells
Mol. Cancer Ther.,
January 1, 2008;
7(1):
48 - 58.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. De La Luz Sierra, P. Gasperini, P. J. McCormick, J. Zhu, and G. Tosato
Transcription factor Gfi-1 induced by G-CSF is a negative regulator of CXCR4 in myeloid cells
Blood,
October 1, 2007;
110(7):
2276 - 2285.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Misao, G. Takemura, M. Arai, T. Ohno, H. Onogi, T. Takahashi, S. Minatoguchi, T. Fujiwara, and H. Fujiwara
Importance of recruitment of bone marrow-derived CXCR4+ cells in post-infarct cardiac repair mediated by G-CSF
Cardiovasc Res,
August 1, 2006;
71(3):
455 - 465.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Nakata, B. Tomkowicz, A. M. Gewirtz, and A. Ptasznik
Integrin inhibition through Lyn-dependent cross talk from CXCR4 chemokine receptors in normal human CD34+ marrow cells
Blood,
June 1, 2006;
107(11):
4234 - 4239.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tavor, I. Petit, S. Porozov, A. Avigdor, A. Dar, L. Leider-Trejo, N. Shemtov, V. Deutsch, E. Naparstek, A. Nagler, et al.
CXCR4 Regulates Migration and Development of Human Acute Myelogenous Leukemia Stem Cells in Transplanted NOD/SCID Mice
Cancer Res.,
April 15, 2004;
64(8):
2817 - 2824.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Kollet, I. Petit, J. Kahn, S. Samira, A. Dar, A. Peled, V. Deutsch, M. Gunetti, W. Piacibello, A. Nagler, et al.
Human CD34+CXCR4- sorted cells harbor intracellular CXCR4, which can be functionally expressed and provide NOD/SCID repopulation
Blood,
September 26, 2002;
100(8):
2778 - 2786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aiuti, S. Slavin, M. Aker, F. Ficara, S. Deola, A. Mortellaro, S. Morecki, G. Andolfi, A. Tabucchi, F. Carlucci, et al.
Correction of ADA-SCID by Stem Cell Gene Therapy Combined with Nonmyeloablative Conditioning
Science,
June 28, 2002;
296(5577):
2410 - 2413.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-J. Lataillade, D. Clay, P. Bourin, F. Herodin, C. Dupuy, C. Jasmin, and M.-C. Le Bousse-Kerdiles
Stromal cell-derived factor 1 regulates primitive hematopoiesis by suppressing apoptosis and by promoting G0/G1 transition in CD34+ cells: evidence for an autocrine/paracrine mechanism
Blood,
February 15, 2002;
99(4):
1117 - 1129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Thiebot, F. Louache, B. Vaslin, T. de Revel, O. Neildez, J. Larghero, W. Vainchenker, D. Dormont, and R. Le Grand
Early and Persistent Bone Marrow Hematopoiesis Defect in Simian/Human Immunodeficiency Virus-Infected Macaques despite Efficient Reduction of Viremia by Highly Active Antiretroviral Therapy during Primary Infection
J. Virol.,
December 1, 2001;
75(23):
11594 - 11602.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Majka, A. Janowska-Wieczorek, J. Ratajczak, K. Ehrenman, Z. Pietrzkowski, M. A. Kowalska, A. M. Gewirtz, S. G. Emerson, and M. Z. Ratajczak
Numerous growth factors, cytokines, and chemokines are secreted by human CD34+ cells, myeloblasts, erythroblasts, and megakaryoblasts and regulate normal hematopoiesis in an autocrine/paracrine manner
Blood,
May 15, 2001;
97(10):
3075 - 3085.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Kollet, A. Spiegel, A. Peled, I. Petit, T. Byk, R. Hershkoviz, E. Guetta, G. Barkai, A. Nagler, and T. Lapidot
Rapid and efficient homing of human CD34+CD38{-}/lowCXCR4+ stem and progenitor cells to the bone marrow and spleen of NOD/SCID and NOD/SCID/B2mnull mice
Blood,
May 15, 2001;
97(10):
3283 - 3291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Guerriero, G. Mattia, U. Testa, C. Chelucci, G. Macioce, I. Casella, P. Samoggia, C. Peschle, and H. J. Hassan
Stromal cell-derived factor 1{alpha} increases polyploidization of megakaryocytes generated by human hematopoietic progenitor cells
Blood,
May 1, 2001;
97(9):
2587 - 2595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shen, T. Cheng, I. Olszak, E. Garcia-Zepeda, Z. Lu, S. Herrmann, R. Fallon, A. D. Luster, and D. T. Scadden
CXCR-4 Desensitization Is Associated with Tissue Localization of Hemopoietic Progenitor Cells
J. Immunol.,
April 15, 2001;
166(8):
5027 - 5033.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Alfano, G. Vallanti, P. Biswas, C. Bovolenta, E. Vicenzi, B. Mantelli, T. Pushkarsky, R. Rappuoli, A. Lazzarin, M. Bukrinsky, et al.
The Binding Subunit of Pertussis Toxin Inhibits HIV Replication in Human Macrophages and Virus Expression in Chronically Infected Promonocytic U1 Cells
J. Immunol.,
February 1, 2001;
166(3):
1863 - 1870.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rosu-Myles, L. Gallacher, B. Murdoch, D. A. Hess, M. Keeney, D. Kelvin, L. Dale, S. S. G. Ferguson, D. Wu, F. Fellows, et al.
The human hematopoietic stem cell compartment is heterogeneous for CXCR4 expression
PNAS,
December 19, 2000;
97(26):
14626 - 14631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Jinquan, S. Quan, H. H. Jacobi, C. Jing, A. Millner, B. Jensen, H. O. Madsen, L. P. Ryder, A. Svejgaard, H.-J. Malling, et al.
CXC chemokine receptor 3 expression on CD34+ hematopoietic progenitors from human cord blood induced by granulocyte-macrophage colony-stimulating factor: chemotaxis and adhesion induced by its ligands, interferon gamma -inducible protein 10 and monokine induced by interferon gamma
Blood,
August 15, 2000;
96(4):
1230 - 1238.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Mirandola, P. Secchiero, S. Pierpaoli, G. Visani, L. Zamai, M. Vitale, S. Capitani, and G. Zauli
Infection of CD34+ hematopoietic progenitor cells by human herpesvirus 7 (HHV-7)
Blood,
July 1, 2000;
96(1):
126 - 131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Vicenzi, P. P. Bordignon, P. Biswas, A. Brambilla, C. Bovolenta, M. Cota, F. Sinigaglia, and G. Poli
Envelope-Dependent Restriction of Human Immunodeficiency Virus Type 1 Spreading in CD4+ T Lymphocytes: R5 but Not X4 Viruses Replicate in the Absence of T-Cell Receptor Restimulation
J. Virol.,
September 1, 1999;
73(9):
7515 - 7523.
[Abstract]
[Full Text]
|
|
|
|
|
