|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
Blood, Vol. 95 No. 1 (January 1), 2000:
pp. 328-335
From INSERM U430 and Université Pierre et Marie Curie,
Hôpital Broussais, Paris, France.
Warm autoimmune hemolytic anemia (WAIHA) is characterized by an
accelerated clearance of red blood cells (RBCs) associated with the
presence of anti-RBC immunoglobulin (Ig)G autoantibodies. In the
present study, we analyzed the self-reactive IgG and IgM antibody
repertoires of patients with WAIHA using a technique of quantitative
immunoblotting on a panel of whole tissue extracts as sources of
self-antigens. Data were compared by means of multiparametric statistical analysis. We demonstrate that self-reactive antibody repertoires of IgG purified from plasma and of IgG purified from RBC
eluates do not differ between healthy donors and patients with WAIHA,
whereas autoreactive repertoires of IgM from patients exhibit broadly
altered patterns of reactivity as compared with those of healthy
controls. We further demonstrate that IgG purified from eluates of RBCs
of healthy donors induces agglutination of RBCs in an indirect Coombs
assay to a similar extent as IgG purified from eluates of RBCs of
patients with WAIHA. The capability of IgG to induce agglutination of
RBCs is suppressed in unfractionated eluates of healthy donors' cells,
whereas it is readily found in unfractionated eluates of patients'
RBCs. IgM is an essential factor in controlling the ability of IgG in
unfractionated RBC eluates to induce agglutination of RBCs. These
observations indicate that anti-RBC IgG autoantibodies of patients with
WAIHA share extensive similarity with natural antiRBC
autoantibodies of healthy donors and suggest that defective control
of IgG autoreactivity by autologous IgM is an underlying mechanism
for autoimmune hemolysis in WAIHA. (Blood. 2000;95:328-335)
This article has been cited by other articles:
| |||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 2000 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
