|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 3 (August 1), 1999:
pp. 1121-1130
Regulation of p21rac Activation in Human Neutrophils
Niels Geijsen,
Sanne van Delft,
Jan A.M. Raaijmakers,
Jan-Willem
J. Lammers,
John G. Collard,
Leo Koenderman, and
Paul J. Coffer
From the Department of Pulmonary Diseases, University Hospital
Utrecht, Utrecht; and The Netherlands Cancer Institute, Division of
Cell Biology, Amsterdam, The Netherlands.
The small guanosine triphosphate (GTPase) p21rac is highly expressed
in human neutrophils where it is thought to play a role in cytoskeletal
reorganization and superoxide production. Using the p21rac binding
domain of PAK (PAK-RBD) as an activation-specific probe, we have
investigated agonist-stimulated activation of p21rac. Stimulation of
neutrophils with the chemoattractants fMet-Leu-Phe (fMLP) or
platelet-activating factor (PAF) induced an extremely rapid and
transient p21rac activation, being optimal within 5 seconds. This
activation correlates with the rapid changes of intracellular free
Ca2+ ([Ca2+]i) stimulated by
fMLP; however, changes in [Ca2+]i were
neither sufficient nor required for p21rac activation. Furthermore,
fMLP-induced p21rac activation was not inhibited by broad tyrosine
kinase inhibitors or specific inhibitors of ERK, p38 mitogen activated
protein kinase, Src, or phosphatidylinositol 3-kinases. Surprisingly,
the cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF)
and tumor necrosis factor- did not cause p21rac activation or
modulate fMLP-induced p21rac activation. AlF , a potent
activator of heterotrimeric G-protein -subunits, however, was found
to activate p21rac. Stimulation of neutrophils with phorbol myristate
acetate (PMA) strongly activated the respiratory burst,
but did not induce p21rac activation, suggesting that superoxide production per se can occur independently of p21rac activation. These
data suggest that in human granulocytes, G-protein coupled receptors,
but not cytokine receptors, activate p21rac via a rapid, novel
exchange-mechanism independently of changes in
[Ca2+]i, tyrosine phosphorylation, or PI3K.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Brechard and E. J. Tschirhart
Regulation of superoxide production in neutrophils: role of calcium influx
J. Leukoc. Biol.,
November 1, 2008;
84(5):
1223 - 1237.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Mitchell, A. Lo, M. R. Logan, P. Lacy, and G. Eitzen
Primary granule exocytosis in human neutrophils is regulated by Rac-dependent actin remodeling
Am J Physiol Cell Physiol,
November 1, 2008;
295(5):
C1354 - C1365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Kuijk, J. M. Beekman, J. Koster, H. R. Waterham, J. Frenkel, and P. J. Coffer
HMG-CoA reductase inhibition induces IL-1{beta} release through Rac1/PI3K/PKB-dependent caspase-1 activation
Blood,
November 1, 2008;
112(9):
3563 - 3573.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. El Bekay, G. Alba, M E. Reyes, P. Chacon, A. Vega, J. Martin-Nieto, J. Jimenez, E. Ramos, J. Olivan, E. Pintado, et al.
Rac2 GTPase activation by angiotensin II is modulated by Ca2+/calcineurin and mitogen-activated protein kinases in human neutrophils
J. Mol. Endocrinol.,
November 1, 2007;
39(5):
351 - 363.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Jin, R. M. Ray, and L. R. Johnson
Rac1 mediates intestinal epithelial cell apoptosis via JNK
Am J Physiol Gastrointest Liver Physiol,
December 1, 2006;
291(6):
G1137 - G1147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. M.-C. Dang, C. Elbim, J.-C. Marie, M. Chiandotto, M.-A. Gougerot-Pocidalo, and J. El-Benna
Anti-inflammatory effect of interleukin-10 on human neutrophil respiratory burst involves inhibition of GM-CSF-induced p47PHOX phosphorylation through a decrease in ERK1/2 activity
FASEB J,
July 1, 2006;
20(9):
1504 - 1506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. Brown, D. P. Del Re, and M. A. Sussman
The Rac and Rho Hall of Fame: A Decade of Hypertrophic Signaling Hits
Circ. Res.,
March 31, 2006;
98(6):
730 - 742.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Verploegen, L. Ulfman, H. W. M. van Deutekom, C. van Aalst, H. Honing, J.-W. J. Lammers, L. Koenderman, and P. J. Coffer
Characterization of the role of CaMKI-like kinase (CKLiK) in human granulocyte function
Blood,
August 1, 2005;
106(3):
1076 - 1083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Nijhara, P. B. van Hennik, M. L Gignac, M. J. Kruhlak, P. L. Hordijk, J. Delon, and S. Shaw
Rac1 Mediates Collapse of Microvilli on Chemokine-Activated T Lymphocytes
J. Immunol.,
October 15, 2004;
173(8):
4985 - 4993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Weiss-Haljiti, C. Pasquali, H. Ji, C. Gillieron, C. Chabert, M.-L. Curchod, E. Hirsch, A. J. Ridley, R. H. van Huijsduijnen, M. Camps, et al.
Involvement of Phosphoinositide 3-Kinase {gamma}, Rac, and PAK Signaling in Chemokine-induced Macrophage Migration
J. Biol. Chem.,
October 8, 2004;
279(41):
43273 - 43284.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nigorikawa, N. Okamura, and O. Hazeki
The Effect of Anionic Amphiphiles on the Recruitment of Rac in Neutrophils
J. Biochem.,
October 1, 2004;
136(4):
463 - 470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Fessler, P. G. Arndt, S. C. Frasch, J. G. Lieber, C. A. Johnson, R. C. Murphy, J. A. Nick, D. L. Bratton, K. C. Malcolm, and G. S. Worthen
Lipid Rafts Regulate Lipopolysaccharide-induced Activation of Cdc42 and Inflammatory Functions of the Human Neutrophil
J. Biol. Chem.,
September 17, 2004;
279(38):
39989 - 39998.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Abdel-Latif, M. Steward, D. L. Macdonald, G. A. Francis, M. C. Dinauer, and P. Lacy
Rac2 is critical for neutrophil primary granule exocytosis
Blood,
August 1, 2004;
104(3):
832 - 839.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Bayer, S. R. Halsell, J. W. Fristrom, D. P. Kiehart, and L. von Kalm
Genetic Interactions Between the RhoA and Stubble-stubbloid Loci Suggest a Role for a Type II Transmembrane Serine Protease in Intracellular Signaling During Drosophila Imaginal Disc Morphogenesis
Genetics,
November 1, 2003;
165(3):
1417 - 1432.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. S. Price, M. Langeslag, J. P. t. Klooster, P. L. Hordijk, K. Jalink, and J. G. Collard
Calcium Signaling Regulates Translocation and Activation of Rac
J. Biol. Chem.,
October 10, 2003;
278(41):
39413 - 39421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Forsberg, P. Druid, L. Zheng, O. Stendahl, and E. Sarndahl
Activation of Rac2 and Cdc42 on Fc and complement receptor ligation in human neutrophils
J. Leukoc. Biol.,
October 1, 2003;
74(4):
611 - 619.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Chodniewicz and D. V. Zhelev
Novel pathways of F-actin polymerization in the human neutrophil
Blood,
September 15, 2003;
102(6):
2251 - 2258.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Dib, F. Melander, L. Axelsson, M.-C. Dagher, P. Aspenstrom, and T. Andersson
Down-regulation of Rac Activity during {beta}2 Integrin-mediated Adhesion of Human Neutrophils
J. Biol. Chem.,
June 20, 2003;
278(26):
24181 - 24188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Wojciak-Stothard and A. J. Ridley
Shear stress-induced endothelial cell polarization is mediated by Rho and Rac but not Cdc42 or PI 3-kinases
J. Cell Biol.,
April 28, 2003;
161(2):
429 - 439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Li, A. Yamauchi, C. C. Marchal, J. K. Molitoris, L. A. Quilliam, and M. C. Dinauer
Chemoattractant-Stimulated Rac Activation in Wild-Type and Rac2-Deficient Murine Neutrophils: Preferential Activation of Rac2 and Rac2 Gene Dosage Effect on Neutrophil Functions
J. Immunol.,
November 1, 2002;
169(9):
5043 - 5051.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Vilhardt, O. Plastre, M. Sawada, K. Suzuki, M. Wiznerowicz, E. Kiyokawa, D. Trono, and K.-H. Krause
The HIV-1 Nef Protein and Phagocyte NADPH Oxidase Activation
J. Biol. Chem.,
October 25, 2002;
277(44):
42136 - 42143.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Grill and J. W. Schrader
Activation of Rac-1, Rac-2, and Cdc42 by hemopoietic growth factors or cross-linking of the B-lymphocyte receptor for antigen
Blood,
October 16, 2002;
100(9):
3183 - 3192.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Betson, E. Lozano, J. Zhang, and V. M. M. Braga
Rac Activation upon Cell-Cell Contact Formation Is Dependent on Signaling from the Epidermal Growth Factor Receptor
J. Biol. Chem.,
September 27, 2002;
277(40):
36962 - 36969.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M Pelletier and D Girard
Dieldrin induces human neutrophil superoxide production via protein kinases C and tyrosine kinases
Human and Experimental Toxicology,
August 1, 2002;
21(8):
415 - 420.
[Abstract]
[PDF]
|
|
|
|
|
|
|
H. Kanazawa, K. Ohsawa, Y. Sasaki, S. Kohsaka, and Y. Imai
Macrophage/Microglia-specific Protein Iba1 Enhances Membrane Ruffling and Rac Activation via Phospholipase C-gamma -dependent Pathway
J. Biol. Chem.,
May 24, 2002;
277(22):
20026 - 20032.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. van den Eijnde, M. J. B. van den Hoff, C. P. M. Reutelingsperger, W. L. van Heerde, M. E. R. Henfling, C. Vermeij-Keers, B. Schutte, M. Borgers, and F. C. S. Ramaekers
Transient expression of phosphatidylserine at cell-cell contact areas is required for myotube formation
J. Cell Sci.,
March 12, 2002;
114(20):
3631 - 3642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Nijhuis, J.-W. J Lammers, L. Koenderman, and P. J. Coffer
Src kinases regulate PKB activation and modulate cytokine and chemoattractant-controlled neutrophil functioning
J. Leukoc. Biol.,
January 1, 2002;
71(1):
115 - 124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-P. Gratacap, B. Payrastre, B. Nieswandt, and S. Offermanns
Differential Regulation of Rho and Rac through Heterotrimeric G-proteins and Cyclic Nucleotides
J. Biol. Chem.,
December 14, 2001;
276(51):
47906 - 47913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Adachi, R. Vita, S. Sannohe, S. Stafford, R. Alam, H. Kayaba, and J. Chihara
The Functional Role of Rho and Rho-Associated Coiled-Coil Forming Protein Kinase in Eotaxin Signaling of Eosinophils
J. Immunol.,
October 15, 2001;
167(8):
4609 - 4615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Chinnaiyan, M. Huber-Lang, C. Kumar-Sinha, T. R. Barrette, S. Shankar-Sinha, V. J. Sarma, V. A. Padgaonkar, and P. A. Ward
Molecular Signatures of Sepsis : Multiorgan Gene Expression Profiles of Systemic Inflammation
Am. J. Pathol.,
October 1, 2001;
159(4):
1199 - 1209.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Alblas, L. Ulfman, P. Hordijk, and L. Koenderman
Activation of RhoA and ROCK Are Essential for Detachment of Migrating Leukocytes
Mol. Biol. Cell,
July 1, 2001;
12(7):
2137 - 2145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. P. van Nieuw Amerongen and V. W.M. van Hinsbergh
Cytoskeletal Effects of Rho-Like Small Guanine Nucleotide-Binding Proteins in the Vascular System
Arterioscler Thromb Vasc Biol,
March 1, 2001;
21(3):
300 - 311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Lian, L. Crossley, Q. Zhan, R. Huang, P. Coffer, A. Toker, D. Robinson, and J. A. Badwey
Antagonists of Calcium Fluxes and Calmodulin Block Activation of the p21-Activated Protein Kinases in Neutrophils
J. Immunol.,
February 15, 2001;
166(4):
2643 - 2650.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Jefferies and L. A. J. O'Neill
Rac1 Regulates Interleukin 1-induced Nuclear Factor kappa B Activation in an Inhibitory Protein kappa Balpha -independent Manner by Enhancing the Ability of the p65 Subunit to Transactivate Gene Expression
J. Biol. Chem.,
February 4, 2000;
275(5):
3114 - 3120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Lian, P. G. Marks, J. Y. Wang, D. L. Falls, and J. A. Badwey
A Protein Kinase from Neutrophils That Specifically Recognizes Ser-3 in Cofilin
J. Biol. Chem.,
January 28, 2000;
275(4):
2869 - 2876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Lewis, C. Di Ciano, O. D. Rotstein, and A. Kapus
Osmotic stress activates Rac and Cdc42 in neutrophils: role in hypertonicity-induced actin polymerization
Am J Physiol Cell Physiol,
February 1, 2002;
282(2):
C271 - C279.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
