|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 4 (August 15), 1999:
pp. 1372-1381
Induction of Epstein-Barr Virus-Specific Cytotoxic T-Lymphocyte
Responses Using Dendritic Cells Pulsed With EBNA-3A
Peptides or UV-Inactivated, Recombinant EBNA-3A Vaccinia Virus
Marion Subklewe,
Ann Chahroudi,
Alan Schmaljohn,
Michael G. Kurilla,
Nina Bhardwaj, and
Ralph M. Steinman
From the Laboratory of Cellular Physiology and Immunology,
Rockefeller University, New York, NY; the Virology Division,
USAMRIID, Fort Detrick, Frederick, MD; and the Department of
Pathology and Microbiology, University of Virginia, Charlottesville,
VA.
Cell-mediated immunity, especially the cytotoxic T lymphocyte (CTL),
provides resistance to Epstein-Barr virus (EBV), as is demonstrated by
the occurrence of posttransplant lymphoproliferative disease in
immunosuppressed patients. We set out to use dendritic cells (DCs) to
elicit anti-EBV-specific CTLs in culture. In unselected, HLA-B8+ donors, monocyte-derived mature DCs were pulsed
with the HLA-B8-restricted EBNA-3A peptide, FLRGRAYGL, and added to
autologous T cells for 7 days at a DC:T ratio of 1:5 to 1:60. The
cultured cells specifically lysed EBNA-3A peptide-pulsed,
HLA-B8+, B-lymphoblastoid cell lines in a 5-hour
51Cr-release assay. The generation of CTLs did not require
the addition of interleukin-2. In comparison, monocytes were weak
antigen-presenting cells. DCs were then infected with recombinant
vaccinia-EBNA-3A. Vaccinia infection significantly decreased the
viability of immature DCs after 3 days of culture (to 25% to 45%) but
had a smaller effect on mature DC recovery (40% to 70%). To decrease
these cytopathic effects and to expand the potential use of vaccinia
vectors for DC therapy in immunocompromised patients, we successfully
used psoralen and UV-inactivated virus. Mature DCs pulsed with either live or inactivated vaccinia EBNA-3A virus could elicit strong EBNA-3A-specific CTLs. Therefore, mature DCs are powerful stimulators of EBV-specific CTLs and their major histocompatibility
complex class I products can even be charged with
UV-inactivated recombinant vaccinia.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Chahroudi, D. A. Garber, P. Reeves, L. Liu, D. Kalman, and M. B. Feinberg
Differences and similarities in viral life cycle progression and host cell physiology after infection of human dendritic cells with modified vaccinia virus ankara and vaccinia virus.
J. Virol.,
September 1, 2006;
80(17):
8469 - 8481.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Chahroudi, R. Chavan, N. Koyzr, E. K. Waller, G. Silvestri, and M. B. Feinberg
Vaccinia Virus Tropism for Primary Hematolymphoid Cells Is Determined by Restricted Expression of a Unique Virus Receptor
J. Virol.,
August 15, 2005;
79(16):
10397 - 10407.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. E. Whitelegg, L. E. M. Oosten, S. Jordan, M. Kester, A. G. S. van Halteren, J. A. Madrigal, E. Goulmy, and L. D. Barber
Investigation of Peptide Involvement in T Cell Allorecognition Using Recombinant HLA Class I Multimers
J. Immunol.,
August 1, 2005;
175(3):
1706 - 1714.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Chen, J. Hou, X. Jiang, S. Ma, M. Meng, B. Wang, M. Zhang, M. Zhang, X. Tang, F. Zhang, et al.
Response of Memory CD8+ T Cells to Severe Acute Respiratory Syndrome (SARS) Coronavirus in Recovered SARS Patients and Healthy Individuals
J. Immunol.,
July 1, 2005;
175(1):
591 - 598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Wang, H. Chen, X. Jiang, M. Zhang, T. Wan, N. Li, X. Zhou, Y. Wu, F. Yang, Y. Yu, et al.
Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein
Blood,
July 1, 2004;
104(1):
200 - 206.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Bickham, K. Goodman, C. Paludan, S. Nikiforow, M. L. Tsang, R. M. Steinman, and C. Munz
Dendritic Cells Initiate Immune Control of Epstein-Barr Virus Transformation of B Lymphocytes In Vitro
J. Exp. Med.,
December 1, 2003;
198(11):
1653 - 1663.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Santodonato, G. D'Agostino, R. Nisini, S. Mariotti, D. M. Monque, M. Spada, L. Lattanzi, M. Paola Perrone, M. Andreotti, F. Belardelli, et al.
Monocyte-Derived Dendritic Cells Generated After a Short-Term Culture with IFN-{alpha} and Granulocyte-Macrophage Colony-Stimulating Factor Stimulate a Potent Epstein-Barr Virus-Specific CD8+ T Cell Response
J. Immunol.,
May 15, 2003;
170(10):
5195 - 5202.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Y. Tsang, M. Zhu, J. Even, J. Gulley, P. Arlen, and J. Schlom
The Infection of Human Dendritic Cells with Recombinant Avipox Vectors Expressing a Costimulatory Molecule Transgene (CD80) to Enhance the Activation of Antigen-specific Cytolytic T Cells
Cancer Res.,
October 1, 2001;
61(20):
7568 - 7576.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. George-Chandy, N. Mielcarek, I. Nordstrom, J. Holmgren, and K. Eriksson
Vaccination with Bordetella pertussis-Pulsed Autologous or Heterologous Dendritic Cells Induces a Mucosal Antibody Response In Vivo and Protects against Infection
Infect. Immun.,
June 1, 2001;
69(6):
4120 - 4124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Haque, J. W. Hawes, and J. S. Blum
Cysteinylation of MHC Class II Ligands: Peptide Endocytosis and Reduction Within APC Influences T Cell Recognition
J. Immunol.,
April 1, 2001;
166(7):
4543 - 4551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Subklewe, C. Paludan, M. L. Tsang, K. Mahnke, R. M. Steinman, and C. Munz
Dendritic Cells Cross-Present Latency Gene Products from Epstein-Barr Virus-Transformed B Cells and Expand Tumor-Reactive Cd8+ Killer T Cells
J. Exp. Med.,
February 5, 2001;
193(3):
405 - 412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Krishnan, S. Sad, G. B. Patel, and G. D. Sprott
The Potent Adjuvant Activity of Archaeosomes Correlates to the Recruitment and Activation of Macrophages and Dendritic Cells In Vivo
J. Immunol.,
February 1, 2001;
166(3):
1885 - 1893.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Dyall, J.-B. Latouche, S. Schnell, and M. Sadelain
Lentivirus-transduced human monocyte-derived dendritic cells efficiently stimulate antigen-specific cytotoxic T lymphocytes
Blood,
January 1, 2001;
97(1):
114 - 121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. V. Dhodapkar, J. W. Young, P. B. Chapman, W. I. Cox, J. F. Fonteneau, S. Amigorena, A. N. Houghton, R. M. Steinman, and N. Bhardwaj
Paucity of Functional T-Cell Memory to Melanoma Antigens in Healthy Donors and Melanoma Patients
Clin. Cancer Res.,
December 1, 2000;
6(12):
4831 - 4838.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Granelli-Piperno, L. Zhong, P. Haslett, J. Jacobson, and R. M. Steinman
Dendritic Cells, Infected with Vesicular Stomatitis Virus-Pseudotyped HIV-1, Present Viral Antigens to CD4+ and CD8+ T Cells from HIV-1-Infected Individuals
J. Immunol.,
December 1, 2000;
165(11):
6620 - 6626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Krishnan, S. Sad, G. B. Patel, and G. D. Sprott
Archaeosomes Induce Long-Term CD8+ Cytotoxic T Cell Response to Entrapped Soluble Protein by the Exogenous Cytosolic Pathway, in the Absence of CD4+ T Cell Help
J. Immunol.,
November 1, 2000;
165(9):
5177 - 5185.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Weissman, H. Ni, D. Scales, A. Dude, J. Capodici, K. McGibney, A. Abdool, S. N. Isaacs, G. Cannon, and K. Kariko
HIV Gag mRNA Transfection of Dendritic Cells (DC) Delivers Encoded Antigen to MHC Class I and II Molecules, Causes DC Maturation, and Induces a Potent Human In Vitro Primary Immune Response
J. Immunol.,
October 15, 2000;
165(8):
4710 - 4717.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Gruber, J. Kan-Mitchell, K. L. Kuhen, T. Mukai, and F. Wong-Staal
Dendritic cells transduced by multiply deleted HIV-1 vectors exhibit normal phenotypes and functions and elicit an HIV-specific cytotoxic T-lymphocyte response in vitro
Blood,
August 15, 2000;
96(4):
1327 - 1333.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. Hodge, A. N. Rad, D. W. Grosenbach, H. Sabzevari, A. G. Yafal, L. Gritz, and J. Schlom
Enhanced Activation of T Cells by Dendritic Cells Engineered to Hyperexpress a Triad of Costimulatory Molecules
J Natl Cancer Inst,
August 2, 2000;
92(15):
1228 - 1239.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Munz, K. L. Bickham, M. Subklewe, M. L. Tsang, A. Chahroudi, M. G. Kurilla, D. Zhang, M. O'Donnell, and R. M. Steinman
Human Cd4+ T Lymphocytes Consistently Respond to the Latent Epstein-Barr Virus Nuclear Antigen Ebna1
J. Exp. Med.,
May 15, 2000;
191(10):
1649 - 1660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Engelmayer, M. Larsson, M. Subklewe, A. Chahroudi, W. I. Cox, R. M. Steinman, and N. Bhardwaj
Vaccinia Virus Inhibits the Maturation of Human Dendritic Cells: A Novel Mechanism of Immune Evasion
J. Immunol.,
December 15, 1999;
163(12):
6762 - 6768.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
