Blood, Vol. 95 No. 12 (June 15), 2000:
pp. 3758-3764
CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and
thrombopoietin production in bone marrow stromal cells
Anne Solanilla,
Julie Déchanet,
Abdel El Andaloussi,
Moryse Dupouy,
François Godard,
Jerome Chabrol,
Pierre Charbord,
Josy Reiffers,
Alan T. Nurden,
Babette Weksler,
Jean-François Moreau, and
Jean Ripoche
From the FR 60, Biologie des Greffes, Université de Bordeaux
2; ETS Franche Comté, Besançon; UMR 5533 CNRS, Pessac,
France; the Department of Medicine, the New York Hospital-Cornell
Medical Centre, New York, NY.
CD40 ligand (CD40L)/CD40 interactions play a central role in
T-cell-dependent B-cell activation as previously shown by in vitro
studies, the phenotype of CD40L knockout mice and the defective expression of CD40L in patients who have X-linked immunodeficiency with
hyper-IgM. The distribution of CD40 in cells other than of myeloid and
lymphoid lineages has suggested additional functions for this
receptor/ligand couple. Here we show that CD40L stimulates myelopoiesis
with a noticeable effect on megakaryocytopoiesis in cocultures of
hematopoietic progenitor cells and bone marrow stromal cells.
These results suggest a mechanism by which T-cell or
platelet-associated or soluble CD40L may regulate myelopoiesis.
