|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 94 No. 7 (October 1), 1999:
pp. 2515-2522
Homing of Human Cells in the Fetal Sheep Model: Modulation by
Antibodies Activating or Inhibiting Very Late Activation
Antigen-4-Dependent Function
Esmail D. Zanjani,
Alan W. Flake,
Graça Almeida-Porada,
Nam Tran, and
Thalia Papayannopoulou
From the VA Medical Center, Reno, NV; Children's Hospital of
Philadelphia, Philadelphia, PA; and the University of Washington,
Seattle, WA.
The mechanisms by which intravenously (IV)-administered
hematopoietic cells home to the bone marrow (BM) are poorly defined. Although insightful information has been obtained in mice, our knowledge about homing of human cells is very limited. In the present
study, we investigated the importance of very late activation antigen
(VLA)-4 in the early phases of lodgment of human CD34+
progenitors into the sheep hematopoietic compartment after in utero
transplantation. We have found that preincubation of donor cells with
anti-VLA-4 blocking antibodies resulted in a profound reduction of
human cell lodgment in the fetal BM at 24 and 48 hours after
transplantation, with a corresponding increase of human cells in the
peripheral circulation. Furthermore, IV infusion of the anti-VLA-4
antibody at later times (posttransplantation days 21 to 24) resulted in
redistribution or mobilization of human progenitors from the BM to the
peripheral blood. In an attempt to positively modulate homing, we also
pretreated human donor cells with an activating antibody to  1
integrins. This treatment resulted in increased lodgment of donor cells
in the fetal liver, presumably for hemodynamic reasons, at the expense
of the BM. Given previous involvement of the VLA-4/vascular cell
adhesion molecule (VCAM)-1 adhesion pathway in homing and
mobilization in the murine system, our present data suggest that
cross-reacting ligands (likely VCAM-1) for human VLA-4 exist in sheep
BM, thereby implicating conservation of molecular mechanisms of homing
and mobilization across disparate species barriers. Thus, information from xenogeneic models of human hematopoiesis and specifically, the
human/sheep model of in utero transplantation, may provide valuable
insights into human hematopoietic transplantation biology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Kumar and S. Ponnazhagan
Bone homing of mesenchymal stem cells by ectopic {alpha}4 integrin expression
FASEB J,
December 1, 2007;
21(14):
3917 - 3927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. H. Peranteau, M. Endo, O. O. Adibe, A. Merchant, P. W. Zoltick, and A. W. Flake
CD26 inhibition enhances allogeneic donor-cell homing and engraftment after in utero hematopoietic-cell transplantation
Blood,
December 15, 2006;
108(13):
4268 - 4274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Langer, A. E. May, K. Daub, U. Heinzmann, P. Lang, M. Schumm, D. Vestweber, S. Massberg, T. Schonberger, I. Pfisterer, et al.
Adherent Platelets Recruit and Induce Differentiation of Murine Embryonic Endothelial Progenitor Cells to Mature Endothelial Cells In Vitro
Circ. Res.,
February 3, 2006;
98(2):
e2 - e10.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Lapidot, A. Dar, and O. Kollet
How do stem cells find their way home?
Blood,
September 15, 2005;
106(6):
1901 - 1910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Scott, G. V. Priestley, and T. Papayannopoulou
Deletion of {alpha}4 Integrins from Adult Hematopoietic Cells Reveals Roles in Homeostasis, Regeneration, and Homing
Mol. Cell. Biol.,
December 15, 2003;
23(24):
9349 - 9360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Plett, S. M. Frankovitz, and C. M. Orschell
Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation
Blood,
September 15, 2003;
102(6):
2285 - 2291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yahata, K. Ando, T. Sato, H. Miyatake, Y. Nakamura, Y. Muguruma, S. Kato, and T. Hotta
A highly sensitive strategy for SCID-repopulating cell assay by direct injection of primitive human hematopoietic cells into NOD/SCID mice bone marrow
Blood,
April 15, 2003;
101(8):
2905 - 2913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Bug, T. Rossmanith, R. Henschler, L.A. Kunz-Schughart, B. Schroder, M. Kampfmann, M. Kreutz, D. Hoelzer, and O. G. Ottmann
Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells
J. Leukoc. Biol.,
October 1, 2002;
72(4):
837 - 845.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Kollet, I. Petit, J. Kahn, S. Samira, A. Dar, A. Peled, V. Deutsch, M. Gunetti, W. Piacibello, A. Nagler, et al.
Human CD34+CXCR4- sorted cells harbor intracellular CXCR4, which can be functionally expressed and provide NOD/SCID repopulation
Blood,
September 26, 2002;
100(8):
2778 - 2786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Jetmore, P. A. Plett, X. Tong, F. M. Wolber, R. Breese, R. Abonour, C. M. Orschell-Traycoff, and E. F. Srour
Homing efficiency, cell cycle kinetics, and survival of quiescent and cycling human CD34+ cells transplanted into conditioned NOD/SCID recipients
Blood,
March 1, 2002;
99(5):
1585 - 1593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Szilvassy, T. E. Meyerrose, P. L. Ragland, and B. Grimes
Differential homing and engraftment properties of hematopoietic progenitor cells from murine bone marrow, mobilized peripheral blood, and fetal liver
Blood,
October 1, 2001;
98(7):
2108 - 2115.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bautz, C. Denzlinger, L. Kanz, and R. Mohle
Chemotaxis and transendothelial migration of CD34+ hematopoietic progenitor cells induced by the inflammatory mediator leukotriene D4 are mediated by the 7-transmembrane receptor CysLT1
Blood,
June 1, 2001;
97(11):
3433 - 3440.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Kollet, A. Spiegel, A. Peled, I. Petit, T. Byk, R. Hershkoviz, E. Guetta, G. Barkai, A. Nagler, and T. Lapidot
Rapid and efficient homing of human CD34+CD38{-}/lowCXCR4+ stem and progenitor cells to the bone marrow and spleen of NOD/SCID and NOD/SCID/B2mnull mice
Blood,
May 15, 2001;
97(10):
3283 - 3291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Papayannopoulou, G. V. Priestley, B. Nakamoto, V. Zafiropoulos, L. M. Scott, and J. M. Harlan
Synergistic mobilization of hemopoietic progenitor cells using concurrent {beta}1 and {beta}2 integrin blockade or {beta}2-deficient mice
Blood,
March 1, 2001;
97(5):
1282 - 1288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Orschell-Traycoff, K. Hiatt, R. N. Dagher, S. Rice, M. C. Yoder, and E. F. Srour
Homing and engraftment potential of Sca-1+lin- cells fractionated on the basis of adhesion molecule expression and position in cell cycle
Blood,
August 15, 2000;
96(4):
1380 - 1387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Peled, O. Kollet, T. Ponomaryov, I. Petit, S. Franitza, V. Grabovsky, M. M. Slav, A. Nagler, O. Lider, R. Alon, et al.
The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34+ cells: role in transendothelial/stromal migration and engraftment of NOD/SCID mice
Blood,
June 1, 2000;
95(11):
3289 - 3296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Szilvassy, T. E. Meyerrose, and B. Grimes
Effects of cell cycle activation on the short-term engraftment properties of ex vivo expanded murine hematopoietic cells
Blood,
May 1, 2000;
95(9):
2829 - 2837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
