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Blood, Vol. 95 No. 12 (June 15), 2000:
pp. 3845-3852
Lineage-specific regulation of the murine RAG-2 promoter:
GATA-3 in T cells and Pax-5 in B cells
Hiroyuki Kishi,
Xing-Cheng Wei,
Zhe-Xiong Jin,
Yoshiyuki Fujishiro,
Takuya Nagata,
Tadashi Matsuda, and
Atsushi Muraguchi
From the Department of Immunology, Faculty of Medicine, Toyama
Medical and Pharmaceutical University, Toyama, Japan.
Recombination activating gene-1 (RAG-1) and
RAG-2 are expressed in lymphoid cells undergoing the antigen
receptor gene rearrangement. A study of the regulation of the mouse
RAG-2 promoter showed that the lymphocyte-specific promoter activity is
conferred 80 nucleotide (nt) upstream of RAG-2. Using an
electrophoretic mobility shift assay, it was shown that a
B-cell-specific transcription protein, Pax-5, and a T-cell-specific
transcription protein, GATA-3, bind to the  80 to 17 nt
region in B cells and T cells, respectively. Mutation of the RAG-2
promoter for Pax-5- and GATA-3-binding sites results in the reduction
of promoter activity in B cells and T cells. These results indicate
that distinct DNA binding proteins, Pax-5 and GATA-3, may regulate the
murine RAG-2 promoter in B and T lineage cells, respectively.
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