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Blood, Vol. 95 No. 10 (May 15), 2000:
pp. 3125-3132
Characterization of Ly-6M, a novel member of the Ly-6 family
of hematopoietic proteins
J. Megan M. Patterson,
Michael H. Johnson,
Drazen B. Zimonjic, and
Timothy A. Graubert
From the Division of Bone Marrow Transplantation and Stem Cell
Biology, Washington University School of Medicine, St Louis, MO; and
Laboratory of Experimental Carcinogenesis, Molecular Cytogenetics
Section, National Cancer Institute, National Institutes of Health,
Bethesda, MD.
The Ly-6 family includes a number of highly homologous,
low molecular weight glycophosphatidylinositol-linked proteins
expressed on hematopoietic and lymphoid cells. The best characterized
family member is Sca-1 (Ly-6A/E), an antigen commonly used for
purification of murine pluripotent hematopoietic cells. We sought to
characterize the genomic locus surrounding the Sca-1 gene. We
identified several overlapping P1 artificial chromosomes containing the
Sca-1 gene and mapped one of these to mouse chromosome
15D3.1-3.3, the region previously shown to contain members of the
murine Ly-6 gene family. We then mapped this clone and found
that the Sca-2 gene lies 35.4 kilobase (kb) downstream of
Sca-1 in the opposite transcriptional orientation. This is the
first direct demonstration of physical linkage of Ly-6 genes. A
novel gene, highly homologous to Sca-1 was identified and
localized 13.4 kb downstream of Sca-1. This gene, which we
designated Ly-6M, shares several structural features conserved
among members of the Ly-6 family. Ly-6M messenger RNA (mRNA) is
easily detectable in hematopoietic tissue (bone marrow, spleen, thymus,
peritoneal macrophages) as well as kidney and lung. No mRNA expression
was detected in heart, stomach, liver, small intestine, brain, or skin.
Ly-6M protein is detectable on 10% to 15% of peripheral blood
leukocytes, including monocytes and a subpopulation of
B220+ cells. Ly-6M is broadly distributed in the bone
marrow, with prominent expression on monocytes and myeloid precursors.
The identification and characterization of Ly-6M adds a new
member to a complex family of homologous, tightly linked genes that
have proven extremely useful reagents for defining populations within the hematopoietic system.
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