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Blood, Vol. 94 No. 9 (November 1), 1999:
pp. 3067-3076
Expression of CD10 by Human T Cells That Undergo Apoptosis Both In
Vitro and In Vivo
Giovanna Cutrona,
Nicolò Leanza,
Massimo Ulivi,
Giovanni Melioli,
Vito L. Burgio,
Giovanni Mazzarello,
Giovanni Gabutti,
Silvio Roncella, and
Manlio Ferrarini
From the Servizio di Immunologia Clinica and Servizio di Citometria
CBA, Istituto Nazionale per la Ricerca sul Cancro, IST Genoa; the
a Fondazione Andrea Cesalpino, Istituto di 1 Clinica Medica,
Università "La Sapienza," Rome; the Ia Clinica
Malattie Infettive, and the Istituto di Igiene, Università
di Genova, Genoa; the Servizio di Istologia e Anatomia Patologica,
Ospedale Sant'Andrea, La Spezia; and the Dipartimento di Oncologia,
Biologia e Genetica, Università di Genova, Genoa, Italy.
This study shows that human postthymic T cells express CD10 when
undergoing apoptosis, irrespective of the signal responsible for
initiating the apoptotic process. Cells from continuous T-cell lines
did not normally express CD10, but became CD10+ when
induced into apoptosis by human immunodeficiency virus (HIV) infection
and exposure to CD95 monoclonal antibody, etoposide, or staurosporin.
Inhibitors of caspases blocked apoptosis and CD10 expression. Both
CD4+ and CD8+ T cells purified from normal
peripheral blood expressed CD10 on apoptotic induction. CD10 was newly
synthesized by the apoptosing cells because its expression was
inhibited by exposure to cycloheximide and CD10 mRNA became detectable
by reverse transcription-polymerase chain reaction in T cells cultured
under conditions favoring apoptosis. To show CD10 on T cells apoptosing
in vivo, lymph node and peripheral blood T cells from
HIV+ subjects were used. These suspensions were composed
of a substantial, although variable, proportion of apoptosing T cells
that consistently expressed CD10. In contrast, CD10+ as
well as spontaneously apoptosing T cells were virtually absent in
peripheral blood from normal individuals. Collectively, these observations indicate that CD10 may represent a reliable marker for
identifying and isolating apoptosing T cells in vitro and ex vivo and
possibly suggest novel functions for surface CD10 in the apoptotic
process of lymphoid cells.
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The expression of CD10 by apoptotic lymphocytes is preceded by a pronounced externalization of phosphatidylserine
- John Bladon and Peter Taylor
Blood 2000 96: 4009.
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