|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 11 (December 1), 1999:
pp. 3754-3763
SCL Expression in the Mouse Embryo Detected With a Targeted
lacZ Reporter Gene Demonstrates Its Localization to
Hematopoietic, Vascular, and Neural Tissues
Andrew G. Elefanty,
C. Glenn Begley,
Lynne Hartley,
Bette Papaevangeliou, and
Lorraine Robb
From The Walter and Eliza Hall Institute of Medical Research, the
Cooperative Research Centre for Cellular Growth Factors and the Rotary
Bone Marrow Research Laboratories, Victoria, Australia.
The helix-loop-helix transcription factor SCL (TAL1)
is indispensable for blood cell formation in the mouse embryo. We have explored the localization and developmental potential of cells fated to
express SCL during murine development using SCL-lacZ mutant mice in which the Escherichia coli lacZ
reporter gene was `knocked in' to the SCL locus. In addition
to the hematopoietic defect associated with SCL deficiency, the
yolk sac blood vessels in SCLlacZ/lacZ embryos
formed an abnormal primary vascular plexus, which failed to undergo
subsequent remodeling and formation of large branching vessels.
Intraembryonic vasculogenesis in precirculation
SCLlacZ/lacZ embryos appeared normal but, in
embryos older than embryonic day (E) 8.5 to E9, absolute anemia leading
to severe hypoxia precluded an accurate assessment of further vascular
development. In heterozygous SCLlacZ/w embryos,
lacZ was expressed in the central nervous system, vascular endothelia,
and primitive and definitive hematopoietic cells in the blood, aortic
wall, and fetal liver. Culture of fetal liver cells sorted for high and
low levels of  galactosidase activity from
SCLlacZ/w heterozygous embryos indicated that there
was a correlation between the level of SCL expression and the
frequency of hematopoietic progenitor cells.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Nie, H. Wu, and X.-H. Sun
Ubiquitination and Degradation of Tal1/SCL Are Induced by Notch Signaling and Depend on Skp2 and CHIP
J. Biol. Chem.,
January 11, 2008;
283(2):
684 - 692.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ogilvy, R. Ferreira, S. G. Piltz, J. M. Bowen, B. Gottgens, and A. R. Green
The SCL +40 Enhancer Targets the Midbrain Together with Primitive and Definitive Hematopoiesis and Is Regulated by SCL and GATA Proteins
Mol. Cell. Biol.,
October 15, 2007;
27(20):
7206 - 7219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J. Patterson, M. Gering, C. E. Eckfeldt, A. R. Green, C. M. Verfaillie, S. C. Ekker, and R. Patient
The transcription factors Scl and Lmo2 act together during development of the hemangioblast in zebrafish
Blood,
March 15, 2007;
109(6):
2389 - 2398.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Bockamp, C. Antunes, M. Maringer, R. Heck, K. Presser, S. Beilke, S. Ohngemach, R. Alt, M. Cross, R. Sprengel, et al.
Tetracycline-controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes, and c-kit-expressing lineage-negative hematopoietic cells
Blood,
September 1, 2006;
108(5):
1533 - 1541.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Reynaud, E. Ravet, M. Titeux, F. Mazurier, L. Renia, A. Dubart-Kupperschmitt, P.-H. Romeo, and F. Pflumio
SCL/TAL1 expression level regulates human hematopoietic stem cell self-renewal and engraftment
Blood,
October 1, 2005;
106(7):
2318 - 2328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Delabesse, S. Ogilvy, M. A. Chapman, S. G. Piltz, B. Gottgens, and A. R. Green
Transcriptional Regulation of the SCL Locus: Identification of an Enhancer That Targets the Primitive Erythroid Lineage In Vivo
Mol. Cell. Biol.,
June 15, 2005;
25(12):
5215 - 5225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. M. Schlaeger, H. K. A. Mikkola, C. Gekas, H. B. Helgadottir, and S. H. Orkin
Tie2Cre-mediated gene ablation defines the stem-cell leukemia gene (SCL/tal1)-dependent window during hematopoietic stem-cell development
Blood,
May 15, 2005;
105(10):
3871 - 3874.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J. Patterson, M. Gering, and R. Patient
Scl is required for dorsal aorta as well as blood formation in zebrafish embryos
Blood,
May 1, 2005;
105(9):
3502 - 3511.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Gothert, S. E. Gustin, M. A. Hall, A. R. Green, B. Gottgens, D. J. Izon, and C. G. Begley
In vivo fate-tracing studies using the Scl stem cell enhancer: embryonic hematopoietic stem cells significantly contribute to adult hematopoiesis
Blood,
April 1, 2005;
105(7):
2724 - 2732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Gothert, S. E. Gustin, J. A. M. van Eekelen, U. Schmidt, M. A. Hall, S. M. Jane, A. R. Green, B. Gottgens, D. J. Izon, and C. G. Begley
Genetically tagging endothelial cells in vivo: bone marrow-derived cells do not contribute to tumor endothelium
Blood,
September 15, 2004;
104(6):
1769 - 1777.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Chen, M. M.W. Chong, R. Darwiche, H. E. Thomas, and T. W.H. Kay
Severe Pancreatitis with Exocrine Destruction and Increased Islet Neogenesis in Mice with Suppressor of Cytokine Signaling-1 Deficiency
Am. J. Pathol.,
September 1, 2004;
165(3):
913 - 921.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Ravet, D. Reynaud, M. Titeux, B. Izac, S. Fichelson, P.-H. Romeo, A. Dubart-Kupperschmitt, and F. Pflumio
Characterization of DNA-binding-dependent and -independent functions of SCL/TAL1 during human erythropoiesis
Blood,
May 1, 2004;
103(9):
3326 - 3335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Gottgens, C. Broccardo, M.-J. Sanchez, S. Deveaux, G. Murphy, J. R. Gothert, E. Kotsopoulou, S. Kinston, L. Delaney, S. Piltz, et al.
The scl +18/19 Stem Cell Enhancer Is Not Required for Hematopoiesis: Identification of a 5' Bifunctional Hematopoietic-Endothelial Enhancer Bound by Fli-1 and Elf-1
Mol. Cell. Biol.,
March 1, 2004;
24(5):
1870 - 1883.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Lahlil, E. Lecuyer, S. Herblot, and T. Hoang
SCL Assembles a Multifactorial Complex That Determines Glycophorin A Expression
Mol. Cell. Biol.,
February 15, 2004;
24(4):
1439 - 1452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Martin, R. Lahlil, A. Damert, L. Miquerol, A. Nagy, G. Keller, and T. Hoang
SCL interacts with VEGF to suppress apoptosis at the onset of hematopoiesis
Development,
February 1, 2004;
131(3):
693 - 702.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gering, Y. Yamada, T. H. Rabbitts, and R. K. Patient
Lmo2 and Scl/Tal1 convert non-axial mesoderm into haemangioblasts which differentiate into endothelial cells in the absence of Gata1
Development,
December 22, 2003;
130(25):
6187 - 6199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Minegishi, N. Suzuki, T. Yokomizo, X. Pan, T. Fujimoto, S. Takahashi, T. Hara, A. Miyajima, S.-i. Nishikawa, and M. Yamamoto
Expression and domain-specific function of GATA-2 during differentiation of the hematopoietic precursor cells in midgestation mouse embryos
Blood,
August 1, 2003;
102(3):
896 - 905.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Byrd, S. Becker, P. Maye, R. Narasimhaiah, B. St-Jacques, X. Zhang, J. McMahon, A. McMahon, and L. Grabel
Hedgehog is required for murine yolk sac angiogenesis
Development,
March 3, 2003;
129(2):
361 - 372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ema, P. Faloon, W. J. Zhang, M. Hirashima, T. Reid, W. L. Stanford, S. Orkin, K. Choi, and J. Rossant
Combinatorial effects of Flk1 and Tal1 on vascular and hematopoietic development in the mouse
Genes & Dev.,
February 1, 2003;
17(3):
380 - 393.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. H. Hart, L. Hartley, K. Sourris, E. S. Stadler, R. Li, E. G. Stanley, P. P. L. Tam, A. G. Elefanty, and L. Robb
Mixl1 is required for axial mesendoderm morphogenesis and patterning in the murine embryo
Development,
August 1, 2002;
129(15):
3597 - 3608.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Sinclair, A. J. Bench, A. J. C. Bloor, J. Li, B. Gottgens, M. L. Stanley, J. Miller, S. Piltz, S. Hunter, E. P. Nacheva, et al.
Rescue of the lethal scl-/- phenotype by the human SCL locus
Blood,
May 13, 2002;
99(11):
3931 - 3938.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Gottgens, L. M. Barton, M. A. Chapman, A. M. Sinclair, B. Knudsen, D. Grafham, J. G.R. Gilbert, J. Rogers, D. R. Bentley, and A. R. Green
Transcriptional Regulation of the Stem Cell Leukemia Gene (SCL) --- Comparative Analysis of Five Vertebrate SCL Loci
Genome Res.,
May 1, 2002;
12(5):
749 - 759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. S. Chung, W. J. Zhang, E. Arentson, P. D. Kingsley, J. Palis, and K. Choi
Lineage analysis of the hemangioblast as defined by FLK1 and SCL expression
Development,
January 12, 2002;
129(23):
5511 - 5520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-J. Sanchez, E.-O. Bockamp, J. Miller, L. Gambardella, and A. R. Green
Selective rescue of early haematopoietic progenitors in Scl-/- mice by expressing Scl under the control of a stem cell enhancer
Development,
December 1, 2001;
128(23):
4815 - 4827.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. W. Roberts, L. Robb, S. Rakar, L. Hartley, L. Cluse, N. A. Nicola, D. Metcalf, D. J. Hilton, and W. S. Alexander
Placental defects and embryonic lethality in mice lacking suppressor of cytokine signaling 3
PNAS,
July 31, 2001;
98(16):
9324 - 9329.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
